Deregulation of the tumour suppressor genes p14ARF, p15INK4b, p16INK4a and p53 in basal cell carcinoma
P. Kanellou
Laboratory of Virology, Medical School, University of Crete, Heraklion 71100, Crete, Greece
Search for more papers by this authorA. Zaravinos
Laboratory of Virology, Medical School, University of Crete, