Effects of Losartan, HO-1 Inducers or HO-1 Inhibitors on Erectile Signaling in Diabetic Rats
Mohamed Talaat Abdel Aziz MD
Faculty of Medicine, Cairo University, Medical Biochemistry, Unit of Biochemistry & Molecular Biology, Cairo, Egypt;
Search for more papers by this authorMohamed Farid El Asmer MD
Faculty of Medicine, Ain Shams University, Medical Biochemistry Department, Cairo, Egypt;
Search for more papers by this authorTaymour Mostafa MD
Faculty of Medicine, Cairo University, Andrology Department, Cairo, Egypt;
Search for more papers by this authorHazem Atta MD
Faculty of Medicine, Cairo University, Medical Biochemistry, Unit of Biochemistry & Molecular Biology, Cairo, Egypt;
Search for more papers by this authorSoheir Mahfouz MD
Faculty of Medicine, Cairo University, Pathology Department, Cairo, Egypt;
Search for more papers by this authorCorresponding Author
Hanan Fouad MD
Faculty of Medicine, Cairo University, Medical Biochemistry, Unit of Biochemistry & Molecular Biology, Cairo, Egypt;
Hanan Fouad, MD, Medical Biochemistry Department, Faculty of Medicine, Cairo University, POB 11562, Cairo, Egypt. Tel: +202-23632297; Fax: +202-23632297; E-mail: [email protected]Search for more papers by this authorLaila Rashed MD
Faculty of Medicine, Cairo University, Medical Biochemistry, Unit of Biochemistry & Molecular Biology, Cairo, Egypt;
Search for more papers by this authorDina Sabry MD
Faculty of Medicine, Cairo University, Medical Biochemistry, Unit of Biochemistry & Molecular Biology, Cairo, Egypt;
Search for more papers by this authorAmira Hassouna MD
Faculty of Medicine, Cairo University, Medical Biochemistry, Unit of Biochemistry & Molecular Biology, Cairo, Egypt;
Search for more papers by this authorAhmed Talaat Abdel Aziz MD
Faculty of Medicine, Cairo University, Medical Biochemistry, Unit of Biochemistry & Molecular Biology, Cairo, Egypt;
Search for more papers by this authorAmira Senbel MD
Faculty of Pharmacy, Alexandria University, Pharmacology Department, Alexandria, Egypt;
Search for more papers by this authorAhmed Demery MD
Faculty of Medicine, Six October University, Medical Biochemistry Department, Cairo, Egypt
Search for more papers by this authorMohamed Talaat Abdel Aziz MD
Faculty of Medicine, Cairo University, Medical Biochemistry, Unit of Biochemistry & Molecular Biology, Cairo, Egypt;
Search for more papers by this authorMohamed Farid El Asmer MD
Faculty of Medicine, Ain Shams University, Medical Biochemistry Department, Cairo, Egypt;
Search for more papers by this authorTaymour Mostafa MD
Faculty of Medicine, Cairo University, Andrology Department, Cairo, Egypt;
Search for more papers by this authorHazem Atta MD
Faculty of Medicine, Cairo University, Medical Biochemistry, Unit of Biochemistry & Molecular Biology, Cairo, Egypt;
Search for more papers by this authorSoheir Mahfouz MD
Faculty of Medicine, Cairo University, Pathology Department, Cairo, Egypt;
Search for more papers by this authorCorresponding Author
Hanan Fouad MD
Faculty of Medicine, Cairo University, Medical Biochemistry, Unit of Biochemistry & Molecular Biology, Cairo, Egypt;
Hanan Fouad, MD, Medical Biochemistry Department, Faculty of Medicine, Cairo University, POB 11562, Cairo, Egypt. Tel: +202-23632297; Fax: +202-23632297; E-mail: [email protected]Search for more papers by this authorLaila Rashed MD
Faculty of Medicine, Cairo University, Medical Biochemistry, Unit of Biochemistry & Molecular Biology, Cairo, Egypt;
Search for more papers by this authorDina Sabry MD
Faculty of Medicine, Cairo University, Medical Biochemistry, Unit of Biochemistry & Molecular Biology, Cairo, Egypt;
Search for more papers by this authorAmira Hassouna MD
Faculty of Medicine, Cairo University, Medical Biochemistry, Unit of Biochemistry & Molecular Biology, Cairo, Egypt;
Search for more papers by this authorAhmed Talaat Abdel Aziz MD
Faculty of Medicine, Cairo University, Medical Biochemistry, Unit of Biochemistry & Molecular Biology, Cairo, Egypt;
Search for more papers by this authorAmira Senbel MD
Faculty of Pharmacy, Alexandria University, Pharmacology Department, Alexandria, Egypt;
Search for more papers by this authorAhmed Demery MD
Faculty of Medicine, Six October University, Medical Biochemistry Department, Cairo, Egypt
Search for more papers by this authorABSTRACT
Introduction. Activation of the renin-angiotensin system which is common in diabetes mellitus might affect heme oxygenase (HO-1) gene expression.
Aim. Assessment of the effects of administration of angiotensin II (Ang II) receptor antagonist (losartan) with HO-1 inducer or inhibitor on erectile signaling in diabetic rats.
Materials and Methods. Seventy male rats were divided equally into seven groups; healthy controls, streptozotocin-induced diabetic rats, rats on citrate buffer, diabetic rats on losartan, diabetic rats on HO-1 inducer (cobalt protoporphyrin [CoPP]), diabetic rats on losartan and CoPP, and diabetic rats on losartan and HO-1 inhibitor (stannus mesoporphyrin [SnMP]).
Main Outcome Measure. HO enzyme activity, HO-1 gene expression, cyclic guanosine monophosphate (cGMP) assay, intracavernosal pressure (ICP), and cavernous tissue sinusoids surface area.
Results. HO-1 gene expression, HO enzymatic activity, and cGMP were significantly decreased in the cavernous tissue of diabetic rats. These parameters were significantly elevated with the use of CoPP that restored the normal control levels of HO enzyme activity. Administration of losartan exhibited a significant enhancing effect on these parameters compared with the diabetic group, but not restored to the control levels, whereas administration of CoPP combined with losartan led to the restoration of their normal levels. ICP demonstrated significant decline in diabetic rats. The use of CoPP and/or losartan led to its significant improvement compared with diabetic rats. Administration of either losartan and/or CoPP led to a significant increase in the cavernous sinusoids surface area of diabetic rats. Administration of losartan with SnMP significantly decreased the enhancing effect of losartan on the studied parameters.
Conclusion. The decline in erectile function in diabetes mellitus could be attributed to the downregulation of HO-1 gene expression. HO-1 induction added to Ang II receptor antagonist could improve erectile function. Abdel Aziz MT, El Asmer MF, Mostafa T, Atta H, Mahfouz S, Fouad H, Rashed L, Sabry D, Hassouna A, Abdel Aziz AT, Senbel A, and Demery A. Effects of losartan, HO-1 inducers or HO-1 inhibitors on erectile signaling in diabetic rats. J Sex Med 2009;6:3254–3264.
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