Dr. Woon Yi Baek, Department of Anesthesiology, School of Medicine, Kyungpook National University, 50 Samduck 2ga, Jung gu, Daegu 700-721, South Korea. E-mail: [email protected]

Read the full text

About

PDF

Tools

Share a link

Email
Wechat
Bluesky

Abstract

Bovine chromaffin cells (BCCs) are well known to have analgesic effect to reduce acute or chronic pain when transplanted in the subarachnoid space and have been considered as an alternative therapy for pain management. However, due to recent concerns over risks associated with prion transmission, porcine tissue is considered to be an alternate xenogeneic source for clinical use. In the present study, we investigated whether microencapsulated porcine adrenal medullary chromaffin cells (PCCs) also have analgesic effect to reduce allodynia caused by neuropathic pain in chronic constriction injury model of rat. PCCs were isolated from a porcine adrenal medulla and then microencapsulated with alginate and poly. In in vitro tests, the microencapsulated PCCs were investigated whether they have an ability to release catecholamines responding to nicotine stimulation. The levels of catecholamines released from the microencapsulated PCCs were significantly higher than from microencapsulated BCCs. In addition, the microencapsulated PCCs released catecholamines and met-enkephalin responding to cerebral spinal fluid (CSF) retrieved from a neuropathic pain model. In in vivo tests, implantation of microencapsulated PCCs reduced both mechanical and cold allodynia in chronic constriction injury model of a rat whereas the microencapsulated BCCs reduced only cold allodynia under the same conditions. The injection of antagonist of opioid peptides reversed the reduction of cold allodynia in microencapsulated PCC-received animal. The levels of catecholamines in the CSF of rats after implantation of microencapsulated PCCs were significantly higher than in the control group. These data suggest that microencapsulated PCCs may be another effective source for the treatment of neuropathic pain.

