Age-dependent cardiomyopathy in mitochondrial mutator mice is attenuated by overexpression of catalase targeted to mitochondria
Dao-Fu Dai
Department of Pathology, University of Washington, Seattle, WA 98195, USA
Search for more papers by this authorTony Chen
Department of Pathology, University of Washington, Seattle, WA 98195, USA
Search for more papers by this authorJonathan Wanagat
Department of Medicine, Division of Geriatrics, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
Search for more papers by this authorMichael Laflamme
Department of Pathology, University of Washington, Seattle, WA 98195, USA
Search for more papers by this authorMary J Emond
Biostatistics, University of Washington, Seattle, WA 98195, USA
Search for more papers by this authorCalvin P. Ngo
Department of Pathology, University of Washington, Seattle, WA 98195, USA
Search for more papers by this authorTomas A. Prolla
Departments of Genetics & Medical Genetics, University of Wisconsin-Madison, WI 53706, USA
Search for more papers by this authorPeter S. Rabinovitch
Department of Pathology, University of Washington, Seattle, WA 98195, USA
Search for more papers by this authorDao-Fu Dai
Department of Pathology, University of Washington, Seattle, WA 98195, USA
Search for more papers by this authorTony Chen
Department of Pathology, University of Washington, Seattle, WA 98195, USA
Search for more papers by this authorJonathan Wanagat
Department of Medicine, Division of Geriatrics, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
Search for more papers by this authorMichael Laflamme
Department of Pathology, University of Washington, Seattle, WA 98195, USA
Search for more papers by this authorMary J Emond
Biostatistics, University of Washington, Seattle, WA 98195, USA
Search for more papers by this authorCalvin P. Ngo
Department of Pathology, University of Washington, Seattle, WA 98195, USA
Search for more papers by this authorTomas A. Prolla
Departments of Genetics & Medical Genetics, University of Wisconsin-Madison, WI 53706, USA
Search for more papers by this authorPeter S. Rabinovitch
Department of Pathology, University of Washington, Seattle, WA 98195, USA
Search for more papers by this authorSummary
Mitochondrial defects have been found in aging and several age-related diseases. Mice with a homozygous mutation in the exonuclease encoding domain of mitochondrial DNA polymerase gamma (Polgm/m) are prone to age-dependent accumulation of mitochondrial DNA mutations and have shown a broad spectrum of aging-like phenotypes. However, the mechanism of cardiac phenotypes in relation to the role of mitochondrial DNA mutations and oxidative stress in this mouse model has not been fully addressed. We demonstrate age-dependent cardiomyopathy in Polgm/m mice, which by 13–14 months of age displays marked cardiac hypertrophy and dilatation, impairment of systolic and diastolic function, and increased cardiac fibrosis. This age-dependent cardiomyopathy is associated with increases in mitochondrial DNA (mtDNA) deletions and protein oxidative damage, increased expression of apoptotic and senescence markers, as well as a decline in signaling for mitochondrial biogenesis. The relationship of these changes to mitochondrial reactive oxygen species (ROS) was tested by crossing Polgm/m mice with mice that overexpress mitochondrial targeted catalase (mCAT). All of the above phenotypes were partially rescued in Polgm/m/mCAT mice. These data indicate that accumulation of mitochondrial DNA damage with age can lead to cardiomyopathy and that this phenotype is partly mediated by mitochondrial oxidative stress.
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