Morphological Characteristics and Proliferation of Keratocytes Cultured Under Simulated Microgravity
Corresponding Author
Jiansu Chen
Ophthalmology Department, Medical College, Jinan University, Guangzhou;
Dr. Jiansu Chen, Ophthalmology Department, Medical College, Jinan University, 601 West Huangpu Avenue, Guangzhou 510632, China. E-mail: [email protected]Search for more papers by this authorRui Chen
Ophthalmology Department, Medical College, Jinan University, Guangzhou;
Ophthalmic Center, the Second People's Hospital of Foshan, Foshan; and
Search for more papers by this authorShujing Gao
College of Animal Science, South China Agricultural University, Guangzhou, China
Search for more papers by this authorCorresponding Author
Jiansu Chen
Ophthalmology Department, Medical College, Jinan University, Guangzhou;
Dr. Jiansu Chen, Ophthalmology Department, Medical College, Jinan University, 601 West Huangpu Avenue, Guangzhou 510632, China. E-mail: [email protected]Search for more papers by this authorRui Chen
Ophthalmology Department, Medical College, Jinan University, Guangzhou;
Ophthalmic Center, the Second People's Hospital of Foshan, Foshan; and
Search for more papers by this authorShujing Gao
College of Animal Science, South China Agricultural University, Guangzhou, China
Search for more papers by this authorAbstract
Abstract: This study probed the changes of keratocytes cultured under simulated microgravity. Keratocytes were isolated from rabbit corneas using collagenase digestion method. Cells were seeded in a 55-mL capacity high-aspect-ratio vessel (HARV) of rotary cell culture system (RCCS) at a density of 1 × 104 cells/mL. Dehydrated bovine acellular corneal stroma (5 × 5 × 1 mm, n = 30) was used as a carrier for keratocyte culture. Rotational speed was set at 15, 20, and 30 rpm in the first, second, and third week of culture, respectively. Histological evaluation showed that keratocytes in simulated microgravity culture grew into carriers, but those under conventional gravity grew on the surface of carriers. Scanning electron microscopic evaluation showed that after 19 days in culture, keratocytes on the carriers were spherical and spread in the spaces among the collagen fibers. Cells were dendritic or spindle shaped, and they developed many foot processes linked with surrounding cells. The absorbance values of the simulated microgravity group were significantly higher (P < 0.01) than that of the conventional group from 10 to 19 days of culture. The RCCS obviously enhanced the proliferation of rabbit keratocytes and facilitated the cells' growth into or on the dehydrated bovine acellular corneal stroma. Cells showed more natural morphology.
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