Thermography-measured effect of capsaicin, methylprednisolone and mitomycin on the survival of random skin flaps in rats

Dražen Shejbal

School of Medicine, Division of Otorhinolaryngology, University of Zagreb, Pakrac County Hospital, Pakrac, Croatia

Petar Drvis

School of Medicine, Department of Otorhinolaryngology and Head and Neck Surgery, University of Zagreb, Sestre Milosrdnice University Hospital, Zagreb, Croatia

Search for more papers by this author

Vladimir Bedekovic,

Vladimir Bedekovic

School of Medicine, Department of Otorhinolaryngology and Head and Neck Surgery, University of Zagreb, Sestre Milosrdnice University Hospital, Zagreb, Croatia

Search for more papers by this author

Dražen Shejbal,

Dražen Shejbal

School of Medicine, Division of Otorhinolaryngology, University of Zagreb, Pakrac County Hospital, Pakrac, Croatia

Search for more papers by this author

Petar Drvis,

Petar Drvis

School of Medicine, Department of Otorhinolaryngology and Head and Neck Surgery, University of Zagreb, Sestre Milosrdnice University Hospital, Zagreb, Croatia

Search for more papers by this author

Vladimir Bedekovic,

Vladimir Bedekovic

School of Medicine, Department of Otorhinolaryngology and Head and Neck Surgery, University of Zagreb, Sestre Milosrdnice University Hospital, Zagreb, Croatia

Search for more papers by this author

First published: 06 May 2011

https://doi.org/10.1111/j.1600-0846.2011.00547.x

Citations: 5

Address:
Dražen Shejbal
Division of Otorhinolaryngology
Pakrac County Hospital
Bolnička 68
34550 Pakrac
Croatia
Tel: +385 911 515 929
Fax: +38 543 333 091
e-mail:[email protected]

Share a link

Email
Wechat
Bluesky

Abstract

Aims: This study was designed to determine if steroids, capsaicin and mytomicin improved skin flap survival in rats, and it has confirmed that thermography is an effective method to measured skin flap vitality.

Methods: Forty female Wistar rats were randomised into four groups. A standardised full thickness inferiorly based dorsal random-pattern skin flap was raised, and a gelatine sponge was placed before suturing between the flap and its recipient bed, soaked with 0.9 % saline in the control group and with capsaicin, methylprednisolone and mitomycini in the experimental groups. Vitality of the flap and of the survival area was measured by infrared thermography.

Results: Methylprednisolone statistically significant decrease skin flap necrosis, approximately 56%, when compared with controls (P< 0.05).

Conclusion: Corticosteroids applied subcutaneously in a single dose on a gelatine sponge produced a statistically significant improvement of the survival of random skin flaps in rats, whereas capsaicin and mitomycin showed no improvement.

