Chapter 29
Confocal and Conventional Fluorescence-Based High Throughput Screening in Protein Engineering
Ulrich Haupts,
Oliver Hesse,
Michael Strerath,
Peter J. Walla,
Wayne M. Coco,
Ulrich Haupts
DIREVO Biotech AG, Nattermannalle 1, 50829 Cologne, Germany
Search for more papers by this authorOliver Hesse
DIREVO Biotech AG, Nattermannalle 1, 50829 Cologne, Germany
Search for more papers by this authorMichael Strerath
DIREVO Biotech AG, Nattermannalle 1, 50829 Cologne, Germany
Search for more papers by this authorPeter J. Walla
Technische Universität Braunschweig, Institute for Physical and Theoretical Chemistry, Dept. for Biophysical Chemistry, Hans-Sommerstr. 10, 38106 Brunswick, Germany
Max-Planck-Institute for Biophysical Chemistry, Am Faßberg 11, 37077 Göttingen, Germany
Search for more papers by this authorWayne M. Coco
DIREVO Biotech AG, Nattermannalle 1, 50829 Cologne, Germany
Search for more papers by this authorUlrich Haupts,
Oliver Hesse,
Michael Strerath,
Peter J. Walla,
Wayne M. Coco,
Ulrich Haupts
DIREVO Biotech AG, Nattermannalle 1, 50829 Cologne, Germany
Search for more papers by this authorOliver Hesse
DIREVO Biotech AG, Nattermannalle 1, 50829 Cologne, Germany
Search for more papers by this authorMichael Strerath
DIREVO Biotech AG, Nattermannalle 1, 50829 Cologne, Germany
Search for more papers by this authorPeter J. Walla
Technische Universität Braunschweig, Institute for Physical and Theoretical Chemistry, Dept. for Biophysical Chemistry, Hans-Sommerstr. 10, 38106 Brunswick, Germany
Max-Planck-Institute for Biophysical Chemistry, Am Faßberg 11, 37077 Göttingen, Germany
Search for more papers by this authorWayne M. Coco
DIREVO Biotech AG, Nattermannalle 1, 50829 Cologne, Germany
Search for more papers by this authorBook Editor(s):Prof. Dr. Stefan Lutz,
Prof. Dr. Uwe T. Bornscheuer,
Prof. Dr. Stefan Lutz
Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA 30322, USA
Search for more papers by this authorProf. Dr. Uwe T. Bornscheuer
Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany
Search for more papers by this authorFirst published: 12 November 2008
Summary
This chapter contains sections titled:
-
General Aspects
-
Fluorescence
-
Hardware and Instrumentation
-
Practical Considerations and Screening Protocol
-
Challenges and Future Directions
-
Abbreviations
-
Acknowledgments
-
References
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