REFERENCES

1 Kim YM, Jeon YH, Jin GC, Lim JO, Baek WY. Immunoisolated chromaffin cells implanted into the subarachnoid space of rats reduce cold alloynia in a model of neuropathic pain: a novel application of microencapsulation technology. Artif Organs 2004; 28: 1059–66.
10.1111/j.1525-1594.2004.00024.x
PubMed Web of Science® Google Scholar
2 Hentall ID, Sagen JC. The alleviation of pain by cell transplantation. Brain Res 2000; 127: 535–49.
10.1016/S0079-6123(00)27027-1
PubMed Google Scholar
3 Descosterd I, Bucher E, Gilliard N, Saydoff J, Suru AD, Aebischer P. Intrathecal implants of bovine chromaffin cells alleviate mechanical alloydnia in a rat model of neuropathic pain. Pain 1998; 76: 159–66.
10.1016/S0304-3959(98)00044-X
PubMed Web of Science® Google Scholar
4 Sagen J, Pappas GD, Pollard HB. Analgesia induced by isolated bovine chromaffin cells implanted in rat spinal cord. Proc Natl Acad Sci USA 1986; 83: 7522–6.
10.1073/pnas.83.19.7522
CAS PubMed Web of Science® Google Scholar
5 Joseph JM, Goddard MB, Mills J. Transplantation of encapsulated bovine chromaffin cells in the sheep subarachnoid space: a preclinical study for the treatment of cancer pain. Cell Transplant 1994; 3: 355–64.
10.1177/096368979400300502
CAS PubMed Web of Science® Google Scholar
6 Pappas GD, Lazorthes Y, Bes JC, Tafani M, Winni AP. Relief of intractable cancer pain by human chromaffin cell transplants: experience at two medical centers. Neurol Res 1997; 19: 71–7.
10.1080/01616412.1997.11740775
CAS PubMed Web of Science® Google Scholar
7 Lu Y, Jing R, Yeomans DC, Pappas GD. Porcine chromaffin cells, culture, and transplant for antinociceptive effect in rodents and primates. Neurol Res 2004; 26: 707–12.
10.1179/016164104225018018
PubMed Web of Science® Google Scholar
8 Bennett GJ, Xie YK. A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man. Pain 1988; 33: 87–107.
10.1016/0304-3959(88)90209-6
CAS PubMed Web of Science® Google Scholar
9 Bruce GL. Adrenal medullary chromaffin cells in vitro. Physiol Rev 1984; 64: 1103–60.
PubMed Web of Science® Google Scholar
10 Hentall ID, Sagen J. Spinal CSF from rats with painful peripheral neuropathy evokes catecholamine release from chromaffin cells in vitro. Neurosci Lett 2000; 285: 95–8.
10.1016/S0304-3940(00)01112-5
Web of Science® Google Scholar
11 Chaplan SR, Bach FW, Pogrel JW, Chung JM, Yaksh TL. Quantitative assessment of tactile allodynia in the rat paw. J Neurosci Methods 1994; 53: 55–63.
10.1016/0165-0270(94)90144-9
CAS PubMed Web of Science® Google Scholar
12 Choi Y, Woon YW, Ha HS, Kim SH, Chung JM. Behavioral signs of ongoing pain and cold allodynia in a rat model of neuropathic pain. Pain 1994; 59: 369–76.
10.1016/0304-3959(94)90023-X
CAS PubMed Web of Science® Google Scholar
13 Sagen J, Wang H, Pappas GD. Adrenal medullary implants in the rat spinal cord reduce nociception in a chronic pain model. Pain 1990; 42: 69–70.
10.1016/0304-3959(90)91093-X
CAS PubMed Web of Science® Google Scholar
14 Buscher E, Goddard M, Heyd B, et al. Immunoisolated xenogeneic chromaffin cell therapy for chronic pain. Anesthesiology 1996; 85: 1005–12.
10.1097/00000542-199611000-00007
PubMed Web of Science® Google Scholar
15 Sol JC, Sallerin B, Larrue S, et al. Intrathecal xenogeneic chromaffin cell grafts reduce nociceptive behavior in a rodent tonic pain model. Exp Neurol 2004; 186: 198–211.
10.1016/j.expneurol.2003.10.018
PubMed Web of Science® Google Scholar
16 Lindner MD, Francis JM, Mcmermott PE, et al. Numerous adrenal chromaffin cell preparations fail to produce analgesic effects in the formalin test or in tests of acute pain even with nicotine stimulation. Pain 2000; 88: 177–88.
10.1016/S0304-3959(00)00324-9
PubMed Web of Science® Google Scholar
17 Gulwadi AG, Hoane MR, Saydoff JA, Frydel BR, Lindner MD. No detectable analgesic effects in the formalin test even with one million bovine adrenal chromaffin cells. Pain 2002; 99: 263–71.
10.1016/S0304-3959(02)00120-3
PubMed Web of Science® Google Scholar
18 Dwyer KM, Cowan PJ, D'Apice AJF. Xenotransplantation: past achievement and future promise. Heart Lung Circ 2002; 11: 32–41.
10.1046/j.1444-2892.2002.00110.x
PubMed Google Scholar
19 Cozzi E, Bosio E, Seveso M, Vadori M, Ancona E. Xenotransplantation—current status and future perspectives. Br Med Bull 2006; 75: 99–114.
Google Scholar
20 Hama AT, Sagen J. Alleviation of neuropathic pain symptoms by xenogeneic chromaffin cell grafts in the spinal subarachnoid space. Brain Res 1994; 651: 183–93.
10.1016/0006-8993(94)90696-3
CAS PubMed Web of Science® Google Scholar
21 Waymire JC, Bennett WF, Boehme R, Hankins L, Gilmer Waymire K, Haycock JW. Bovine adrenal chromaffin cells: high-yield purification and viability in suspension culture. J Neurosci Methods 1983; 7: 329–51.
10.1016/0165-0270(83)90026-2
CAS PubMed Web of Science® Google Scholar

Citing Literature

Volume33, Issue3

March 2009

Pages 240-249

Reduction of Allodynia by Intrathecal Transplantation of Microencapsulated Porcine Chromaffin Cells

Abstract

REFERENCES

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

Reduction of Allodynia by Intrathecal Transplantation of Microencapsulated Porcine Chromaffin Cells

Abstract

REFERENCES

Citing Literature

References

Related

Information