Purification, crystallization and preliminary X-ray diffraction studies on pyruvate phosphate dikinase from maize
Pyruvate phosphate dikinase
Hiroaki Kato, Kinetic Crystallography Research Team, RIKEN Harima Institute at SPring-8, 1-1-1 Kouto, Mikazuki-cho, Sayo-gun, Hyogo 679-5148, Japan. E-mail: [email protected]
Abstract
Pyruvate phosphate dikinase (PPDK) from maize catalyzes the reversible conversion of ATP, orthophosphate and pyruvate to AMP, pyrophosphate and PEP. In higher plants, this enzyme is believed to be involved in the C4 dicarboxylic acid pathway. PPDK was crystallized by the vapour-diffusion method using polyethylene glycol as a precipitant. The crystals belong to the orthorhombic space group C2, with unit-cell parameters a = 108.2, b = 100.2, c = 108.4 Å, β = 96.5°, and diffract to 2.3 Å using SPring-8 synchrotron radiation.