References

1DavisRE, WachholzJH, JassirD, PerlynCA, AgramaMH.Comparison of topical anti-ischemic agents in the salvage of failing random-pattern skin flaps in rats. Arch Facial Plast Surg1999; 1: 27–32.
10.1001/archfaci.1.1.27
CAS PubMed Google Scholar
2HuemerGM, WechselbergerG, Otto-SchoellerA, GurunluogluR, Piza-KatzerH, SchoellerT.Improved dorsal random-pattern skin flap survival in rats with a topically applied combination of nonivamide and nicoboxil. Plast Reconstr Surg2003; 111: 1207–1211.
10.1097/01.PRS.0000047404.28025.C5
PubMed Web of Science® Google Scholar
3KaracaoglanN, AkbasH.Effect of parenteral pentoxifylline and topical nitroglycerin on skin flap survival. Otolaryngol Head Neck Surg1999; 120: 272–274.
10.1016/S0194-5998(99)70419-5
CAS PubMed Web of Science® Google Scholar
4SmithDK, DolanRW.Effects of vasoactive topical agents on the survival of dorsal skin flaps in rats. Otolaryngol Head Neck Surg1999; 121: 220–225.
10.1016/S0194-5998(99)70175-0
CAS PubMed Web of Science® Google Scholar
5BuemiM, VaccaroM, SturialeAet al. Recombinant human erythropoietin influences revascularisation and healing in a rat model of random ischaemic flaps. Acta Derm Venerol2002; 82: 411–417.
10.1080/000155502762064520
CAS PubMed Web of Science® Google Scholar
6KrygerZ, ZhangF, DoganT, ChengC, LineaweaverWC, BunckeHJ.The effects of VEGF on survival of a random flap in the rat: examination of various routes of administration. Br J Plast Surg 2000; 53: 234–239.
CAS PubMed Web of Science® Google Scholar
7SalernoGM, McBrideDM, BleicherJN, WatsonP, StrombergBV.Ciprostene and indomethacin partially reverse the mechanisms of distal end necrosis in the rat random skin flap. Ann Plast Surg1992; 28: 526–534.
10.1097/00000637-199206000-00005
CAS PubMed Web of Science® Google Scholar
8WangBH, YeC, StaggCA, LinM, FawcettT, Van der KolkCA, UdelsmanR.Improved free musculocutaneous flap survival with induction of heat shock protein. Plast Reconstr Surg1998; 101: 776–784.
10.1097/00006534-199803000-00029
CAS PubMed Web of Science® Google Scholar
9DasS, KumarKN.Nitric oxide: its identity and role in blood pressure control. Life Sci 1995; 57: 1547–1556.
CAS PubMed Web of Science® Google Scholar
10KuoPS, SchroederRA.The emerging multifaceted roles of nitric oxide. Ann Surg1995; 221: 220–235.
10.1097/00000658-199503000-00003
CAS PubMed Web of Science® Google Scholar
11KubesP, SuzukiM, GrangerDN.Nitric oxide in inflammation and immune response. Ann Med1995; 27: 359–367.
10.3109/07853899509002589
PubMed Web of Science® Google Scholar
12RandomskiMW, MoncadaS.The biological and pharmacological role of nitric oxide in platelet function. Adv Exp Med Biol1993; 334: 251–264.
10.1007/978-1-4615-2994-1_20
Google Scholar
13UmansJG, LeviR.Nitric oxide in the regulation of blood flow and arterial pressure. Annu Rev Physiol1995; 57: 771–790.
10.1146/annurev.ph.57.030195.004011
CAS PubMed Web of Science® Google Scholar
14DudekRR, WildhirtS, PintoV, GeislerG, BingRJ.Dexamethasone inhibits the expression of an inducible nitric oxide synthase in infarcted rabbit myocardium. Biochem Biophys Res Commun1994; 87: 10043–10047.
Google Scholar
15GribbeO, LundebergT, SamuelsonUE, WiklundPN.Dexamethasone increased survival and attenuates induction of inducible nitric oxide synthase in experimental skin flaps. Ann Plast Surg1999; 42: 180–184.
10.1097/00000637-199902000-00013
CAS PubMed Web of Science® Google Scholar
16BuckSH, BurksFT.The neuropharmacology of capsaicin: review of some recent observations. Pharmacol Rev 1986; 3: 216–220.
Web of Science® Google Scholar
17ChangCC, MaruyamaY, InomataK.Increased flap survival after sensory denervation with capsaicin accompanied by delay. J Reconstr Microsurg1990; 6: 29–33.
10.1055/s-2007-1006799
CAS PubMed Web of Science® Google Scholar
18IinumaT, SawadaY.Topical application of capsaicin and flap survival. Br J Plast Surg1996; 49: 319–320.
10.1016/S0007-1226(96)90163-0
