Serum Helicobacter pylori antibody reactivity in seven Asian countries using an automated latex aggregation turbidity assay
Junko Akada
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Search for more papers by this authorEvariste Tshibangu-Kabamba
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Present Address: Evariste Tshibangu-Kabamba, Department of Parasitology, Graduate School of Medicine, Osaka City University, Osaka, Japan; Yuichi Matsuo, Department of Biomedical Laboratory Sciences, Kumamoto University, Kumamoto, Japan; Shamshul Ansari, Department of Microbiology, Chitwan Medical College, Bharatpur, Nepal; Phawinee Subsomwong, Department of Microbiology and Immunology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan.Search for more papers by this authorVo Phuoc Tuan
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Department of Endoscopy, Cho Ray Hospital, Ho Chi Minh City, Vietnam
Search for more papers by this authorShusaku Kurogi
Clinical Laboratory Center, Oita University Hospital, Yufu, Japan
Department of Molecular Pathology, Faculty of Medicine, Oita University, Yufu, Japan
Search for more papers by this authorYuichi Matsuo
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Present Address: Evariste Tshibangu-Kabamba, Department of Parasitology, Graduate School of Medicine, Osaka City University, Osaka, Japan; Yuichi Matsuo, Department of Biomedical Laboratory Sciences, Kumamoto University, Kumamoto, Japan; Shamshul Ansari, Department of Microbiology, Chitwan Medical College, Bharatpur, Nepal; Phawinee Subsomwong, Department of Microbiology and Immunology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan.Search for more papers by this authorShamshul Ansari
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Present Address: Evariste Tshibangu-Kabamba, Department of Parasitology, Graduate School of Medicine, Osaka City University, Osaka, Japan; Yuichi Matsuo, Department of Biomedical Laboratory Sciences, Kumamoto University, Kumamoto, Japan; Shamshul Ansari, Department of Microbiology, Chitwan Medical College, Bharatpur, Nepal; Phawinee Subsomwong, Department of Microbiology and Immunology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan.Search for more papers by this authorDalla Doohan
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia
Search for more papers by this authorBui Hoang Phuc
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Search for more papers by this authorPhawinee Subsomwong
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Present Address: Evariste Tshibangu-Kabamba, Department of Parasitology, Graduate School of Medicine, Osaka City University, Osaka, Japan; Yuichi Matsuo, Department of Biomedical Laboratory Sciences, Kumamoto University, Kumamoto, Japan; Shamshul Ansari, Department of Microbiology, Chitwan Medical College, Bharatpur, Nepal; Phawinee Subsomwong, Department of Microbiology and Immunology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan.Search for more papers by this authorLanggeng Agung Waskito
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia
Search for more papers by this authorTran Thanh Binh
Department of Endoscopy, Cho Ray Hospital, Ho Chi Minh City, Vietnam
Search for more papers by this authorLam Tung Nguyen
Department of Hepatogastroenterology, 108 Military Central Hospital, Hanoi, Vietnam
Search for more papers by this authorVu Van Khien
Department of Hepatogastroenterology, 108 Military Central Hospital, Hanoi, Vietnam
Search for more papers by this authorHo Dang Quy Dung
Department of Endoscopy, Cho Ray Hospital, Ho Chi Minh City, Vietnam
Search for more papers by this authorMuhammad Miftahussurur
Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia
Gastroentero-Hepatology Division, Department of Internal Medicine, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Airlangga University, Surabaya, Indonesia
Search for more papers by this authorAri Fahrial Syam
Division of Gastroenterology, Department of Intestinal Medicine, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
Search for more papers by this authorLotay Tshering
Department of Surgery, Jigme Dorji Wangchuck National Referral Hospital, Thimphu, Bhutan
Search for more papers by this authorRatha-korn Vilaichone
Gastroenterology Unit, Digestive Diseases Research Center, Thammasat University Hospital, Khlong Luang, Thailand
Department of Medicine, Chulabhorn International College of Medicine, Thammasat University Hospital, Khlong Luang, Thailand
Search for more papers by this authorVarocha Mahachai
GI and Liver Center, Bangkok Medical Center, Bangkok, Thailand
Search for more papers by this authorThawee Ratanachu-ek
Department of Surgery, Rajavithi Hospital, Bangkok, Thailand
Search for more papers by this authorPradeep Krishna Shrestha
Department of Gastroenterology, Maharajgunj Medical Campus, Tribhuvan University Teaching Hospital, Kathmandu, Nepal
Search for more papers by this authorThan Than Yee
Department of GI and HBP Surgery, No (2) Defense Service General Hospital, Nay Pyi Taw, Myanmar
Search for more papers by this authorKyaw Htet
Department of GI and HBP Surgery, No (1) Defense Service General Hospital, Mingaladon, Myanmar
Search for more papers by this authorHafeza Aftab
