Alcoholism: Clinical and Experimental Research

Stimulatory Effect of N-Methyltyramine, a Congener of Beer, on Pancreatic Secretion in Conscious Rats

Eri Tsutsumi

From the Research Center (ET, YS), Suntory Limited, Osaka, Japan; Department of Clinical Physiology (SK, MO, KM), Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.

Search for more papers by this author

Setsuko Kanai,

Setsuko Kanai

From the Research Center (ET, YS), Suntory Limited, Osaka, Japan; Department of Clinical Physiology (SK, MO, KM), Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.

Search for more papers by this author

Minoru Ohta,

Minoru Ohta

From the Research Center (ET, YS), Suntory Limited, Osaka, Japan; Department of Clinical Physiology (SK, MO, KM), Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.

Search for more papers by this author

Yoshihide Suwa,

Yoshihide Suwa

From the Research Center (ET, YS), Suntory Limited, Osaka, Japan; Department of Clinical Physiology (SK, MO, KM), Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.

Search for more papers by this author

Kyoko Miyasaka,

Kyoko Miyasaka

From the Research Center (ET, YS), Suntory Limited, Osaka, Japan; Department of Clinical Physiology (SK, MO, KM), Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.

Search for more papers by this author

Eri Tsutsumi,

Eri Tsutsumi

From the Research Center (ET, YS), Suntory Limited, Osaka, Japan; Department of Clinical Physiology (SK, MO, KM), Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.

Search for more papers by this author

Setsuko Kanai,

Setsuko Kanai

From the Research Center (ET, YS), Suntory Limited, Osaka, Japan; Department of Clinical Physiology (SK, MO, KM), Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.

Search for more papers by this author

Minoru Ohta,

Minoru Ohta

From the Research Center (ET, YS), Suntory Limited, Osaka, Japan; Department of Clinical Physiology (SK, MO, KM), Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.

Search for more papers by this author

Yoshihide Suwa,

Yoshihide Suwa

From the Research Center (ET, YS), Suntory Limited, Osaka, Japan; Department of Clinical Physiology (SK, MO, KM), Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.

Search for more papers by this author

Kyoko Miyasaka,

Kyoko Miyasaka

From the Research Center (ET, YS), Suntory Limited, Osaka, Japan; Department of Clinical Physiology (SK, MO, KM), Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.

Search for more papers by this author

First published: 13 January 2010

https://doi.org/10.1111/j.1530-0277.2009.00893.x

Citations: 7

Reprint requests: Eri Tsutsumi, Research Center, Suntory Limited, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503 Japan; Fax: 75-962-4920; E-mail: [email protected]

Share a link

Email
Wechat
Bluesky

Abstract

Background: Alcoholic beverages stimulate gastric acid secretion and increase the appetite. Although ingested ethanol stimulates pancreatic secretion, alcoholic beverages contain several congeners. N-methyltyramine (NMT) was isolated from beer as a factor in stimulating gastric acid secretion. In this study, we examined NMT to determine whether the congener stimulated pancreatic secretion in conscious rats.

Methods: Cannulae were inserted into male Wistar rats to separately drain bile and pancreatic secretions: 2 duodenal cannulae, a gastric cannula, and an external jugular vein cannula. The rats were placed in modified Bollman-type restraint cages. After a 4-day recovery period, experiments were conducted on unanesthetized rats. Different concentrations of NMT (5, 25, and 50 μg/kg) solutions were infused into the stomach. To examine the mechanism, the effects of the proton pump inhibitor, cholecystokinin (CCK-BR) antagonist (YM022), CCK-AR antagonist (CR1505), and atropine were administered prior to the NMT (25 μg/kg) infusion. The effect of intravenous infusion of NMT (7.5 μg/kg) was then determined. Moreover, dispersed acini were prepared, and the effect of different concentrations of NMT on amylase release was determined.

Results: Intragastric administration of NMT significantly increased pancreatic exocrine secretion in a dose-dependent manner. Atropine eliminated the stimulatory effect of NMT, but the infusion of the proton pump inhibitor, YM022, and CR1505 did not. Intravenous infusion of NMT did not affect pancreatic secretion, and NMT did not stimulate amylase release in vitro.

Conclusions: N-methyltyramine stimulates pancreatic secretion via the cholinergic gastro-pancreatic reflex. The NMT content in beer was 2 mg/l, so that if a person weighing 60 kg consumes a 750 ml of beer, 25 μg/kg NMT will be ingested. Therefore, the stimulatory effect of beer on pancreatic secretion was produced not only by ethanol but also by the congener, NMT.

