Germ cell nuclear factor directly represses the transcription of peroxisome proliferator-activated receptor delta gene
Chengqiang He
College of Life Science, Shandong Normal University, Jinan 250014, China
Search for more papers by this authorCorresponding Author
Naizheng Ding
School of Biological Science and Technology, Central South University, Changsha 410012, China
College of Life Science, Peking University, Beijing 100871, China
*Corresponding author: Tel, 86-731-2650230; E-mail, [email protected]Search for more papers by this authorJie Kang
Department of Biology, Shangqiu Normal University, Shangqiu 476000, China
Search for more papers by this authorChengqiang He
College of Life Science, Shandong Normal University, Jinan 250014, China
Search for more papers by this authorCorresponding Author
Naizheng Ding
School of Biological Science and Technology, Central South University, Changsha 410012, China
College of Life Science, Peking University, Beijing 100871, China
*Corresponding author: Tel, 86-731-2650230; E-mail, [email protected]Search for more papers by this authorJie Kang
Department of Biology, Shangqiu Normal University, Shangqiu 476000, China
Search for more papers by this authorThis work was supported by grants from the National Natural Science Foundation of China (No. 30400039) and the Chinese Postdoctoral Foundation (No. 2003033069 and 2004036001)
Abstract
Germ cell nuclear factor (GCNF) is a transcription factor that can repress gene transcription and plays an important role during spermatogenesis. Peroxisome proliferator-activated receptor delta (PPARδ) is a nuclear hormone receptor belonging to the steroid receptor superfamily. It can activate the expression of many genes, including those involved in lipid metabolism. In this report, we showed that GCNF specifically interacts with PPARδ promoter. Overexpression of GCNF in African green monkey SV40-transformed kidney fibroblast COS7 cells and mouse embryo fibroblast NIH 3T3 cells represses the activity of PPARδ promoter. The mutation of GCNF response element inPPARδ promoter relieves the repression in NIH 3T3 cells and mouse testis. Moreover, we showed that GCNF in nuclear extracts of mouse testis is able to bind to PPARδ promoter directly. We also found that GCNFand PPARδ mRNA were expressed with different patterns in mouse testis by in situ hybridization. These results suggested that GCNF might be a negative regulator of PPARδ gene expression through its direct interaction with PPARδ promoter in mouse testis.
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