Volume 51, Issue 2 pp. 319-327

Changes of cytokine levels during granulocyte–colony-stimulating factor stem cell mobilization in healthy donors: association with mobilization efficiency and potential predictive significance

Daniel Lysák

Daniel Lysák

From the Department of Hematology and Oncology, University Hospital Pilsen, Czech Republic, Czech National Marrow Donor Registry (CS2); and the Laboratory of Immunoanalysis, Department of Nuclear Medicine, University Hospital Pilsen, Pilsen, Czech Republic.

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Marcela Hrabětová

Marcela Hrabětová

From the Department of Hematology and Oncology, University Hospital Pilsen, Czech Republic, Czech National Marrow Donor Registry (CS2); and the Laboratory of Immunoanalysis, Department of Nuclear Medicine, University Hospital Pilsen, Pilsen, Czech Republic.

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Jindra Vrzalová

Jindra Vrzalová

From the Department of Hematology and Oncology, University Hospital Pilsen, Czech Republic, Czech National Marrow Donor Registry (CS2); and the Laboratory of Immunoanalysis, Department of Nuclear Medicine, University Hospital Pilsen, Pilsen, Czech Republic.

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Vladimír Koza

Vladimír Koza

From the Department of Hematology and Oncology, University Hospital Pilsen, Czech Republic, Czech National Marrow Donor Registry (CS2); and the Laboratory of Immunoanalysis, Department of Nuclear Medicine, University Hospital Pilsen, Pilsen, Czech Republic.

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Jana Navrátilová

Jana Navrátilová

From the Department of Hematology and Oncology, University Hospital Pilsen, Czech Republic, Czech National Marrow Donor Registry (CS2); and the Laboratory of Immunoanalysis, Department of Nuclear Medicine, University Hospital Pilsen, Pilsen, Czech Republic.

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Tomáš Svoboda

Tomáš Svoboda

From the Department of Hematology and Oncology, University Hospital Pilsen, Czech Republic, Czech National Marrow Donor Registry (CS2); and the Laboratory of Immunoanalysis, Department of Nuclear Medicine, University Hospital Pilsen, Pilsen, Czech Republic.

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Alexandra Jungová

Alexandra Jungová

From the Department of Hematology and Oncology, University Hospital Pilsen, Czech Republic, Czech National Marrow Donor Registry (CS2); and the Laboratory of Immunoanalysis, Department of Nuclear Medicine, University Hospital Pilsen, Pilsen, Czech Republic.

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Ondřej Topolčan

Ondřej Topolčan

From the Department of Hematology and Oncology, University Hospital Pilsen, Czech Republic, Czech National Marrow Donor Registry (CS2); and the Laboratory of Immunoanalysis, Department of Nuclear Medicine, University Hospital Pilsen, Pilsen, Czech Republic.

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First published: 30 August 2010
Citations: 20
Daniel Lysák, MD, PhD, Department of Hematology and Oncology, University Hospital Pilsen, Alej Svobody 80, Pilsen 304 60; e-mail: [email protected].

Abstract

BACKGROUND: Peripheral blood stem cells are an important source of hematopoietic stem cells (HSCs) for allogeneic transplantations. Some allogeneic donors mobilize HSCs poorly in response to the granulocyte—colony-stimulating factor (G-CSF). The estimation of the mobilization result in an individual donor is difficult due to the absence of suitable predictive factors.

STUDY DESIGN AND METHODS: We analyzed the concentrations and kinetics of certain cytokines induced by G-CSF in 76 healthy donors and compared them with the mobilization efficiency.

RESULTS: The levels of the most cytokines increased after the G-CSF application: sICAM, sVCAM, MMP-9, interleukin (IL)-6, TNF-α, sE-selectin, and fibronectin. The concentrations of SDF-1α and IL-8 decreased and VEGF and fractalkine remained unchanged. The premobilization concentrations of IL-6 (p = 0.0093) and TNF-a (p = 0.0006) correlated with preapheresis CD34+ cell count. The comparison of premobilization cytokine levels between better and worse mobilizers showed a difference for TNF-α (p = 0.0006) and IL-6 (p = 0.0682). The TNF-α level below cutoff of 3.6 pg/mL implied approximately 20 times higher risk of poor mobilization (odds ratio, 19.9; p = 0.0002). The immunophenotyping of CD34+ cells suggested a negative correlation between Day +5 CD34+ count and expression of CD11a (p = 0.0319) and a positive correlation with CD44 antigen expression (p = 0.0096).

CONCLUSION: The concentrations of certain cytokines corresponded to the quality of HSC mobilization in healthy donors. Their levels measured before mobilization could probably serve as predictive factors for mobilization efficacy and prospectively detect donors who might profit from new mobilization molecules.

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