Correspondence: Pavel Švec, Czech Collection of Microorganisms, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic. Tel.: +420 549 497 601; fax: +420 543 247 339; e-mail: [email protected]

Editor: Kai Man Kam

Read the full text

About

PDF

Tools

Share a link

Email
Wechat
Bluesky

Abstract

Fifteen lactic acid bacterial strains were isolated from blood cultures from 15 different patients in the Faculty Hospital in Brno, Czech Republic. All strains were identified using biochemical tests and repetitive PCR using the (GTG)₅ primer. Doubtful identification results were confirmed by whole-cell protein analysis. The strains were assigned to the genera Lactobacillus (eight strains representing seven species), Leuconostoc (six strains representing four species) and Weissella (one strain). Antibiotic susceptibility testing was performed using the E-test and revealed high-level resistance to cotrimoxazol, metronidazole, vancomycin and teicoplanin, but nearly all strains were susceptible to erythromycin, clindamycin, ampicillin and penicillin.

References

Aguirre M & Collins MD (1993) Lactic acid bacteria and human clinical infection. J Appl Bacteriol 75: 95–107.
10.1111/j.1365-2672.1993.tb02753.x
CAS PubMed Web of Science® Google Scholar
Arpi M, Vancanneyt M, Swings J & Leisner JJ (2003) Six cases of Lactobacillus bacteraemia: identification of organisms and antibiotic susceptibility and therapy. Scand J Infect Dis 35: 404–408.
PubMed Web of Science® Google Scholar
Clinical and Laboratory Standard Institute (2006) Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; Approved Guideline M45-A. NCCLS, Wayne, PA, USA.
Google Scholar
Felten A, Barreau C, Bizet C, Lagrange PH & Philippon A (1999) Lactobacillus species identification, H₂O₂ production, and antibiotic resistance and correlation with human clinical status. J Clin Microbiol 37: 729–733.
10.1128/JCM.37.3.729-733.1999
CAS PubMed Web of Science® Google Scholar
Flaherty JD, Levett PN, Dewhirst FE, Troe TE, Warren JR & Johnson S (2003) Fatal case of endocarditis due to Weissella confusa. J Clin Microbiol 41: 2237–2239.
10.1128/JCM.41.5.2237-2239.2003
PubMed Web of Science® Google Scholar
Gevers D, Huys G & Swings J (2001) Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205: 31–36.
10.1111/j.1574-6968.2001.tb10921.x
CAS PubMed Web of Science® Google Scholar
Golan Y, Poutsiaka DD, Tozzi S, Hadley S & Snydman DR (2001) Daptomycin for line-related Leuconostoc bacteraemia. J Antimicrob Chemother 47: 364–365.
10.1093/jac/47.3.364
CAS PubMed Web of Science® Google Scholar
Green M, Wadowsky RM & Barbadora K (1990) Recovery of vancomycin-resistant Gram-positive cocci from children. J Clin Microbiol 28: 484–488.
PubMed Web of Science® Google Scholar
Husni RN, Gordon SM, Washington JA & Longworth DL (1997) Lactobacillus bacteremia and endocarditis: review of 45 cases. Clin Infect Dis 25: 1048–1055.
CAS PubMed Web of Science® Google Scholar
Isenberg HD, Vellozzi EM, Shapiro J & Rubin LG (1988) Clinical laboratory challenges in the recognition of Leuconostoc spp. J Clin Microbiol 26: 479–483.
10.1128/jcm.26.3.479-483.1988
CAS PubMed Web of Science® Google Scholar
Jimenez-Mejias ME, Becerril B, Gomez-Cia T, Del Nozal M & Palomino-Nicas J (1997) Bacteremia caused by Leuconostoc cremoris in a patient with severe burn injuries. Eur J Clin Microbiol Infect Dis 16: 533–535.
10.1007/BF01708238
CAS PubMed Web of Science® Google Scholar
Mathur S & Singh R (2005) Antibiotic resistance in food lactic acid bacteria – a review. Int J Food Microbiol 105: 281–295.
10.1016/j.ijfoodmicro.2005.03.008
CAS PubMed Web of Science® Google Scholar
Olano A, Chua J, Schroeder S, Minari A, La Salvia M & Hall G (2001) Weissella confusa (Basonym: Lactobacillus confusus) bacteremia: a case report. J Clin Microbiol 39: 1604–1607.
CAS PubMed Web of Science® Google Scholar
Pot B, Vandamme P & Kersters K (1994) Analysis of electrophoretic whole-organism protein fingerprints. Chemical Methods in Prokaryotic Systematics ( M Goodfellow & AG O'Donnell, eds), pp. 493–521. John Wiley Sons Ltd, Chichester, UK.
Google Scholar
Salminen MK, Rautelin H, Tynkkynen S, Poussa T, Saxelin M, Valtonen V & Jarvinen A (2004) Lactobacillus bacteremia, clinical significance, and patient outcome, with special focus on probiotic L. rhamnosus GG. Clin Infect Dis 38: 62–69.
10.1086/380455
PubMed Web of Science® Google Scholar
Templin KS, Crook T, Riley T, Whitener C & Aber RC (2001) Spontaneous bacterial peritonitis and bacteraemia due to Leuconostoc species in a patient with end-stage liver disease: a case report. J Infect 43: 155–157.
CAS PubMed Web of Science® Google Scholar
West PW, Al-Savan R, Foster HA, Electricwala Q, Alex A & Panigrahi D (1998) Speciation of presumptive viridans streptococci from early onset neonatal sepsis. J Med Microbiol 47: 923–928.
10.1099/00222615-47-10-923
CAS PubMed Web of Science® Google Scholar

Citing Literature

Volume49, Issue2

March 2007

Pages 192-196

Identification of lactic acid bacteria isolated from human blood cultures

Abstract

References

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

Identification of lactic acid bacteria isolated from human blood cultures

Abstract

References

Citing Literature

References

Related

Information