Phagomagnetic separation in combination with real-time PCR assay for detection of Salmonella Enteritidis and Typhimurium in dairy products
Yifeng Ding
Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, China
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China
Search for more papers by this authorZhiwei Li
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China
Search for more papers by this authorChenxi Huang
Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, China
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China
Search for more papers by this authorYiming Zhang
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China
Search for more papers by this authorJia Wang
Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, China
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China
Search for more papers by this authorCorresponding Author
Xiaohong Wang
Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, China
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China
Correspondence
Xiaohong Wang, Key Laboratory of Environment Correlative Dietology and College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Email: [email protected]
Search for more papers by this authorYifeng Ding
Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, China
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China
Search for more papers by this authorZhiwei Li
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China
Search for more papers by this authorChenxi Huang
Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, China
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China
Search for more papers by this authorYiming Zhang
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China
Search for more papers by this authorJia Wang
Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, China
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China
Search for more papers by this authorCorresponding Author
Xiaohong Wang
Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, China
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China
Correspondence
Xiaohong Wang, Key Laboratory of Environment Correlative Dietology and College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Email: [email protected]
Search for more papers by this authorAbstract
Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST) are the most commonly reported serotypes detected for the occurrence of Salmonellosis through foodborne transmission in recent years. In this study, a phagomagnetic separation in combination with qPCR (PhMS-qPCR) based on phage T102 as a recognition element was developed for rapid enrichment and detection of Salmonella in dairy products. Phage T102 was coated onto magnetic beads for capturing Salmonella from samples through magnetic separation, with a capture efficiency of ~90% demonstrated. During the subsequent qPCR process, captured Salmonella was identified by detecting the OmpC gene, and specific sdf and STM4495 genes to further distinguish the serotypes of Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST), respectively. The whole procedure can be performed in 2.5 h with a low detection limit of 10 CFU/mL in PBS. Subsequently, this detection strategy was successfully applied for the detection of Salmonella and serotype identification in spiked milk samples. This cell separation and detection system offers a promising alternative for rapid and accurate detection of Salmonella or Salmonella spp.
CONFLICT OF INTEREST STATEMENT
The authors have declared no conflict of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
| Filename | Description |
|---|---|
| jfs13091-sup-0001-supinfo.docxWord 2007 document , 28.2 KB | Table S1. Comparison of integrated PhMS-qPCR method with other methods for detection of pathogenic bacteria in food matrices. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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