CAS PubMed Web of Science® Google Scholar
19MiawakiT, JacksonIT, BierUC, AndrusL, WilliamsF, BradfordM.The effect of capsaicin ointment on skin for the survival of a cutaneous flap. Eur J Plast Surg2001; 24: 28–30.
10.1007/s002380000221
Web of Science® Google Scholar
20SawadaY, YotsuyanagiT, YokoiK, IshitaK.Plaster containing capsaicin increases the survival of experimental flaps: effects of post and preoperative administration. Eur J Plast Surg 1997; 20: 256–259.
Web of Science® Google Scholar
21WestinM, HedenP.Sensory denervation with capsaicin does not influence the critical ischemia time in rat island flaps. Scand J Plast Reconstr Surg Hand Surg1998; 23: 173–176.
10.3109/02844318909075113
Google Scholar
22DoolinEJ, StrandeLF, TsunoK, SantosMC.Pharmacologic inhibition of collagen in an experimental model of subglottic stenosis. Ann Otol Rhinol Laryngol1998; 107: 275–279.
10.1177/000348949810700402
CAS PubMed Web of Science® Google Scholar
23GhabnerBA, AllegraCJ, CurtGA, CalabresiP. Antineoplastic agents. In: LS Goodman, A Gilman, JG Harman, LE Limbrid, eds. The pharmacological basis of therapeutics. New York: McGraw-Hill, 1996: 1233–1287.
Google Scholar
24BorasI, DrvišP, MuftićO, ShejbalD, SušaM, ŠvaićS. Numerical modeling for heat conduction in human tissue by means of thermography. In: B. Mijoviced. Ergonomics, 2nd International Ergonomics Conference, Croatian ergonomics society, Zagreb, Croatia. 2004: 51–58.
Google Scholar
25EisenbeisW, MarotzJ, SchradeJP.Reflection-optical multispectral imaging method for objective determination of burn depth. Burns1999; 25: 697–704.
10.1016/S0305-4179(99)00078-9
PubMed Web of Science® Google Scholar
26PariskyYR, SardiA, HammR, HughesK, EssermanL, RustS, CallahanK.Efficacy of computerized infrared imaging analysis to evaluate mammographically suspicious lesions. AJR Am J Roentgenol2003; 180: 263–269.
10.2214/ajr.180.1.1800263
CAS PubMed Web of Science® Google Scholar
27PauHW, SievertU, WildW.Thermography of the skin covering a cochlear implant – temperature as an indicator for blood circulation. Laryngorhinootologie2003; 82: 615–619.
CAS PubMed Web of Science® Google Scholar
28DrvisP, ShejbalD, SvaicS, BorasI, SundovI, SusaM, BedekovicV, KalogjeraL, PetrovicI, SikiricP. Evaluation of cutaneous flap survival by IR thermography. In: JM Buchlined. QUIRT, Proceedings of 7th International Conference on Quantitative Infrared Thermography, Von Karman Institute for Fluid Dynamics, Brissel, Belgium.2004: J.4.1–J.4.9.
10.21611/qirt.2004.047
Google Scholar
29IgotaS, YamamotoN.Making of a facial perforator map by thermography. Ann Plast Surg2000; 44: 596–600.
10.1097/00000637-200044060-00003
PubMed Web of Science® Google Scholar
30KuruB, DincS, CamlibelM, DurmusE, AlagölH.Efficacy of postoperative steroids on ischemic skin flap survival in rats. Eur J Plast Surg2003; 26: 79–81.
10.1007/s00238-003-0484-y
Google Scholar
31BrewsterD, CuppC, WesterDC.The effects of macrophages on the survival of random skin flaps in swine. Laryngoscope1996; 106: 1094–1098.
10.1097/00005537-199609000-00010
CAS PubMed Web of Science® Google Scholar
32MoilianenE, VapaataloH.Nitric oxide in inflammation and immune response. Ann Med1995; 27: 359–367.
10.3109/07853899509002589
PubMed Web of Science® Google Scholar
33WinkDA, HanbauerI, GrishamMBet al. Chemical biology of nitric oxide: regulation and protective and toxic mechanisms. Curr Top Cell Regul 1996; 34: 159–187.
CAS PubMed Web of Science® Google Scholar

Citing Literature

Volume18, Issue2

May 2012

Pages 157-161

Thermography-measured effect of capsaicin, methylprednisolone and mitomycin on the survival of random skin flaps in rats

Abstract

References

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

Thermography-measured effect of capsaicin, methylprednisolone and mitomycin on the survival of random skin flaps in rats

Abstract

References

Citing Literature

References

Related

Information