Accumulation of CCR4-expressing CD4+ T cells and high concentration of its ligands (TARC and MDC) in bronchoalveolar lavage fluid of patients with eosinophilic pneumonia
S. Katoh
Third Department of Internal Medicine, Miyazaki Medical College, Miyazaki;
Search for more papers by this authorN. Matsumoto
Third Department of Internal Medicine, Miyazaki Medical College, Miyazaki;
Search for more papers by this authorK. Matsumoto
Third Department of Internal Medicine, Miyazaki Medical College, Miyazaki;
Search for more papers by this authorK. Abe
Third Department of Internal Medicine, Miyazaki Medical College, Miyazaki;
Search for more papers by this authorN. Onai
Department of Molecular Preventive Medicine, The University of Tokyo, Graduate School of Medicine, Tokyo, Japan
Search for more papers by this authorK. Matsushima
Department of Molecular Preventive Medicine, The University of Tokyo, Graduate School of Medicine, Tokyo, Japan
Search for more papers by this authorS. Matsukura
Third Department of Internal Medicine, Miyazaki Medical College, Miyazaki;
Search for more papers by this authorS. Katoh
Third Department of Internal Medicine, Miyazaki Medical College, Miyazaki;
Search for more papers by this authorN. Matsumoto
Third Department of Internal Medicine, Miyazaki Medical College, Miyazaki;
Search for more papers by this authorK. Matsumoto
Third Department of Internal Medicine, Miyazaki Medical College, Miyazaki;
Search for more papers by this authorK. Abe
Third Department of Internal Medicine, Miyazaki Medical College, Miyazaki;
Search for more papers by this authorN. Onai
Department of Molecular Preventive Medicine, The University of Tokyo, Graduate School of Medicine, Tokyo, Japan
Search for more papers by this authorK. Matsushima
Department of Molecular Preventive Medicine, The University of Tokyo, Graduate School of Medicine, Tokyo, Japan
Search for more papers by this authorS. Matsukura
Third Department of Internal Medicine, Miyazaki Medical College, Miyazaki;
Search for more papers by this authorAbstract
Background: Th2 cells are thought to be involved in eosinophilic inflammation of the lung. CC chemokine receptor 4 (CCR4) has been identified as a specific receptor for both thymus and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC), and is preferentially expressed on Th2 cells.
Objective: Our aim was to evaluate the role of Th2 cells in the lung of patients with eosinophilic pneumonia (EP).
Methods: The concentrations of TARC, MDC, and interleukin (IL)-5 were measured in bronchoalveolar lavage fluid (BALF) by ELISA. Proportion of CCR4-expressing CD4+ T cells (CCR4+ CD4+ T cells) was determined by flow cytometry.
Results: TARC and MDC concentrations in BALF were higher in patients with EP than in normal subjects. The proportion of CCR4-expressing cells among CD4+ T cells was higher in BALF than in peripheral blood of patients with EP. There was a significant correlation between the number of CCR4+ CD4+ T cells and the levels of TARC, MDC, and IL-5 in BALF of patients with EP.
Conclusions: Our data suggest that Th2 cells, which express CCR4 and its ligands (TARC and MDC), contribute to the pathogenesis of EP in the lung.
References
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