Characterization of Numb expression in astrocytomas
Benedict Yan
Department of Pathology, National University Hospital,
Search for more papers by this authorKakoli Das
Department of Pathology, National University Hospital,
Oncology Research Institute,
Search for more papers by this authorWai Hoe Ng
Department of Neurosurgery, National Neuroscience Institute, Singapore, and
Search for more papers by this authorChinghway Lim
Department of Statistics, University of California, Berkeley, California, USA
Search for more papers by this authorKoh Shiuan
Department of Physiology, Yong Loo Lin's School of Medicine, National University of Singapore,
Search for more papers by this authorCelestial T. Yap
Department of Physiology, Yong Loo Lin's School of Medicine, National University of Singapore,
Search for more papers by this authorCorresponding Author
Manuel Salto-Tellez
Department of Pathology, National University Hospital,
Oncology Research Institute,
Manuel Salto-Tellez, LMS/MD, FRCPath, Department of Pathology, National University of Singapore/National University Hospital, 5 Lower Kent Ridge Road, Singapore 119074, Singapore. Email: [email protected]Search for more papers by this authorBenedict Yan
Department of Pathology, National University Hospital,
Search for more papers by this authorKakoli Das
Department of Pathology, National University Hospital,
Oncology Research Institute,
Search for more papers by this authorWai Hoe Ng
Department of Neurosurgery, National Neuroscience Institute, Singapore, and
Search for more papers by this authorChinghway Lim
Department of Statistics, University of California, Berkeley, California, USA
Search for more papers by this authorKoh Shiuan
Department of Physiology, Yong Loo Lin's School of Medicine, National University of Singapore,
Search for more papers by this authorCelestial T. Yap
Department of Physiology, Yong Loo Lin's School of Medicine, National University of Singapore,
Search for more papers by this authorCorresponding Author
Manuel Salto-Tellez
Department of Pathology, National University Hospital,
Oncology Research Institute,
Manuel Salto-Tellez, LMS/MD, FRCPath, Department of Pathology, National University of Singapore/National University Hospital, 5 Lower Kent Ridge Road, Singapore 119074, Singapore. Email: [email protected]Search for more papers by this authorAbstract
During early neurodevelopment, asymmetric segregation of Numb in mitotic progenitor cells influences the fate of daughter cells, whereby one daughter retains the progenitor phenotype while the other proceeds along a differentiation pathway. Numb has also been reported to function as a tumor suppressor in breast cancers and medulloblastomas. Given its role in maintaining neural progenitor pools in animal models and its reported role as a tumor suppressor, Numb could potentially contribute to astrocytoma oncogenesis. We characterized Numb expression in both human astrocytoma tissue samples and glioblastoma cell lines. We found that Numb is expressed in all grades of astrocytomas, being predominantly cytoplasmic in higher-grade astrocytomas but nuclear in pilocytic astrocytomas. Numb is also present in normal neurons, but not in normal astrocytes. In cultured glioblastoma cells, Numb concentrates in the perinuclear region and process tips. Numb expression in astrocytomas recapitulates that of progenitor cells during neurodevelopment, and suggests a role for Numb in astrocytoma oncogenesis.
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