The dynamin-related protein DRP-1 and the insulin signaling pathway cooperate to modulate Caenorhabditis elegans longevity
Christine C. Yang
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14850, USA
Search for more papers by this authorDiana Chen
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14850, USA
Search for more papers by this authorSiu Sylvia Lee
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14850, USA
Search for more papers by this authorLudivine Walter
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14850, USA
Search for more papers by this authorChristine C. Yang
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14850, USA
Search for more papers by this authorDiana Chen
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14850, USA
Search for more papers by this authorSiu Sylvia Lee
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14850, USA
Search for more papers by this authorLudivine Walter
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14850, USA
Search for more papers by this authorSummary
Here, we report that inactivation of the Caenorhabditis elegans dynamin-related protein DRP-1, a key component responsible for mitochondrial fission and conserved from yeast to humans, dramatically enhanced the effect of reduced insulin signaling (IIS) to extend lifespan. This represents the first report of a beneficial impact of manipulating mitochondrial dynamics on animal lifespan and suggests that mitochondrial morphology and IIS cooperate to modulate aging.
Supporting Information
Fig. S1 drp-1 RNAi affects mitochondrial morphology in muscle cells of the body wall. GFP expression of a Pmyo-3::GFPmt transgene in wild-type worms treated with empty vector in (A) or drp-1 RNAi in (B).
Fig. S2 The extended longevity and the sensitivity to heat stress of the age-1;drp-1 mutant compared to the age-1 mutant can be rescued by introduction of a transgene overexpressing drp-1.
Table S1 Quantitative data and statistical analyses of mean adult lifespan presented in Fig. 1.
Table S2 Effect of drp-1 RNAi on the mean lifespan of long-lived worms undergoing bacteria deprivation and long-lived mitochondrial mutant worms.
Table S3 Quantitative data and statistical analyses of survival on paraquat presented in Fig. 2.
Data S1 Materials and methods.
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