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Figure 3 Multi-crystal data collection from crystals of the membrane protein MAR. (a) A snapshot of a mesh micromount sample holder with the position marks for the crystals that were selected for data collection. (b, e) Two diffraction heat maps of the mesh scans separated by 60° of sample rotation. X and Y are the two axes of the scan; X is parallel to the goniometer rotation axis, whereas Y is the laboratory vertical axis. Units on the axes are scan steps, which are equal to the beam size (10 µm). (c, f) Two crystal maps for the mesh scans generated by Dozor-m2. Different colours are used to show distinct crystal areas, which are arbitrarily numbered, and the numbers are shown for each pixel; regions with overlapping diffraction patterns or contaminated by salt diffraction are shown in violet and numbered 999. (d) A sample diffraction image from the mesh scans. (g) Data-collection plots for three selected crystals. |