The effect of chemotherapeutic agents on titanium-adherent biofilms
Victoria Ntrouka
Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam, Amsterdam, The Netherlands
Search for more papers by this authorMichel Hoogenkamp
Department of Cariology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam, Amsterdam, The Netherlands
Search for more papers by this authorEgija Zaura
Department of Cariology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam, Amsterdam, The Netherlands
Search for more papers by this authorFridus van der Weijden
Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam, Amsterdam, The Netherlands
Search for more papers by this authorVictoria Ntrouka
Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam, Amsterdam, The Netherlands
Search for more papers by this authorMichel Hoogenkamp
Department of Cariology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam, Amsterdam, The Netherlands
Search for more papers by this authorEgija Zaura
Department of Cariology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam, Amsterdam, The Netherlands
Search for more papers by this authorFridus van der Weijden
Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam, Amsterdam, The Netherlands
Search for more papers by this authorAbstract
Objective: To assess the effectiveness of different chemotherapeutic agents on biofilm-contaminated titanium surfaces.
Material and methods: This study used a recently described biofilm model. In experiment 1, Streptococcus mutans biofilms grown on titanium discs were treated with (1) EDTA, (2) citric acid (CA), (3) cetylpyridium chloride, (4) Ardox-X, (5) hydrogen peroxide (H2O2), (6) chlorhexidine (CHX) and (7) water. In experiment 2, polymicrobial biofilms were treated with (1) CA, (2) Ardox-X, (3) H2O2, (4) Ardox-X followed by CA, (5) H2O2 followed by CA, (6) CHX and (7) water. Aliquots of the suspended biofilms were plated and incubated anaerobically to enable counts of the total remaining viable bacteria, which were expressed as CFUs. Following incubation, the amount of protein remaining in the treated S. mutans biofilms was quantified to assess the removal potency of each treatment agent.
Results: H2O2, Ardox-X and CA killed significantly more S. mutans compared with the other treatments. H2O2 and CA removed significantly more protein than water. CA and the combination treatments were significantly more effective against the polymicrobial biofilms than CHX, H2O2 and Ardox-X. The difference in the killing efficacy between CA alone and the combination treatments was not statistically significant.
Conclusion: Among the chemicals tested, CA demonstrated the greatest decontamination capacity with respect to both the killing and the removal of biofilm cells. This combination of effects is clinically desirable because it promotes biocompatibility and healing around a previously contaminated implant surface. These results should, however, be validated in in vivo studies.
To cite this article: Ntrouka V, Hoogenkamp M, Zaura E, van der Weijden F. The effect of chemotherapeutic agents on titanium-adherent biofilms.Clin. Oral Impl. Res. 22, 2011; 1227–1234.doi: 10.1111/j.1600-0501.2010.02085.x
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