The contribution of oxidative stress to platelet senescence during storage
Li Wang
Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts
Department of Dermatology, Harvard Medical School, Boston, Massachusetts
Search for more papers by this authorRufeng Xie
Blood Engineering Laboratory, Shanghai Blood Center, Shanghai, China
Search for more papers by this authorZhijia Fan
Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China
Search for more papers by this authorJie Yang
Blood Engineering Laboratory, Shanghai Blood Center, Shanghai, China
Search for more papers by this authorWei Liang
Department of Laboratory Medicine, The Second People's Hospital of Lianyungang City, Jiangsu Province, China
Search for more papers by this authorQiang Wu
Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China
Department of Clinical Laboratory Medicine, Shanghai Fifth People's Hospital, Fudan University, Shanghai, China
Search for more papers by this authorMei X. Wu
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts
Department of Dermatology, Harvard Medical School, Boston, Massachusetts
Search for more papers by this authorCorresponding Author
Zhicheng Wang
Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts
Department of Dermatology, Harvard Medical School, Boston, Massachusetts
Address reprint requests to: Zhicheng Wang and Yuan Lu, Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China; e-mail: [email protected] (ZCW); [email protected] (YL)Search for more papers by this authorCorresponding Author
Yuan Lu
Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China
Address reprint requests to: Zhicheng Wang and Yuan Lu, Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China; e-mail: [email protected] (ZCW); [email protected] (YL)Search for more papers by this authorLi Wang
Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts
Department of Dermatology, Harvard Medical School, Boston, Massachusetts
Search for more papers by this authorRufeng Xie
Blood Engineering Laboratory, Shanghai Blood Center, Shanghai, China
Search for more papers by this authorZhijia Fan
Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China
Search for more papers by this authorJie Yang
Blood Engineering Laboratory, Shanghai Blood Center, Shanghai, China
Search for more papers by this authorWei Liang
Department of Laboratory Medicine, The Second People's Hospital of Lianyungang City, Jiangsu Province, China
Search for more papers by this authorQiang Wu
Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China
Department of Clinical Laboratory Medicine, Shanghai Fifth People's Hospital, Fudan University, Shanghai, China
Search for more papers by this authorMei X. Wu
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts
Department of Dermatology, Harvard Medical School, Boston, Massachusetts
Search for more papers by this authorCorresponding Author
Zhicheng Wang
Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts
Department of Dermatology, Harvard Medical School, Boston, Massachusetts
Address reprint requests to: Zhicheng Wang and Yuan Lu, Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China; e-mail: [email protected] (ZCW); [email protected] (YL)Search for more papers by this authorCorresponding Author
Yuan Lu
Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China
Address reprint requests to: Zhicheng Wang and Yuan Lu, Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China; e-mail: [email protected] (ZCW); [email protected] (YL)Search for more papers by this authorAbstract
BACKGROUND
Platelets for transfusion become senescent and dysfunctional during storage, resulting in a markedly short shelf life (5 days). We hypothesized that oxidative stress might account for this decline.
STUDY DESIGN AND METHODS
Human platelets were treated with or without antioxidants before storage, and samples were collected and analyzed at different time points. Platelet senescence was determined by senescence-associated β-galactosidase assay, and senescence-related platelet qualities were also analyzed.
RESULTS
Sign of senescence became evident after Day 3 and continued to increase over time. We also found that chemical induction of platelet activation did not affect senescence level, whereas apoptosis inducers showed a stimulative effect on platelet senescence. Moreover, this effect was not prevented by a pan-caspase inhibitor. Meanwhile, cellular and mitochondrial reactive oxygen species were found elevated during storage, and treatments with antioxidants successfully prevented this increase and also mitigated senescence levels of stored platelets. Finally, resveratrol, a natural antioxidant, was utilized as a novel storage additive to safely extend platelet shelf time. We showed that the addition of resveratrol efficiently postponed platelet senescence and ameliorated platelet storage lesion.
CONCLUSIONS
Platelets during storage became senescent and dysfunctional over time, and we found that oxidative stress might account for this decline. The addition of antioxidants effectively postponed senescence and ameliorated platelet storage lesion, which might provide a valuable reference to future platelet storage methodologies.
CONFLICT OF INTERESTS
The authors have disclosed no conflicts of interest.
Supporting Information
| Filename | Description |
|---|---|
| trf15291-sup-0001-FigureS1.docxWord 2007 document , 484.3 KB | Figure S1. Resveratrol did not affect platelet metabolic levels during storage. Platelets were treated with vehicle or 50 μM of resveratrol before storage and stored for up to 7 days at the standard blood banking conditions. At the desired time points, (A) MPV, (B) platelet count, (C) pH, and (D) glucose level were measured. Mean ± standard error of the mean. n = 3 (B and D), n = 5 (C). Significance was determined by two-way analysis of variance with Dunnett's posttest. *p < 0.05, **p < 0.01, ***p < 0.001. |
| trf15291-sup-0002-TableS1.xlsxExcel 2007 spreadsheet , 189.6 KB | Table S1. The TMT LC-MS/MS proteomic analysis during platelets storage |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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