CASE REPORT
CRIMINALISTICS

A Search for Obligatory Paternal Alleles in a DNA Database to Find an Alleged Rapist in a Fatherless Paternity Case

Mark Barash M.Sc.

Forensic DNA and Biology Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police National Headquarters, Jerusalem, Israel.

Present address: Faculty of Health, Science and Medicine, Bond University, Gold Coast, QLD 4229, Australia.

Search for more papers by this author

Ayeleth Reshef M.Sc.,

Ayeleth Reshef M.Sc.

Forensic DNA and Biology Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police National Headquarters, Jerusalem, Israel.

Search for more papers by this author

Lev Voskoboinik M.Sc.,

Lev Voskoboinik M.Sc.

Forensic DNA and Biology Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police National Headquarters, Jerusalem, Israel.

Search for more papers by this author

Ashira Zamir M.Sc.,

Ashira Zamir M.Sc.

DNA Database Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police National Headquarters, Jerusalem, Israel.

Search for more papers by this author

Uzi Motro Ph.D.,

Uzi Motro Ph.D.

Department of Statistics and Department of Ecology, Evolution and Behavior, The Hebrew University of Jerusalem, Jerusalem, Israel.

Search for more papers by this author

Ron Gafny Ph.D.,

Ron Gafny Ph.D.

Forensic DNA and Biology Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police National Headquarters, Jerusalem, Israel.

Search for more papers by this author

Mark Barash M.Sc.,

Mark Barash M.Sc.

Forensic DNA and Biology Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police National Headquarters, Jerusalem, Israel.

Present address: Faculty of Health, Science and Medicine, Bond University, Gold Coast, QLD 4229, Australia.

Search for more papers by this author

Ayeleth Reshef M.Sc.,

Ayeleth Reshef M.Sc.

Forensic DNA and Biology Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police National Headquarters, Jerusalem, Israel.

Search for more papers by this author

Lev Voskoboinik M.Sc.,

Lev Voskoboinik M.Sc.

Forensic DNA and Biology Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police National Headquarters, Jerusalem, Israel.

Search for more papers by this author

Ashira Zamir M.Sc.,

Ashira Zamir M.Sc.

DNA Database Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police National Headquarters, Jerusalem, Israel.

Search for more papers by this author

Uzi Motro Ph.D.,

Uzi Motro Ph.D.

Department of Statistics and Department of Ecology, Evolution and Behavior, The Hebrew University of Jerusalem, Jerusalem, Israel.

Search for more papers by this author

Ron Gafny Ph.D.,

Ron Gafny Ph.D.

Forensic DNA and Biology Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police National Headquarters, Jerusalem, Israel.

Search for more papers by this author

First published: 05 March 2012

https://doi.org/10.1111/j.1556-4029.2012.02095.x

Citations: 4

Additional information and reprint requests:
Mark Barash, M.Sc.
Faculty of Health, Science and Medicine
Bond University
Gold Coast
QLD 4229
Australia
E-mail: [email protected]

Share a link

Email
Wechat
Bluesky

Abstract

Abstract: A sexual assault case resulted in a pregnancy, which was subsequently aborted. The alleged father of the fetus was unknown. Maternal and fetal types were obtained using the 11-locus AmpFℓSTR^® SGM Plus^® kit. The national DNA database was searched for the paternal obligatory alleles and detected two suspects who could not be excluded as father of the male fetus. Additional typing using the AmpFℓSTR^® Minifiler^™ kit, containing three additional autosomal loci, was not sufficient to exclude either suspect. Subsequent typing using the PowerPlex^® 16, containing four additional loci, and Y-Filer^™ kits resulted in excluding one suspect. Searching a database for paternal obligatory alleles can be fruitful, but is fraught with possible false positive results so that finding a match must be taken as only preliminary evidence.