Department of Gastroenterology, Dhaka Medical Collage and Hospital, Dhaka, Bangladesh
Search for more papers by this authorTakeshi Matsuhisa
Department of Gastroenterology, Tama-Nagayama University Hospital, Nippon Medical School, Tama, Japan
Search for more papers by this authorTomohisa Uchida
Department of Molecular Pathology, Faculty of Medicine, Oita University, Yufu, Japan
Search for more papers by this authorTadayoshi Okimoto
Department of Gastroenterology, Faculty of Medicine, Oita University, Yufu, Japan
Search for more papers by this authorKazuhiro Mizukami
Department of Gastroenterology, Faculty of Medicine, Oita University, Yufu, Japan
Search for more papers by this authorMasaaki Kodama
Department of Gastroenterology, Faculty of Medicine, Oita University, Yufu, Japan
Faculty of Welfare and Health Science, Oita University, Yufu, Japan
Search for more papers by this authorKazunari Murakami
Department of Gastroenterology, Faculty of Medicine, Oita University, Yufu, Japan
Search for more papers by this authorNaohiko Takahashi
Clinical Laboratory Center, Oita University Hospital, Yufu, Japan
Search for more papers by this authorCorresponding Author
Yoshio Yamaoka
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Global Oita Medical Advanced Research Center for Health, Oita University, Yufu, Japan
Department of Medicine, Gastroenterology and Hepatology Section, Baylor College of Medicine, Houston, Texas, USA
Correspondence
Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan.
Email: [email protected]
Search for more papers by this authorJunko Akada
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Search for more papers by this authorEvariste Tshibangu-Kabamba
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Present Address: Evariste Tshibangu-Kabamba, Department of Parasitology, Graduate School of Medicine, Osaka City University, Osaka, Japan; Yuichi Matsuo, Department of Biomedical Laboratory Sciences, Kumamoto University, Kumamoto, Japan; Shamshul Ansari, Department of Microbiology, Chitwan Medical College, Bharatpur, Nepal; Phawinee Subsomwong, Department of Microbiology and Immunology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan.Search for more papers by this authorVo Phuoc Tuan
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Department of Endoscopy, Cho Ray Hospital, Ho Chi Minh City, Vietnam
Search for more papers by this authorShusaku Kurogi
Clinical Laboratory Center, Oita University Hospital, Yufu, Japan
Department of Molecular Pathology, Faculty of Medicine, Oita University, Yufu, Japan
Search for more papers by this authorYuichi Matsuo
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Present Address: Evariste Tshibangu-Kabamba, Department of Parasitology, Graduate School of Medicine, Osaka City University, Osaka, Japan; Yuichi Matsuo, Department of Biomedical Laboratory Sciences, Kumamoto University, Kumamoto, Japan; Shamshul Ansari, Department of Microbiology, Chitwan Medical College, Bharatpur, Nepal; Phawinee Subsomwong, Department of Microbiology and Immunology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan.Search for more papers by this authorShamshul Ansari
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Present Address: Evariste Tshibangu-Kabamba, Department of Parasitology, Graduate School of Medicine, Osaka City University, Osaka, Japan; Yuichi Matsuo, Department of Biomedical Laboratory Sciences, Kumamoto University, Kumamoto, Japan; Shamshul Ansari, Department of Microbiology, Chitwan Medical College, Bharatpur, Nepal; Phawinee Subsomwong, Department of Microbiology and Immunology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan.Search for more papers by this authorDalla Doohan
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia
Search for more papers by this authorBui Hoang Phuc
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Search for more papers by this authorPhawinee Subsomwong
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Present Address: Evariste Tshibangu-Kabamba, Department of Parasitology, Graduate School of Medicine, Osaka City University, Osaka, Japan; Yuichi Matsuo, Department of Biomedical Laboratory Sciences, Kumamoto University, Kumamoto, Japan; Shamshul Ansari, Department of Microbiology, Chitwan Medical College, Bharatpur, Nepal; Phawinee Subsomwong, Department of Microbiology and Immunology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan.Search for more papers by this authorLanggeng Agung Waskito
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia
Search for more papers by this authorTran Thanh Binh
Department of Endoscopy, Cho Ray Hospital, Ho Chi Minh City, Vietnam
Search for more papers by this authorLam Tung Nguyen
Department of Hepatogastroenterology, 108 Military Central Hospital, Hanoi, Vietnam
Search for more papers by this authorVu Van Khien
Department of Hepatogastroenterology, 108 Military Central Hospital, Hanoi, Vietnam
Search for more papers by this authorHo Dang Quy Dung
Department of Endoscopy, Cho Ray Hospital, Ho Chi Minh City, Vietnam
Search for more papers by this authorMuhammad Miftahussurur
Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia
