Molecular genetic and chemotaxonomic characterization of the terrestrial cyanobacterium Nostoc commune and its neighboring species
Hiromi Arima
Division of Biological Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan
Search for more papers by this authorNoriomi Horiguchi
Division of Biological Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan
Search for more papers by this authorShinichi Takaichi
Department of Biology, Nippon Medical School, Kawasaki, Japan
Search for more papers by this authorRumiko Kofuji
Division of Biological Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan
Search for more papers by this authorKen-Ichiro Ishida
Division of Biological Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan
Search for more papers by this authorKeishiro Wada
Division of Biological Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan
Search for more papers by this authorCorresponding Author
Toshio Sakamoto
Division of Biological Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan
Correspondence: Toshio Sakamoto, School of Natural System, College of Science and Engineering, Kanazawa University, Kakuma, Kanazawa 920-1192, Japan. Tel.: +81 76 264 6227; fax: +81 76 264 6215; e-mail: [email protected]Search for more papers by this authorHiromi Arima
Division of Biological Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan
Search for more papers by this authorNoriomi Horiguchi
Division of Biological Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan
Search for more papers by this authorShinichi Takaichi
Department of Biology, Nippon Medical School, Kawasaki, Japan
Search for more papers by this authorRumiko Kofuji
Division of Biological Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan
Search for more papers by this authorKen-Ichiro Ishida
Division of Biological Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan
Search for more papers by this authorKeishiro Wada
Division of Biological Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan
Search for more papers by this authorCorresponding Author
Toshio Sakamoto
Division of Biological Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan
Correspondence: Toshio Sakamoto, School of Natural System, College of Science and Engineering, Kanazawa University, Kakuma, Kanazawa 920-1192, Japan. Tel.: +81 76 264 6227; fax: +81 76 264 6215; e-mail: [email protected]Search for more papers by this authorAbstract
The phylogeny of the terrestrial cyanobacterium Nostoc commune and its neighboring Nostoc species was studied using molecular genetic and chemotaxonomic approaches. At least eight genotypes of N. commune were characterized by the differences among 16S rRNA gene sequences and the petH gene encoding ferredoxin-NADP+ oxidoreductase and by random amplified polymorphic DNA analysis. The genotypes of N. commune were distributed in Japan without regional specificity. The nrtP gene encoding NrtP-type nitrate/nitrite permease was widely distributed in the genus Nostoc, suggesting that the occurrence of the nrtP gene can be one of the characteristic features that separate cyanobacteria into two groups. The wspA gene encoding a 36-kDa water stress protein was only found in N. commune and Nostoc verrucosum, suggesting that these Nostoc species that form massive colonies with extracellular polysaccharides can be exclusively characterized by the occurrence of the wspA gene. Fifteen species of Nostoc and Anabaena were investigated by comparing their carotenoid composition. Three groups with distinct patterns of carotenoids were related to the phylogenic tree constructed on the basis of 16S rRNA sequences. Nostoc commune and Nostoc punctiforme were clustered in one monophyletic group and characterized by the occurrence of nostoxanthin, canthaxanthin, and myxol glycosides.
Supporting Information
| Filename | Description |
|---|---|
| fem1195-sup-0001-FigureS1.pptapplication/mspowerpoint, 1.4 MB | Fig. S1. Sampling locality of field-isolated, naturally grown colonies of Nostoc commune in Japan. |
| fem1195-sup-0002-FigureS2.pptapplication/mspowerpoint, 1.4 MB | Fig. S2. Cluster analysis using RAPD patters in the 8 genotypes of N. commune. |
| fem1195-sup-0003-TableS1.docWord document, 43.5 KB | Table S1. PCR primers. |
| fem1195-sup-0004-TableS2.docWord document, 103 KB | Table S2. Accession numbers for the nucleotide sequences of 16S rRNA gene, petH, groESL, nrtP, and wspA. |
| fem1195-sup-0005-TableS3.docWord document, 51 KB | Table S3. Accession numbers of 16S rRNA gene sequences retrieved from the EMBL/GenBank/DDBJ database. |
| fem1195-sup-0006-TableS4.docWord document, 50.5 KB | Table S4. PCR–RFLP analysis. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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