Electroactive Silica Nanoparticles for Biological Labeling†
This work was supported by a laboratory-directed research and development program at Pacific Northwest National Laboratory (PNNL). The work was performed at the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the U.S. Department of Energy (DOE) and located at PNNL. PNNL is operated by Battelle for the DOE under Contract DE-AC05-76 L01830.
Graphical Abstract
Making biosense: Electroactive poly(guanine)-functionalized silica nanoparticles have been synthesized and used as biological labels (see scheme). An electrochemical immunobiosensor based on such labels was developed, which utilizes a mediator-generated catalytic reaction. This immunobiosensor is very sensitive for IgG detection (to a limit of 0.2 ng mL−1 or 1.3 pM), which was attributed to signal amplification from the poly(guanine)-functionalized silica nanoparticles and from the catalytic oxidation of guanine.
