Figure 3 Structure of Deg8 (S292A) in comparison with DegP6 and Deg1 and oligomeric state analysis.
(a) Structures of Deg8 (S292A) (green and magenta), DegP6 (PDB entry 1ky9 ; red and orange; the second PDZ domain is not shown for clarity; Krojer et al., 2008 ) and Deg1 (PDB entry 3qo6 ; cyan and blue). Protease domains and PDZ domains are shown in cartoon and ribbon
modes, respectively. (b) The cavity formed in Deg8, DegP6 (PDB entry 1ky9 ; molecule B) and Deg1 (PDB entry 3qo6 ). (c) Structural comparison of protomers of Deg8 (S292A) (green) and Deg1 (PDB entry 3qo6 ; cyan); the difference in PDZ-domain orientations is indicated. (d) Analytical SEC of Deg8 at pH 6.0 (red line) and pH 8.0 (blue line). Different oligomeric
states are labelled above the peaks. |