Advances in Anaerobic Treatment: Research Paper
Effects of Ferric Hydroxide on Methanogenesis from Lipids and Long-Chain Fatty Acids in Anaerobic Digestion
Zhengkai Li,
Brian A. Wrenn,
Albert D. Venosa,
Zhengkai Li
Search for more papers by this authorBrian A. Wrenn
Search for more papers by this authorAlbert D. Venosa
Search for more papers by this authorZhengkai Li,
Brian A. Wrenn,
Albert D. Venosa,
Zhengkai Li
Search for more papers by this authorBrian A. Wrenn
Search for more papers by this authorAlbert D. Venosa
Search for more papers by this authorAbstract
The addition of ferric hydroxide to sludge from a municipal anaerobic digester stimulated the rate of methanogenesis from canola oil when the initial oil concentration was high (4600 mg/L; P < 0.002), but not when it was low (920 mg/L; P > 0.05). Similar trends were observed when oleic acid, a fatty acid that is a major component of canola oil triglycerides, was provided, but the effects were statistically significant only when the initial concentration of ferric hydroxide was also high (18 g/L; P = 0.015). Iron reduction occurred when ferric hydroxide was added to microcosms containing anaerobic digester sludge, but the extent of ferrous iron production was much less in acetate-amended microcosms than in those that were provided with canola oil or oleic acid. Methanogenesis and acetate consumption were completely inhibited when the initial acetate concentration was approximately 5000 mg/L, regardless of the initial ferric hydroxide concentration. The main effect of ferric hydroxide in this system appears to have been a result of stimulation of the rate of fatty acid oxidation.
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