References

1 Walsh PS, Metzger DA, Higuchi R. Chelex^® 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 1991; 10: 506–13.
CAS PubMed Web of Science® Google Scholar
2 Quantifiler^® kit user’s manual. The Perkin-Elmer Corporation. Foster City, CA: Applied Biosystems, 2003.
Google Scholar
3 AmpFℓSTR^® SGM Plus^® PCR Amplification Kit, user’s manual. Foster City, CA: Applied Biosystems, 2006 , http://www3.appliedbiosystems.com/cms/groups/applied_markets_support/documents/generaldocuments/cms_041049.pdf (accessed December 21, 2009).
Google Scholar
4 AmpFℓSTR^® MiniFiler^™ PCR Amplification Kit, user guide. Foster City, CA: Applied Biosystems, 2007 , http://www3.appliedbiosystems.com/cms/groups/applied_markets_support/documents/generaldocuments/cms_042748.pdf (accessed December 21, 2009).
Google Scholar
5 AmpFℓSTR^® YFiler^® Amplification Kit, user’s manual. Foster City, CA: Applied BioSystems, 2006 , http://www3.appliedbiosystems.com/cms/groups/applied_markets_support/documents/generaldocuments/cms_041477.pdf (accessed December 21, 2009).
Google Scholar
6 PowerPlex^® 16 System, technical manual. Madison, WI: Promega Corporation, 2008 , http://www.promega.com/tbs/tmd012/tmd012.pdf (accessed December 21, 2009)
Google Scholar
7 YFiler Haplotype Database, http://www6.appliedbiosystems.com/yfilerdatabase/(accessed December 21, 2009).
Google Scholar
8 Balding DJ, Nichols RA. DNA profile match probability calculation: how to allow for population stratification, relatedness, database selection and single bands. Forensic Sci Int 1994; 64: 125–40.
10.1016/0379-0738(94)90222-4
CAS PubMed Web of Science® Google Scholar
9 Budowle B, Ge J, Chakraborty R. Basic principles for estimating the rarity of Y-STR haplotypes derived from forensic evidence. Proceedings of the Eighteenth International Symposium on Human Identification; 2007 Oct 1–4; Hollywood, CA. Madison, WI: Promega Corporation, 2007.
Google Scholar
10 Boutrand L, Egyaed B, Füredi S, Mommers N, Mertens G, Vandenberghe A. Variations in primer sequences are the origin of allele drop-out at loci D13S317 and CD4. Int J Legal Med 2001; 114: 295–7.
10.1007/s004140000183
CAS PubMed Web of Science® Google Scholar
11 Hill CR, Kline MC, Mulero JJ, Lagacé RE, Chang CW, Hennessy LK, et al. Concordance study between the AmpFℓSTR MiniFiler PCR amplification kit and conventional STR typing kits. J Forensic Sci 2007; 52(4): 870–3.
10.1111/j.1556-4029.2007.00491.x
CAS PubMed Web of Science® Google Scholar
12 Brenner C. Arizona DNA database matches, 2007 , http://dna-view.com/ArizonaMatch.htm (accessed December 21, 2009).
Google Scholar
13 Devlin K. Scientific heat about cold hits, 2007 , http://www.stanford.edu/~kdevlin/Cold_Hit_Probabilities.pdf (accessed December 21, 2009).
Google Scholar
14 Budowle B, Planz JV, Chakraborty R, Callaghan TF, Eisenberg AJ. Clarification of statistical issues related to the operation of CODIS. Proceedings of the Seventeenth International Symposium on Human Identification; 2006 Oct 10–12; Nashville, TN. Madison, WI: Promega Corporation, 2006.
Google Scholar
15 Budowle B, Baechtel FS, Chakraborty R. Partial matches in heterogeneous offender databases do not call into question the validity of random match probability calculation. Int J Legal Med 2009; 123: 59–63.
10.1007/s00414-008-0239-1
PubMed Web of Science® Google Scholar
16 Song YS, Patil A, Murphy EE, Slatkin M. Average probability that a “cold hit” in a DNA database search results in an erroneous attribution. J Forensic Sci 2009; 54(1): 22–7.
10.1111/j.1556-4029.2008.00917.x
CAS PubMed Web of Science® Google Scholar
17 Bieber FR, Brenner CH, Lazer D. Finding criminals through DNA of their relatives. Science 2006; 312: 1315–6.
10.1126/science.1122655
CAS PubMed Web of Science® Google Scholar
18 Wenk RE, Chiafari FA, Gorlin J, Polesky HF. Better tolls are needed for parentage and kinship studies. Transfusion 2003; 43: 979–81.
10.1046/j.1537-2995.2003.00439.x
PubMed Web of Science® Google Scholar

Citing Literature

Volume57, Issue4

July 2012

Pages 1098-1101

A Search for Obligatory Paternal Alleles in a DNA Database to Find an Alleged Rapist in a Fatherless Paternity Case

Abstract

References

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

A Search for Obligatory Paternal Alleles in a DNA Database to Find an Alleged Rapist in a Fatherless Paternity Case

Abstract

References

Citing Literature

References

Related

Information