Water channels in platelet volume regulation
Jin-Sook Lee
Department of Physiology, Wayne State University School of Medicine, Detroit, MI, USA
Search for more papers by this authorShivani Agrawal
Department of Physiology, Wayne State University School of Medicine, Detroit, MI, USA
Search for more papers by this authorMichele von Turkovich
Department of Pathology, University of Vermont College of Medicine, Burlington, VT, USA
Search for more papers by this authorDouglas J. Taatjes
Department of Pathology, University of Vermont College of Medicine, Burlington, VT, USA
Search for more papers by this authorCorresponding Author
Daniel A. Walz
Department of Physiology, Wayne State University School of Medicine, Detroit, MI, USA
Bhanu P. JENA, Department of Physiology, Wayne State University School of Medicine, 5245 Scott Hall, 540 E. Canfield, Detroit, MI 48201, USA. Tel.: +1-313-577-1532 Fax: +1-313-993-4177 E-mail: [email protected]Daniel A. WALZ, Department of Physiology, Wayne State University School of Medicine, 1261 Scott Hall, 540 E. Canfield, Detroit, MI 48201, USA. Tel.: +1-313-577-7946 Fax: +1-313-993-9399 E-mail: [email protected]Search for more papers by this authorCorresponding Author
Bhanu P. Jena
Department of Physiology, Wayne State University School of Medicine, Detroit, MI, USA
Bhanu P. JENA, Department of Physiology, Wayne State University School of Medicine, 5245 Scott Hall, 540 E. Canfield, Detroit, MI 48201, USA. Tel.: +1-313-577-1532 Fax: +1-313-993-4177 E-mail: [email protected]Daniel A. WALZ, Department of Physiology, Wayne State University School of Medicine, 1261 Scott Hall, 540 E. Canfield, Detroit, MI 48201, USA. Tel.: +1-313-577-7946 Fax: +1-313-993-9399 E-mail: [email protected]Search for more papers by this authorJin-Sook Lee
Department of Physiology, Wayne State University School of Medicine, Detroit, MI, USA
Search for more papers by this authorShivani Agrawal
Department of Physiology, Wayne State University School of Medicine, Detroit, MI, USA
Search for more papers by this authorMichele von Turkovich
Department of Pathology, University of Vermont College of Medicine, Burlington, VT, USA
Search for more papers by this authorDouglas J. Taatjes
Department of Pathology, University of Vermont College of Medicine, Burlington, VT, USA
Search for more papers by this authorCorresponding Author
Daniel A. Walz
Department of Physiology, Wayne State University School of Medicine, Detroit, MI, USA
Bhanu P. JENA, Department of Physiology, Wayne State University School of Medicine, 5245 Scott Hall, 540 E. Canfield, Detroit, MI 48201, USA. Tel.: +1-313-577-1532 Fax: +1-313-993-4177 E-mail: [email protected]Daniel A. WALZ, Department of Physiology, Wayne State University School of Medicine, 1261 Scott Hall, 540 E. Canfield, Detroit, MI 48201, USA. Tel.: +1-313-577-7946 Fax: +1-313-993-9399 E-mail: [email protected]Search for more papers by this authorCorresponding Author
Bhanu P. Jena
Department of Physiology, Wayne State University School of Medicine, Detroit, MI, USA
Bhanu P. JENA, Department of Physiology, Wayne State University School of Medicine, 5245 Scott Hall, 540 E. Canfield, Detroit, MI 48201, USA. Tel.: +1-313-577-1532 Fax: +1-313-993-4177 E-mail: [email protected]Daniel A. WALZ, Department of Physiology, Wayne State University School of Medicine, 1261 Scott Hall, 540 E. Canfield, Detroit, MI 48201, USA. Tel.: +1-313-577-7946 Fax: +1-313-993-9399 E-mail: [email protected]Search for more papers by this authorAbstract
The regulation of platelet volume significantly affects its function. Because water is the major molecule in cells and its active transport via water channels called aquaporins (AQPs) have been implicated in cellular and organelle volume regulation, the presence of water channels in platelets and their potential role in platelet volume regulation was investigated. G-protein–mediated AQP regulation in secretory vesicle swelling has previously been reported in neurons and in pancreatic acinar cells. Mercuric chloride has been demonstrated to inhibit most AQPs except AQP6, which is stimulated by the compound. Exposure of platelets to HgCl2-induced swelling in a dose-dependent manner, suggesting the presence of AQP6 in platelets. Immunoblot analysis of platelet protein confirmed the presence of AQP6, and also of Gαo, Gαi–1 and Gαi–3 proteins. Results from this study demonstrate for the first time that in platelets AQP6 is involved in cell volume regulation via a G-protein–mediated pathway.
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