Construction of bacterial artificial chromosome library from electrochemical microorganisms
Jung Ho Back
Biomedical Research Center, Korea Institute of Science and Technology, P. O. Box 131, Cheongryang, Seoul, Korea
The Graduate School of Biotechnology, Korea University, Seoul, Korea
Search for more papers by this authorMan Su Kim
Biomedical Research Center, Korea Institute of Science and Technology, P. O. Box 131, Cheongryang, Seoul, Korea
The Graduate School of Biotechnology, Korea University, Seoul, Korea
Search for more papers by this authorHyuk Cho
Biomedical Research Center, Korea Institute of Science and Technology, P. O. Box 131, Cheongryang, Seoul, Korea
Search for more papers by this authorIn Seop Chang
The Graduate School of Biotechnology, Korea University, Seoul, Korea
Search for more papers by this authorJiyoung Lee
The Graduate School of Biotechnology, Korea University, Seoul, Korea
Water Environment and Remediation Research Center, Korea Institute of Science and Technology, 39-1 Hawalkok-dong, Sungbuk-ku, Seoul 136-791, South Korea
Search for more papers by this authorKyung Sik Kim
The Graduate School of Biotechnology, Korea University, Seoul, Korea
Water Environment and Remediation Research Center, Korea Institute of Science and Technology, 39-1 Hawalkok-dong, Sungbuk-ku, Seoul 136-791, South Korea
Search for more papers by this authorByung Hong Kim
Water Environment and Remediation Research Center, Korea Institute of Science and Technology, 39-1 Hawalkok-dong, Sungbuk-ku, Seoul 136-791, South Korea
Search for more papers by this authorYoung In Park
The Graduate School of Biotechnology, Korea University, Seoul, Korea
Search for more papers by this authorCorresponding Author
Ye Sun Han
Department of Advanced Fusion Technology, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea
*Corresponding author. Tel.: +82-2-958-5933; fax: +82-2-958-5909, E-mail address: [email protected]Search for more papers by this authorJung Ho Back
Biomedical Research Center, Korea Institute of Science and Technology, P. O. Box 131, Cheongryang, Seoul, Korea
The Graduate School of Biotechnology, Korea University, Seoul, Korea
Search for more papers by this authorMan Su Kim
Biomedical Research Center, Korea Institute of Science and Technology, P. O. Box 131, Cheongryang, Seoul, Korea
The Graduate School of Biotechnology, Korea University, Seoul, Korea
Search for more papers by this authorHyuk Cho
Biomedical Research Center, Korea Institute of Science and Technology, P. O. Box 131, Cheongryang, Seoul, Korea
Search for more papers by this authorIn Seop Chang
The Graduate School of Biotechnology, Korea University, Seoul, Korea
Search for more papers by this authorJiyoung Lee
The Graduate School of Biotechnology, Korea University, Seoul, Korea
Water Environment and Remediation Research Center, Korea Institute of Science and Technology, 39-1 Hawalkok-dong, Sungbuk-ku, Seoul 136-791, South Korea
Search for more papers by this authorKyung Sik Kim
The Graduate School of Biotechnology, Korea University, Seoul, Korea
Water Environment and Remediation Research Center, Korea Institute of Science and Technology, 39-1 Hawalkok-dong, Sungbuk-ku, Seoul 136-791, South Korea
Search for more papers by this authorByung Hong Kim
Water Environment and Remediation Research Center, Korea Institute of Science and Technology, 39-1 Hawalkok-dong, Sungbuk-ku, Seoul 136-791, South Korea
Search for more papers by this authorYoung In Park
The Graduate School of Biotechnology, Korea University, Seoul, Korea
Search for more papers by this authorCorresponding Author
Ye Sun Han
Department of Advanced Fusion Technology, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea
*Corresponding author. Tel.: +82-2-958-5933; fax: +82-2-958-5909, E-mail address: [email protected]Search for more papers by this authorAbstract
A microbial fuel cell is a device that directly converts metabolic energy into electricity, using electrochemical technology. The analysis of large genome fragments recovered directly from microbial communities represents one promising approach to characterizing uncultivated electrochemical microorganisms. To further assess the utility of this approach, we constructed large-insert (140 kb) bacterial artificial chromosome (BAC) libraries from the genomic DNA of a microbial fuel cell, which had been operated for three weeks using acetate media. We screened the expression of several ferric reductase activities in the Escherichia coli host, in order to determine the extent of heterologous expression of metal-ion-reducing enzymes in the library. Phylogenetic analysis of 16S rRNA gene sequences recovered from the BAC libraries indicates that they contain DNA from a wide diversity of microbial organisms. The constructed bacterial library proved a powerful tool for exploring metal-ion reductase activities, providing information on the electron transport pathway of electrochemical microbial (ECM) organisms.
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