References

Amsterdam A, Jamieson JD (1972) Structural and functional characterization of isolated pancreatic exocrine cells. Proc Natl Acad Sci USA 69: 3028–3032.
10.1073/pnas.69.10.3028
CAS PubMed Web of Science® Google Scholar
Chari ST, Harder H, Teyssen S, Knodel C, Riepl RL, Singer MV (1996) Effect of beer, yeast-fermented glucose, and ethanol on pancreatic enzyme secretion in healthy human subjects. Dig Dis Sci 41: 1216–1224.
10.1007/BF02088240
CAS PubMed Web of Science® Google Scholar
Demol P, Singer MV, Hotz J, Hoffmann U, Hanssen LE, Eysselein VE, Goebell H (1986) Action of intragastric ethanol on pancreatic exocrine secretion in relation to the interdigestive gastrointestinal motility in humans. Arch Int Physiol Biochim 94: 251–259.
10.3109/13813458609071425
CAS PubMed Web of Science® Google Scholar
Ishizaki K, Kinbara S, Kawamura M, Kimura K, Shiratori K, Takeuchi T (2003) Effect of cholecystokinin 1 receptor antagonist loxiglumide (CR1505) on basal pancreatic exocrine secretion in conscious rats. Pancreas 26: 87–91.
10.1097/00006676-200301000-00015
CAS PubMed Web of Science® Google Scholar
Izquierdo-Pulido M, Mariné-Font A, Vidal-Carou MC (1994) Biogenic amines formation during malting and brewing. J Food Sci 59: 1104–1107.
10.1111/j.1365-2621.1994.tb08201.x
CAS Web of Science® Google Scholar
Kirkwood S, Marion L (1950) The biogenesis of alkaloids. I. The isolation of N- methyltyramine from barley. J Am Chem Soc 72: 2522–2523.
10.1021/ja01162a050
CAS Web of Science® Google Scholar
Kölbel CB, Singer MV, Dorsch W, Krege P, Eysselein VE, Layer P, Goebell H (1988) Pancreatic and gastric responses to gastric versus jejunal beer in humans. Pancreas 3: 89–94.
10.1097/00006676-198802000-00015
CAS PubMed Web of Science® Google Scholar
Larsson H, Carlsson E, Junggren U, Olbe L, Sjostrand SE, Skanberg I, Sundell G (1983) Inhibition of gastric acid secretion by omeprazole in the dog and rat. Gastroenterology 85: 900–907.
10.1016/0016-5085(83)90442-0
CAS PubMed Web of Science® Google Scholar
Llanos OL, Swierczek JS, Teichmann RK, Rayford PL, Thompson JC (1977) Effect of alcohol on the release of secretin and pancreatic secretion. Surgery 81: 661–667.
CAS PubMed Web of Science® Google Scholar
Masuda M, Miyasaka K, Funakoshi A (1997) Involvement of 5-hydroxytryptamine (5-HT)3 receptor mechanisms in regulation of basal pancreatic secretion in conscious rats. J Auton Nerv Syst 62: 58–62.
10.1016/S0165-1838(96)00109-9
CAS PubMed Web of Science® Google Scholar
Miyasaka K, Green GM (1983) Effect of atropine on rat basal pancreatic secretion during return or diversion of bile-pancreatic juice. Proc Soc Exp Biol Med 174: 187–192.
10.3181/00379727-174-41723
CAS PubMed Web of Science® Google Scholar
Miyasaka K, Kanai K, Ohta M, Funakoshi A (2005) Stimulatory effect of ethanol on pancreatic secretion in conscious rats requires CCK-A receptor. Pancreas 30: e22–e28.
PubMed Web of Science® Google Scholar
Miyasaka K, Sazaki N, Funakoshi A, Matsumoto M, Kitani K (1993) Two mechanisms of inhibition by bile on luminal feedback regulation of rat pancreas. Gastroenterology 104: 1780–1785.
CAS PubMed Web of Science® Google Scholar
Nagamatsu T, Sato K, Arao T (2001) Low intensity exercise increased plasma gastrin and gastric acid secretion. Biomedical Research 22: 1–6.
10.2220/biomedres.22.1
CAS Web of Science® Google Scholar
Niebergall-Rorh E, Singer MV (2006) Enteropancreatic reflexes mediating the pancreatic enzyme response to nutrients. Auton Neurosci 125: 62–69.
10.1016/j.autneu.2006.01.003
CAS PubMed Web of Science® Google Scholar
Singer MV (1993) Neurohormonal control of pancreatic enzyme secretion in animals, in The Pancreas: Biology, Pathobiology, and Disease, 2nd ed. ( VLW Go, EP Dimagno, JD Gardner, E Lebental, HA Reber, GA Sheele eds), pp 425–448. Raven Press, New York.
Google Scholar
Takinami Y, Yuki H, Nishida A, Akuzawa S, Uchida A, Takemoto Y, Ohta M, Satoh M, Semple G, Miyata K (1997) YF476 is a new potent and selective gastrin/cholecystokinin-B receptor antagonist in vitro and in vivo. Aliment Pharmacol Ther 11: 113–120.
10.1046/j.1365-2036.1997.110281000.x
CAS PubMed Web of Science® Google Scholar
Tiscornia OM, Gullo L, Sarles H, Mott CB, Brasca A, Devaux MA, Palasciano G, Hage G (1974) Effects of intragastric and intraduodenal ethanol on canine exocrine pancreatic secretion. Digestion 10: 52–60.
10.1159/000197522
CAS PubMed Web of Science® Google Scholar
Wheatley IC, Barbezat GO, Hickman R, Terblanche J (1975) The effect of acute ethanol administration on the exocrine pancreatic secretion of the pig. Br J Surg 62: 707–712.
10.1002/bjs.1800620909
CAS PubMed Web of Science® Google Scholar
Yokoo Y, Kohda H, Kusumoto A, Naoki H, Matsumoto N, Amachi T, Suwa Y, Fukazawa H, Ishida H, Tsuji K, Nukaya H (1999) Isolation from beer and structural determination of a potent stimulant of gastrin release. Alcohol Alcohol 34: 161–168.
10.1093/alcalc/34.2.161
CAS PubMed Web of Science® Google Scholar

Citing Literature

Volume34, Issues1

Special Issue:Proceedings of the 26th Meeting of the Japanese Society for Biomedical Research on Alcohol, Sapporo, Japan, March 9‐10, 2006

February 2010

Pages S14-S17

Stimulatory Effect of N-Methyltyramine, a Congener of Beer, on Pancreatic Secretion in Conscious Rats

Abstract

References

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

Stimulatory Effect of N-Methyltyramine, a Congener of Beer, on Pancreatic Secretion in Conscious Rats

Abstract

References

Citing Literature

References

Related

Information