Clinical and molecular microbiological characteristics of carbapenem-resistant Acinetobacter baumannii strains in an NICU

Zhangxue Hu

Department of Pediatrics

Zheng Wang

Center for Hospital Infection Control, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing

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Ding Liu,

Corresponding Author

Ding Liu

Center for Hospital Infection Control, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing

Ding Liu, MD, Center for Hospital Infection Control, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China. Email: [email protected]Search for more papers by this author

Ping Chen,

Ping Chen

Center for Hospital Infection Control, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing

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Hao Wang,

Hao Wang

Center for Hospital Infection Control, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing

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Yao Chen,

Yao Chen

Center for Hospital Infection Control, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing

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Xilong Zhao,

Xilong Zhao

Department of Pathology, Kunming General Hospital of Chengdu Military Region, Kunming, China

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Yuan Shi,

Yuan Shi

Department of Pediatrics

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Zhangxue Hu,

Zhangxue Hu

Department of Pediatrics

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Zheng Wang,

Zheng Wang

Center for Hospital Infection Control, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing

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Ding Liu,

Corresponding Author

Ding Liu

Center for Hospital Infection Control, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing

Ping Chen,

Ping Chen

Center for Hospital Infection Control, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing

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Hao Wang,

Hao Wang

Center for Hospital Infection Control, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing

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Yao Chen,

Yao Chen

Center for Hospital Infection Control, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing

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Xilong Zhao,

Xilong Zhao

Department of Pathology, Kunming General Hospital of Chengdu Military Region, Kunming, China

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Yuan Shi,

Yuan Shi

Department of Pediatrics

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First published: 23 May 2011

https://doi.org/10.1111/j.1442-200X.2011.03397.x

Citations: 6

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Abstract

Background: Seventeen cases of Acinetobacter baumannii infection in a neonatal intensive care unit (NICU) were evaluated. The strains were characterized as resistant to carbapenems. The aim of the present study was therefore to investigate the clinical and molecular epidemiological characteristics of the 17 carbapenem-resistant A. baumannii strains.

Methods: Samples were isolated from blood or sputum from the patients in the NICU, cultured using conventional techniques and an automated system. Multiplex polymerase chain reaction (PCR) was used to detect blaOXA-51-like, blaOXA-23-like, OXA-24, OXA-58 and Ambler class B carbapenemases. The genotype of the strains was identified on pulsed-field gel electrophoresis (PFGE).

Results: BlaOXA-23 was detected in all of the isolates. PFGE genotype analysis suggested three clones among the 17 strains. Two clones were isolated from other wards of the hospital including the adult ICU and Department of Pulmonology. The other clone was proved to be the first appearance in the hospital as genotype analysis.

Conclusion: BlaOXA-23 was the drug-resistant gene that made A. baumannii resistant to carbepenem. The source of blaOXA-23 in the 17 isolates was different.

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Volume53, Issue6

December 2011

Pages 867-872

Clinical and molecular microbiological characteristics of carbapenem-resistant Acinetobacter baumannii strains in an NICU

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Clinical and molecular microbiological characteristics of carbapenem-resistant Acinetobacter baumannii strains in an NICU

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