VIM and IMP metallo-β-lactamases and other extended-spectrum β-lactamases in Escherichia coli and Klebsiella pneumoniae from environmental samples in a Tunisian hospital

CHEDLY CHOUCHANI

Université de Carthage, Institut Supérieur des Sciences et Technologies de l’Environnement de Borj-Cedria, Technopôle de Borj-Cedria, Hammam-Lif, Tunisie

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RIM MARRAKCHI,

RIM MARRAKCHI

Université de Carthage, Institut Supérieur des Sciences et Technologies de l’Environnement de Borj-Cedria, Technopôle de Borj-Cedria, Hammam-Lif, Tunisie

Laboratoire de Microbiologie et Molécules Bioactives, Faculté des Sciences de Tunis, Campus Universitaire, El-Manar II

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LEILA FERCHICHI,

LEILA FERCHICHI

Laboratoire de Bactériologie, Hôpital Régionale de Kasserine, Kasserine, Tunisie

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ALLAAEDDIN EL SALABI,

ALLAAEDDIN EL SALABI

Department of Infection, Immunity & Biochemistry, School of Medicine, Cardiff University, Cardiff, UK

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TIMOTHY R. WALSH,

TIMOTHY R. WALSH

Department of Infection, Immunity & Biochemistry, School of Medicine, Cardiff University, Cardiff, UK

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CHEDLY CHOUCHANI,

CHEDLY CHOUCHANI

Université de Carthage, Institut Supérieur des Sciences et Technologies de l’Environnement de Borj-Cedria, Technopôle de Borj-Cedria, Hammam-Lif, Tunisie

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RIM MARRAKCHI,

RIM MARRAKCHI

Université de Carthage, Institut Supérieur des Sciences et Technologies de l’Environnement de Borj-Cedria, Technopôle de Borj-Cedria, Hammam-Lif, Tunisie

Laboratoire de Microbiologie et Molécules Bioactives, Faculté des Sciences de Tunis, Campus Universitaire, El-Manar II

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LEILA FERCHICHI,

LEILA FERCHICHI

Laboratoire de Bactériologie, Hôpital Régionale de Kasserine, Kasserine, Tunisie

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ALLAAEDDIN EL SALABI,

ALLAAEDDIN EL SALABI

Department of Infection, Immunity & Biochemistry, School of Medicine, Cardiff University, Cardiff, UK

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TIMOTHY R. WALSH,

TIMOTHY R. WALSH

Department of Infection, Immunity & Biochemistry, School of Medicine, Cardiff University, Cardiff, UK

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First published: 21 July 2011

https://doi.org/10.1111/j.1600-0463.2011.02793.x

Citations: 25

Dr Chedly Chouchani, Université de Carthage, Institut Supérieur des Sciences et Technologies de l’Environnement de Borj-Cedria, Technopôle de Borj-Cedria, BP-1003, Hammam-Lif 2050, Tunisie. e-mail: [email protected]

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Abstract

Chouchani C, Marrakchi R, Ferchichi L, El Salabi A, Walsh TR. VIM and IMP metallo-β-lactamases and other extended-spectrum β-lactamases in Escherichia coli and Klebsiella pneumoniae from environmental samples in a Tunisian hospital. APMIS 2011; 119: 725–32.

An extremely drug-resistant Enterobacteriaceae species emerged in Kasserine Hospital, Tunisia between 2009 and 2010 causing a local outbreak. We aimed to characterize extended-spectrum β-lactamase (ESBL) and metallo-β-lactamase (MBL)-producing Enterobacteriaceae from the hospital environment. Swabs were collected from ten different wards from Kasserine Hospital, Tunisia. A total of 46 isolates were cultured onto MacConkey agar supplemented with ceftazidime to select for ESBL-producing Enterobacteriaceae. Identification and susceptibility patterns were performed using Phoenix-automated phenotypic identification criteria. Extended spectrum β-lactamases (ESBLs) were detected using cefepime ESBL E-test. Colony blotting was first used to detect the occurrence of bla_SHV, bla_CTX-M, bla_CMY, bla_IMP, and bla_VIM genes. PCR was used to amplify these genes, and the amplicons were sequenced and analyzed. Total DNA was digested with XbaI, and PFGE was used to type the major isolates that produced IMP-1. Among the 46 isolates, 63% were Klebsiella pneumoniae, 13% were Escherichia coli, 8.7% were Proteus mirabilis, 6% were Enterobacter cloaceae, 4.3% were Providencia rettgeri, 2.5% were Serratia marcescens, and 2.5% were Pantoea agglomerans. PCR amplification and DNA sequencing showed that hospital environment isolates produced SHV-125, CTX-M-15, CMY-2 ESBLs, and IMP-1 and VIM-2 MBLs. PFGE typing showed the emergence of IMP-1 MBL-producing K. pneumoniae isolates that were not clonal. In this study, we report the first characterization of IMP-1 and VIM-2 MBL-producing K. pneumoniae and E. coli isolates collected from Kasserine Hospital, Tunisia.

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Volume119, Issue10

October 2011

Pages 725-732

VIM and IMP metallo-β-lactamases and other extended-spectrum β-lactamases in Escherichia coli and Klebsiella pneumoniae from environmental samples in a Tunisian hospital

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VIM and IMP metallo-β-lactamases and other extended-spectrum β-lactamases in Escherichia coli and Klebsiella pneumoniae from environmental samples in a Tunisian hospital

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