Association of 5-hydroxytryptamine (serotonin) receptor 4 (5-HTR4) gene polymorphisms with asthma
TAE-HOON KIM
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Search for more papers by this authorSUNG-HYE AN
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Search for more papers by this authorJI-YEON CHA
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Search for more papers by this authorEUN-KYONG SHIN
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Search for more papers by this authorJI-YEON LEE
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Search for more papers by this authorSANG-HYUK YOON
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Search for more papers by this authorYOUNG-MOK LEE
Division of Allergy and Respiratory Medicine, Soonchunhyang University Hospital
Search for more papers by this authorSOO-TAEK UH
Division of Allergy and Respiratory Medicine, Soonchunhyang University Hospital
Search for more papers by this authorSUNG-WOO PARK
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Search for more papers by this authorJONG-SOOK PARK
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Search for more papers by this authorYOUNG-HOON KIM
Division of Allergy and Respiratory Medicine, Soonchunhyang University Hospital, Gumi-si
Search for more papers by this authorJAE-SUNG CHOI
Division of Allergy and Respiratory Medicine, Soonchunhyang University Hospital, Cheonan, Korea
Search for more papers by this authorSOO-OK LEE
Department of Life Science, Sogang University, Seoul
Search for more papers by this authorBYUNG-LAE PARK
Department of Genetic Epidemiology, SNP Genetics, Inc.
Search for more papers by this authorHYUNG-DOO SHIN
Department of Genetic Epidemiology, SNP Genetics, Inc.
Department of Life Science, Sogang University, Seoul
Search for more papers by this authorCorresponding Author
CHOON-SIK PARK
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Choon-Sik Park, Division of Allergy and Respiratory Medicine, Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, 1174, Jung-Dong, Wonmi-Ku, Bucheon, Kyeonggi-Do 420-020, Korea. Email: [email protected]Search for more papers by this authorTAE-HOON KIM
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Search for more papers by this authorSUNG-HYE AN
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Search for more papers by this authorJI-YEON CHA
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Search for more papers by this authorEUN-KYONG SHIN
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Search for more papers by this authorJI-YEON LEE
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Search for more papers by this authorSANG-HYUK YOON
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Search for more papers by this authorYOUNG-MOK LEE
Division of Allergy and Respiratory Medicine, Soonchunhyang University Hospital
Search for more papers by this authorSOO-TAEK UH
Division of Allergy and Respiratory Medicine, Soonchunhyang University Hospital
Search for more papers by this authorSUNG-WOO PARK
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Search for more papers by this authorJONG-SOOK PARK
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Search for more papers by this authorYOUNG-HOON KIM
Division of Allergy and Respiratory Medicine, Soonchunhyang University Hospital, Gumi-si
Search for more papers by this authorJAE-SUNG CHOI
Division of Allergy and Respiratory Medicine, Soonchunhyang University Hospital, Cheonan, Korea
Search for more papers by this authorSOO-OK LEE
Department of Life Science, Sogang University, Seoul
Search for more papers by this authorBYUNG-LAE PARK
Department of Genetic Epidemiology, SNP Genetics, Inc.
Search for more papers by this authorHYUNG-DOO SHIN
Department of Genetic Epidemiology, SNP Genetics, Inc.
Department of Life Science, Sogang University, Seoul
Search for more papers by this authorCorresponding Author
CHOON-SIK PARK
Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon
Choon-Sik Park, Division of Allergy and Respiratory Medicine, Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, 1174, Jung-Dong, Wonmi-Ku, Bucheon, Kyeonggi-Do 420-020, Korea. Email: [email protected]Search for more papers by this authorABSTRACT
Background and objective: The neurotransmitter, 5-hydroxytryptamine, acts as an immunomodulator by stimulating the release of inflammatory cytokines and regulating the function of dendritic cells and monocytes. The 5-hydroxytryptamine receptor 4 (HTR4) gene is located in a region previously linked to an increased risk of asthma and atopy. The aim of this study was to investigate the association between HTR4 and asthma.
Methods: Thirty-two single nucleotide polymorphisms (SNP) in HTR4 were investigated by direct sequencing of 24 DNA samples from unrelated Korean subjects.
Results: The 32 genetic variants comprised 22 intronic SNP, two SNP in the 3′-untranslated region (exon 7) and eight SNP in the 3′-downstream region. Logistic regression analysis showed that two intronic polymorphisms were significantly associated with the risk of asthma. Two minor HTR4 alleles, +142828G > A and +122769G > A, occurred at significantly higher frequencies in the asthmatic group than in the healthy control group (49.59% vs 42.29%, P = 0.003, and 47.99% vs 40.35%, P = 0.008, respectively), and these differences remained significant after correction for multiple testing (P = 0.05, dominant mode of inheritance; and P = 0.03, dominant mode, respectively). Haplotype analysis revealed three haplotype blocks. The frequency of haplotype 1 in block 2 was significantly higher in asthmatics (P = 0.003, dominant mode), whereas the frequency of haplotype 4 in block 3 was significantly lower in asthmatics (P = 0.0009, dominant mode).
Conclusions: SNP and haplotypes of the HTR4 gene were associated with the asthma phenotype and genetic variation of HTR4 may affect susceptibility to the development of asthma.
Supporting Information
Table S1 Oligonucleotide sequences for single nucleotide polymorphisms discovery.
Table S2 Oligonucleotide sequences for single nucleotide polymorphisms genotyping.
Table S3 Comparisons of genotype and haplotype distributions between asthmatics and normal controls.
| Filename | Description |
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| RESP_1963_sm_TableS1-3.doc401 KB | Supporting info item |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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