Experimental and computational examination of anastellin (FnIII1c)–polymer interactions
David Mallinson
Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), University of Strathclyde, 161 Cathedral Street, Glasgow, United Kingdom
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David L. Cheung
School of Chemistry, National University of Ireland, Galway, University Road, Galway, Ireland
Correspondence to: D. L. Cheung, Z. J. Zhang, and D. A. Lamprou; e-mail: [email protected], [email protected], and [email protected]Search for more papers by this authorDorin Simionesie
School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, United Kingdom
Search for more papers by this authorAlexander B. Mullen
Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), University of Strathclyde, 161 Cathedral Street, Glasgow, United Kingdom
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Zhenyu J. Zhang
School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, United Kingdom
Correspondence to: D. L. Cheung, Z. J. Zhang, and D. A. Lamprou; e-mail: [email protected], [email protected], and [email protected]Search for more papers by this authorCorresponding Author
Dimitrios A. Lamprou
Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), University of Strathclyde, 161 Cathedral Street, Glasgow, United Kingdom
Centre for Innovative Manufacturing in Continuous Manufacturing and Crystallisation (CMAC), University of Strathclyde, Glasgow, United Kingdom
Correspondence to: D. L. Cheung, Z. J. Zhang, and D. A. Lamprou; e-mail: [email protected], [email protected], and [email protected]Search for more papers by this authorDavid Mallinson
Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), University of Strathclyde, 161 Cathedral Street, Glasgow, United Kingdom
Search for more papers by this authorCorresponding Author
David L. Cheung
School of Chemistry, National University of Ireland, Galway, University Road, Galway, Ireland
Correspondence to: D. L. Cheung, Z. J. Zhang, and D. A. Lamprou; e-mail: [email protected], [email protected], and [email protected]Search for more papers by this authorDorin Simionesie
School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, United Kingdom
Search for more papers by this authorAlexander B. Mullen
Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), University of Strathclyde, 161 Cathedral Street, Glasgow, United Kingdom
Search for more papers by this authorCorresponding Author
Zhenyu J. Zhang
School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, United Kingdom
Correspondence to: D. L. Cheung, Z. J. Zhang, and D. A. Lamprou; e-mail: [email protected], [email protected], and [email protected]Search for more papers by this authorCorresponding Author
Dimitrios A. Lamprou
Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), University of Strathclyde, 161 Cathedral Street, Glasgow, United Kingdom
Centre for Innovative Manufacturing in Continuous Manufacturing and Crystallisation (CMAC), University of Strathclyde, Glasgow, United Kingdom
Correspondence to: D. L. Cheung, Z. J. Zhang, and D. A. Lamprou; e-mail: [email protected], [email protected], and [email protected]Search for more papers by this authorAbstract
Using a combination of experimental and computational approaches, the interaction between anastellin, a recombinant fragment of fibronectin, and representative biomaterial surfaces has been examined. Anastellin and superfibronectin have been seen to exhibit antiangiogenic properties and other properties that may make it suitable for consideration for incorporation into biomaterials. The molecular interaction was directly quantified by atomic force microscope (AFM)-based force spectroscopy, complemented by adsorption measurements using quartz crystal microbalance (QCM). Using AFM, it was found that the anastellin molecule facilitates a stronger adhesion on polyurethane films (72.0 pN nm−1) than on poly (methyl methacrylate) films (68.6 pN nm−1). However, this is not consistent with the QCM adsorption measurements, which show no significant difference. Molecular dynamics simulations of the behavior of anastellin on polyurethane in aqueous solution were performed to rationalize the experimental data, and show that anastellin is capable of rapid adsorption to PU while its secondary structure is stable upon adsorption in water. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 737–745, 2017.
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