Protein candidates for the serodiagnosis of rickettsioses
Malgorzata Kowalczewska
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, URMITE, CNRS UMR 6236 – IRD 198, Faculté de Médecine, IFR48, Université de la Méditerranée, Marseille, France
Search for more papers by this authorManohari Vellaiswamy
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, URMITE, CNRS UMR 6236 – IRD 198, Faculté de Médecine, IFR48, Université de la Méditerranée, Marseille, France
Search for more papers by this authorClaude Nappez
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, URMITE, CNRS UMR 6236 – IRD 198, Faculté de Médecine, IFR48, Université de la Méditerranée, Marseille, France
Search for more papers by this authorRenaud Vincentelli
AFMB UMR 6098, CNRS, Universités Aix-Marseille I & II, Marseille, France
Search for more papers by this authorBernard La Scola
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, URMITE, CNRS UMR 6236 – IRD 198, Faculté de Médecine, IFR48, Université de la Méditerranée, Marseille, France
Search for more papers by this authorCorresponding Author
Didier Raoult
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, URMITE, CNRS UMR 6236 – IRD 198, Faculté de Médecine, IFR48, Université de la Méditerranée, Marseille, France
Correspondence: Didier Raoult, Unité des Rickettsies, IRD CNRS UMR 6236, Faculté de Médecine, 27, Bd Jean Moulin, 13385 Marseille, France. Tel.: +33 0 4 91324480; fax: +33 0 4 91387772; e-mail: [email protected]Search for more papers by this authorMalgorzata Kowalczewska
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, URMITE, CNRS UMR 6236 – IRD 198, Faculté de Médecine, IFR48, Université de la Méditerranée, Marseille, France
Search for more papers by this authorManohari Vellaiswamy
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, URMITE, CNRS UMR 6236 – IRD 198, Faculté de Médecine, IFR48, Université de la Méditerranée, Marseille, France
Search for more papers by this authorClaude Nappez
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, URMITE, CNRS UMR 6236 – IRD 198, Faculté de Médecine, IFR48, Université de la Méditerranée, Marseille, France
Search for more papers by this authorRenaud Vincentelli
AFMB UMR 6098, CNRS, Universités Aix-Marseille I & II, Marseille, France
Search for more papers by this authorBernard La Scola
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, URMITE, CNRS UMR 6236 – IRD 198, Faculté de Médecine, IFR48, Université de la Méditerranée, Marseille, France
Search for more papers by this authorCorresponding Author
Didier Raoult
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, URMITE, CNRS UMR 6236 – IRD 198, Faculté de Médecine, IFR48, Université de la Méditerranée, Marseille, France
Correspondence: Didier Raoult, Unité des Rickettsies, IRD CNRS UMR 6236, Faculté de Médecine, 27, Bd Jean Moulin, 13385 Marseille, France. Tel.: +33 0 4 91324480; fax: +33 0 4 91387772; e-mail: [email protected]Search for more papers by this authorAbstract
The laboratory diagnosis of rickettsioses is based on serology (reference method), cell culture and/or molecular tools. However, the main drawback of serology is its incapacity to provide identification of Rickettsiae at the level of species. The aim of this study was to propose the versatile protein markers able to discriminate the patients with murine typhus from those with Mediterranean spotted fever. We have cloned and expressed 20 proteins of Rickettsia prowazekii and Rickettsia rickettsii, respectively, using the GATEWAY approach. These recombinant proteins were screened by ELISA with sera of infected patients with Rickettsia typhi and Rickettsia conorii, respectively. We identified several potential markers which allowed infection due to R. typhi to be discriminated from those due to R. conorii. However, the values of test-operating parameters were not sufficient for its ‘routine’ clinical use. Our diagnostic test requires further optimization for be applied as a point-of-care strategy in the management of patients with suspected cases of rickettsiosis.
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