RAPID RECOVERY OF DNA FROM AGAROSE GEL SLICE USING A MICROWAVE*
*
The first two authors have made equal contributions to this work.
ABSTRACT
A method for the rapid recovery of DNA from agarose gel slices for polymerase chain reaction (PCR) applications was described. DNA bands were excised from agarose gel and diluted with distilled water. One-minute microwave treatment at full power was performed, immediately followed by 1-min centrifugation. The supernatant thus recovered can be directly used in PCR amplifications with minimal delay. This protocol represents an ultrafast and inexpensive alternative to commercial kits.
