Acute leukaemia immunophenotyping in bone-marrow routine sections
Stefano Aldo Pileri
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorStefano Ascani
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorMarina Milani
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorGiuseppe Visani
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorMilena Piccioli
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorGiulio Fraternali Orcioni
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorSimonetta Poggi
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorElena Sabattini
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorDonatella Santini
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorBrunangelo Falini
Institute of Haematology, Perugia University, Perugia, Italy
Search for more papers by this authorStefano Aldo Pileri
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorStefano Ascani
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorMarina Milani
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorGiuseppe Visani
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorMilena Piccioli
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorGiulio Fraternali Orcioni
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorSimonetta Poggi
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorElena Sabattini
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorDonatella Santini
Service of Pathologic Anatomy and Haematopathology, Institute of Haematology and Clinical Oncology ‘L. & A. Seràgnoli’, Bologna University, Bologna,
Search for more papers by this authorBrunangelo Falini
Institute of Haematology, Perugia University, Perugia, Italy
Search for more papers by this authorAbstract
Immunohistochemistry of acute leukaemias in bone-marrow paraffin sections is commonly thought to be useless because of the poor preservation of many lineage-related markers. The recent development of antibodies against fixative-resistant epitopes and of new antigen retrieval techniques, however, has expanded the possibility of accurately testing routine samples. To assess the relevance of paraffin section phenotyping in lineage determination, 110 examples of acute leukaemia were studied by specific antibodies against CD1a, CD3, CD15, CD20, CD34, CD68, CD79a, TdT, myeloperoxidase, glycophorin A, and factor-VIII-related antigen. The cases included 59 acute myeloid leukaemias, classified according to the FAB cooperative group criteria, 39 precursor B-cell acute lymphoblastic leukaemias (ALLs), seven T-ALLs, and five mixed precursor B-cell/myeloid acute leukaemias. The combination of the markers employed always allowed the identification of the cell lineage (myeloid, lymphoid or mixed) and, in some instances, of phenotypic profiles characteristic of distinct acute leukaemia subtypes. According to the results obtained, bone-marrow biopsy may be regarded as a reliable tool for acute leukaemia diagnosis; this observation is of practical relevance especially for the classification of cases which lack circulating blasts in the peripheral blood or showing dry tap at bone-marrow aspiration.
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