Erratum to “Epithelial-Mesenchymal Transition Promotes the Differentiation Potential of Xenopus tropicalis Immature Sertoli Cells”
First published: 06 August 2019
In the article titled “Epithelial-Mesenchymal Transition Promotes the Differentiation Potential of Xenopus tropicalis Immature Sertoli Cells” [1], due to a production error, the horizontal description of Figure 3(c) was missing. Also, Figure 7(b) was incorrectly replaced by Figure 8(b). The correct figures are as follows:
Figure 3 (a)
GSK-3 inhibitor (CHIR99021) stimulates EMT in XtiSC cell culture. XtiSCs were cultured in a growth medium supplemented with 25 ng/ml FGF2 or 3 μM CHIR99021 or 0.1% DMSO as a control. (a) After three-day treatment, the morphological change of XtiSCs from cobblestone shape to a long-rod shape in a medium with CHIR99021 was observed. Scale bar: 100 μm. (b, c) Immunofluorescent staining and RT-PCR analysis showed the downregulation of epithelial markers (cytokeratin, β-catenin at the plasma cell membrane) (b) and the increase of mesenchymal markers (fibronectin, integrin α5β1, Snai1, and twist1) (c, d). Arrows show the expression of β-catenin at the plasma cell membrane. Nuclei stained with DAPI. Scale bar: 20 μm. Results are representative of three biological replicates; ∗p < 0.005, ∗∗p < 0.001.
Figure 3 (b)
GSK-3 inhibitor (CHIR99021) stimulates EMT in XtiSC cell culture. XtiSCs were cultured in a growth medium supplemented with 25 ng/ml FGF2 or 3 μM CHIR99021 or 0.1% DMSO as a control. (a) After three-day treatment, the morphological change of XtiSCs from cobblestone shape to a long-rod shape in a medium with CHIR99021 was observed. Scale bar: 100 μm. (b, c) Immunofluorescent staining and RT-PCR analysis showed the downregulation of epithelial markers (cytokeratin, β-catenin at the plasma cell membrane) (b) and the increase of mesenchymal markers (fibronectin, integrin α5β1, Snai1, and twist1) (c, d). Arrows show the expression of β-catenin at the plasma cell membrane. Nuclei stained with DAPI. Scale bar: 20 μm. Results are representative of three biological replicates; ∗p < 0.005, ∗∗p < 0.001.
Figure 3 (c)
GSK-3 inhibitor (CHIR99021) stimulates EMT in XtiSC cell culture. XtiSCs were cultured in a growth medium supplemented with 25 ng/ml FGF2 or 3 μM CHIR99021 or 0.1% DMSO as a control. (a) After three-day treatment, the morphological change of XtiSCs from cobblestone shape to a long-rod shape in a medium with CHIR99021 was observed. Scale bar: 100 μm. (b, c) Immunofluorescent staining and RT-PCR analysis showed the downregulation of epithelial markers (cytokeratin, β-catenin at the plasma cell membrane) (b) and the increase of mesenchymal markers (fibronectin, integrin α5β1, Snai1, and twist1) (c, d). Arrows show the expression of β-catenin at the plasma cell membrane. Nuclei stained with DAPI. Scale bar: 20 μm. Results are representative of three biological replicates; ∗p < 0.005, ∗∗p < 0.001.
Figure 3 (d)
GSK-3 inhibitor (CHIR99021) stimulates EMT in XtiSC cell culture. XtiSCs were cultured in a growth medium supplemented with 25 ng/ml FGF2 or 3 μM CHIR99021 or 0.1% DMSO as a control. (a) After three-day treatment, the morphological change of XtiSCs from cobblestone shape to a long-rod shape in a medium with CHIR99021 was observed. Scale bar: 100 μm. (b, c) Immunofluorescent staining and RT-PCR analysis showed the downregulation of epithelial markers (cytokeratin, β-catenin at the plasma cell membrane) (b) and the increase of mesenchymal markers (fibronectin, integrin α5β1, Snai1, and twist1) (c, d). Arrows show the expression of β-catenin at the plasma cell membrane. Nuclei stained with DAPI. Scale bar: 20 μm. Results are representative of three biological replicates; ∗p < 0.005, ∗∗p < 0.001.
Figure 7 (a)
In vivo differentiation of EMT-induced XtiSCs into cardiomyocytes. (a) Experimental scheme. RFP-positive XtiSCs were cultured in a growth medium supplemented with 3 μM CHIR99021 or 0.1% DMSO as a control for 3-4 days before transplantation into 2-day-old tadpoles. (b) At the 4th, 14th, or 30th day postinjection (dpi), tadpoles were fixed and sectioned for double staining with antibodies against red fluorescent protein and cardiac troponin T labeling cardiomyocytes in the heart. Scale bar: 20 μm. Nuclei stained with DAPI. Results are representative of four biological replicates.
Figure 7 (b)
In vivo differentiation of EMT-induced XtiSCs into cardiomyocytes. (a) Experimental scheme. RFP-positive XtiSCs were cultured in a growth medium supplemented with 3 μM CHIR99021 or 0.1% DMSO as a control for 3-4 days before transplantation into 2-day-old tadpoles. (b) At the 4th, 14th, or 30th day postinjection (dpi), tadpoles were fixed and sectioned for double staining with antibodies against red fluorescent protein and cardiac troponin T labeling cardiomyocytes in the heart. Scale bar: 20 μm. Nuclei stained with DAPI. Results are representative of four biological replicates.