Gastroentero-Hepatology Division, Department of Internal Medicine, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Airlangga University, Surabaya, Indonesia
Search for more papers by this authorAri Fahrial Syam
Division of Gastroenterology, Department of Intestinal Medicine, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
Search for more papers by this authorLotay Tshering
Department of Surgery, Jigme Dorji Wangchuck National Referral Hospital, Thimphu, Bhutan
Search for more papers by this authorRatha-korn Vilaichone
Gastroenterology Unit, Digestive Diseases Research Center, Thammasat University Hospital, Khlong Luang, Thailand
Department of Medicine, Chulabhorn International College of Medicine, Thammasat University Hospital, Khlong Luang, Thailand
Search for more papers by this authorVarocha Mahachai
GI and Liver Center, Bangkok Medical Center, Bangkok, Thailand
Search for more papers by this authorThawee Ratanachu-ek
Department of Surgery, Rajavithi Hospital, Bangkok, Thailand
Search for more papers by this authorPradeep Krishna Shrestha
Department of Gastroenterology, Maharajgunj Medical Campus, Tribhuvan University Teaching Hospital, Kathmandu, Nepal
Search for more papers by this authorThan Than Yee
Department of GI and HBP Surgery, No (2) Defense Service General Hospital, Nay Pyi Taw, Myanmar
Search for more papers by this authorKyaw Htet
Department of GI and HBP Surgery, No (1) Defense Service General Hospital, Mingaladon, Myanmar
Search for more papers by this authorHafeza Aftab
Department of Gastroenterology, Dhaka Medical Collage and Hospital, Dhaka, Bangladesh
Search for more papers by this authorTakeshi Matsuhisa
Department of Gastroenterology, Tama-Nagayama University Hospital, Nippon Medical School, Tama, Japan
Search for more papers by this authorTomohisa Uchida
Department of Molecular Pathology, Faculty of Medicine, Oita University, Yufu, Japan
Search for more papers by this authorTadayoshi Okimoto
Department of Gastroenterology, Faculty of Medicine, Oita University, Yufu, Japan
Search for more papers by this authorKazuhiro Mizukami
Department of Gastroenterology, Faculty of Medicine, Oita University, Yufu, Japan
Search for more papers by this authorMasaaki Kodama
Department of Gastroenterology, Faculty of Medicine, Oita University, Yufu, Japan
Faculty of Welfare and Health Science, Oita University, Yufu, Japan
Search for more papers by this authorKazunari Murakami
Department of Gastroenterology, Faculty of Medicine, Oita University, Yufu, Japan
Search for more papers by this authorNaohiko Takahashi
Clinical Laboratory Center, Oita University Hospital, Yufu, Japan
Search for more papers by this authorCorresponding Author
Yoshio Yamaoka
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan
Global Oita Medical Advanced Research Center for Health, Oita University, Yufu, Japan
Department of Medicine, Gastroenterology and Hepatology Section, Baylor College of Medicine, Houston, Texas, USA
Correspondence
Yoshio Yamaoka, Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan.
Email: [email protected]
Search for more papers by this authorDeclaration of conflict of interest: The authors declare that they have no conflict of interest.
Financial support: This work was supported by a grant from Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (15H02657, 16H05191, 16H06279, 18KK0266, and 19H03473) to Y. Y. and (17K09353) to J. A. This study was also supported by the Bualuang ASEAN Chair Professorship at Thammasat University, Thailand.
Abstract
Background and Aim
To determine the application range of diagnostic kits utilizing anti-Helicobacter pylori antibody, we tested a newly developed latex aggregation turbidity assay (latex) and a conventional enzyme-linked immunosorbent assay (E-plate), both containing Japanese H. pylori protein lysates as antigens, using sera from seven Asian countries.
Methods
Serum samples (1797) were obtained, and standard H. pylori infection status and atrophy status were determined by culture and histology (immunohistochemistry) using gastric biopsy samples from the same individuals. The two tests (enzyme-linked immunosorbent assay and latex) were applied, and receiver operating characteristics analysis was performed.
Results
Area under the curve (AUC) from the receiver operating characteristic of E-plate and latex curves were almost the same and the highest in Vietnam. The latex AUC was slightly lower than the E-plate AUC in other countries, and the difference became statistically significant in Myanmar and then Bangladesh as the lowest. To consider past infection cases, atrophy was additionally evaluated. Most of the AUCs decreased using this atrophy-evaluated status; however, the difference between the two kits was not significant in each country, but the latex AUC was better using all samples. Practical cut-off values were 3.0 U/mL in the E-test and 3.5 U/mL in the latex test, to avoid missing gastric cancer patients to the greatest extent possible.
Conclusions
The kits were applicable in all countries, but new kits using regional H. pylori strains are recommended for Myanmar and Bangladesh. Use of a cut-off value lower than the best cut-off value is essential for screening gastric cancer patients.
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