Dynamic changes in Th1, Th17, and FoxP3+ T cells in patients with acute cellular rejection after cardiac transplantation
Sihua Wang
Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan
Sihua Wang and Jun Li contributed equally to this work.
Search for more papers by this authorJun Li
Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan
Sihua Wang and Jun Li contributed equally to this work.
Search for more papers by this authorAini Xie
Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan
Search for more papers by this authorGuohua Wang
Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan
Search for more papers by this authorNi Xia
Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan
Search for more papers by this authorPing Ye
Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan
Search for more papers by this authorLu Rui
Department of Cardiovascular Surgery, Fu Wai Hospital, Beijing, China
Search for more papers by this authorJiahong Xia
Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan
Search for more papers by this authorSihua Wang
Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan
Sihua Wang and Jun Li contributed equally to this work.
Search for more papers by this authorJun Li
Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan
Sihua Wang and Jun Li contributed equally to this work.
Search for more papers by this authorAini Xie
Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan
Search for more papers by this authorGuohua Wang
Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan
Search for more papers by this authorNi Xia
Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan
Search for more papers by this authorPing Ye
Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan
Search for more papers by this authorLu Rui
Department of Cardiovascular Surgery, Fu Wai Hospital, Beijing, China
Search for more papers by this authorJiahong Xia
Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan
Search for more papers by this authorConflict of interest: All the authors have no financial or commercial conflict of interest. This work was supported by National Natural Science Fund of China, 81070205, and 973 State Key Development Program of Basic Research of China, 2010CB535012.
Abstract
Wang S, Li J, Xie A, Wang G, Xia N, Ye P, Rui L, Xia J. Dynamic changes in Th1, Th17, and FoxP3+ T cells in patients with acute cellular rejection after cardiac transplantation. Clin Transplant 2011: 25: E177–E186. © 2010 John Wiley & Sons A/S.
Abstract: Previously, studies suggest that CD4+ effector T-cell subsets participate in allograft rejection. However, the dynamic changes and relative roles of these CD4+ effector T-cell subsets, especially Th17 cells, have not been systemically examined in patients with acute rejection after cardiac transplantation. In this study, we have studied and compared these CD4+ T-cell subsets in peripheral blood and endomyocardial biopsies (EMB) in patients with stable-graft and acute cellular rejection. We observed that the gene expressions including T-bet, IFN-γ, RORγt, IL-17, IL-23, and FoxP3, the functional marker of Th1, Th17, and FoxP3+ CD4+ T cells, were elevated in EMB samples from patients with acute graft rejection. Accordingly, the percentages of circulating Th1, Th17, and FoxP3+ CD4+ T cells were also significantly increased. The data suggest that Th1, Th17, and FoxP3+ CD4+ T cells are associated with acute graft rejection in patients with cardiac transplantation.
Supporting Information
Table 1. Sequences of oligonucleotide primers used for Q-PCR.
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| CTR_1362_sm_SupplemtaryTable1.doc38.5 KB | Supporting info item |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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