Volume 119, Issue 10 pp. 710-719

Secretory IgA–Fcα receptor interaction modulating phagocytosis and microbicidal activity by phagocytes in human colostrum of diabetics

EDUARDO LUZíA FRANçA

EDUARDO LUZíA FRANçA

Post Graduate Program in Immunology and Parasitology of the Institute of Biological and Health Sciences, Federal University of Mato Grosso, Barra do Garças, MT

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GLILCIANE MORCELI

GLILCIANE MORCELI

Post Graduate Program in Gynecology, Obstetrics and Mastology of the Botucatu Medical School, Unesp, Botucatu, SP

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DANNY LAURA GOMES FAGUNDES

DANNY LAURA GOMES FAGUNDES

Post Graduate Program in Material Science of the Federal University of Mato Grosso, Barra do Garças, MT, Brazil

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MARILZA VIEIRA CUNHA RUDGE

MARILZA VIEIRA CUNHA RUDGE

Post Graduate Program in Gynecology, Obstetrics and Mastology of the Botucatu Medical School, Unesp, Botucatu, SP

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IRACEMA DE MATTOS PARANHOS CALDERON

IRACEMA DE MATTOS PARANHOS CALDERON

Post Graduate Program in Immunology and Parasitology of the Institute of Biological and Health Sciences, Federal University of Mato Grosso, Barra do Garças, MT

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ADENILDA CRISTINA HONORIO-FRANçA

ADENILDA CRISTINA HONORIO-FRANçA

Post Graduate Program in Immunology and Parasitology of the Institute of Biological and Health Sciences, Federal University of Mato Grosso, Barra do Garças, MT

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First published: 18 July 2011
Citations: 25
Adenilda Cristina Honorio França, Institute of Biological and Health Sciences, Federal University of Mato Grosso, Rodovia BR 070, Km 5 s/no, CEP: 78600-000, Barra do Garças, MT. Brazil. e-mail: [email protected]

Abstract

França EL, Morceli G, Fagundes DLG, Rudge MVC, Calderon I de MP, Honorio-França AC. Secretory IgA–Fcα receptor interaction modulating phagocytosis and microbicidal activity by phagocytes in human colostrum of diabetics. APMIS 2011; 119: 710–19.

The effects of secretory immunoglobulin A (SIgA) interaction with its specific Fcα receptors on colostral phagocytes needs further investigation, especially with respect to diabetic women. Accordingly, we studied the colostrum of hyperglycemic women to assess SIgA interactions with Fcα receptors of macrophages as well as the functional activity of these cells. The women were divided for colostrum sampling according to their glycemic status: normoglycemia (N = 51), mild hyperglycemia (N = 23), and diabetes (N = 25) groups. We determined the FcαR expression, the IgA on the surface and the surface-bound IgA in colostrum macrophages. We also evaluated the superoxide release and bactericidal killing of these cells. Colostral phagocytes expressed FcαR, contained IgA on the surface and are able to bind to purified SIgA. The bactericidal activity of colostral phagocytes from the hyperglycemic women was similar to that of normoglycemic only when SIgA was used as opsonin. Addition of a MoAb anti-human Fcα receptor resulted in a significant decrease of superoxide release and bacterial killing by macrophages when bacteria were opsonized with purified SIgA, suggesting an interaction between SIgA and FcαR. The stimulatory effects of SIgA on the functional activity of phagocytes therefore protect infants, especially of diabetic women, against intestinal infections.

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