ORIGINAL ARTICLE

Intra- and inter-laboratory comparison of mDixon and FastFung broths for Malassezia antifungal susceptibility testing

Abdourahim Abdillah

Aix-Marseille Université, IRD, AP-HM, SSA, VITROME, Marseille, France

IHU Méditerranée Infection, Marseille, France

Contribution: Conceptualization (equal), Data curation (equal), Formal analysis (equal), Investigation (equal), Methodology (equal), Visualization (equal), Writing - original draft (equal)

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Ann Packeu,

Ann Packeu

Service of Mycology and Aerobiology, Sciensano, Brussels, Belgium

BCCM/IHEM Fungal Collection, Service of Mycology and Aerobiology, Sciensano, Brussels, Belgium

Contribution: Conceptualization (equal), Data curation (equal), Investigation (equal), Methodology (equal), Visualization (equal), Writing - original draft (equal)

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Saber Khelaifia,

Saber Khelaifia

IHU Méditerranée Infection, Marseille, France

Contribution: Conceptualization (equal), Data curation (equal), Investigation (equal), Methodology (equal), Writing - original draft (equal)

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Stéphane Ranque,

Corresponding Author

Stéphane Ranque

[email protected]

orcid.org/0000-0003-3293-5276

Aix-Marseille Université, IRD, AP-HM, SSA, VITROME, Marseille, France

IHU Méditerranée Infection, Marseille, France

Correspondence

Pr Stéphane Ranque, VITROME, Aix Marseille Univ., IHU Méditerranée Infection. 19-21 Boulevard Jean Moulin, 13005 Marseille, France.

Email: [email protected]

Contribution: Conceptualization (equal), Formal analysis (equal), Investigation (equal), Methodology (equal), Resources (equal), Supervision (equal), Writing - review & editing (equal)

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Abdourahim Abdillah,

Abdourahim Abdillah

Aix-Marseille Université, IRD, AP-HM, SSA, VITROME, Marseille, France

IHU Méditerranée Infection, Marseille, France

Contribution: Conceptualization (equal), Data curation (equal), Formal analysis (equal), Investigation (equal), Methodology (equal), Visualization (equal), Writing - original draft (equal)

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Ann Packeu,

Ann Packeu

Service of Mycology and Aerobiology, Sciensano, Brussels, Belgium

BCCM/IHEM Fungal Collection, Service of Mycology and Aerobiology, Sciensano, Brussels, Belgium

Contribution: Conceptualization (equal), Data curation (equal), Investigation (equal), Methodology (equal), Visualization (equal), Writing - original draft (equal)

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Saber Khelaifia,

Saber Khelaifia

IHU Méditerranée Infection, Marseille, France

Contribution: Conceptualization (equal), Data curation (equal), Investigation (equal), Methodology (equal), Writing - original draft (equal)

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Stéphane Ranque,

Corresponding Author

Stéphane Ranque

[email protected]

orcid.org/0000-0003-3293-5276

Aix-Marseille Université, IRD, AP-HM, SSA, VITROME, Marseille, France

IHU Méditerranée Infection, Marseille, France

Correspondence

Pr Stéphane Ranque, VITROME, Aix Marseille Univ., IHU Méditerranée Infection. 19-21 Boulevard Jean Moulin, 13005 Marseille, France.

Email: [email protected]

Contribution: Conceptualization (equal), Formal analysis (equal), Investigation (equal), Methodology (equal), Resources (equal), Supervision (equal), Writing - review & editing (equal)

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First published: 23 March 2021

https://doi.org/10.1111/myc.13270

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Abstract

Background

Malassezia spp. antifungal susceptibility testing (AFST) capacities are limited by the lack of efficient and standardised AFST procedure, mainly because of the fastidious cultivation of these yeast.

Objectives

This study aimed to compare the FastFung broth (FFB) to modified Dixon broth (mDIXB) for the in vitro AFST of Malassezia spp. Fluconazole, ketoconazole, voriconazole and terbinafine MICs against a 19 Malassezia strains, including 6 M furfur, 4 M pachydermatis, 5 M sympodialis and 4 M slooffiae.

Methods

The essential agreement (EA) between the two assays, and the intra- and inter-laboratory agreement of each assay were assessed.

Results

The MIC data obtained in our study were comparable to those reported in the literature. FFB showed to enhance Malassezia growth and displayed 100% (±2-fold dilution) EAs demonstrating similar performances to mDIXB. In addition, the MIC data obtained by using the FFB were reproducible between laboratories with EAs ranging from 94.7% to 100%.

Conclusions

Therefore, FFB is a suitable alternative to mDXB for Malassezia spp. AFST.

CONFLICT OF INTEREST

The authors declare no conflict of interests.

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Volume64, Issue7

July 2021

Pages 716-720

Intra- and inter-laboratory comparison of mDixon and FastFung broths for Malassezia antifungal susceptibility testing

Abstract

Background

Objectives

Methods

Results

Conclusions

CONFLICT OF INTEREST

REFERENCES

References

Information

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Intra- and inter-laboratory comparison of mDixon and FastFung broths for Malassezia antifungal susceptibility testing

Abstract

Background

Objectives

Methods

Results

Conclusions

CONFLICT OF INTEREST

REFERENCES

References

Related

Information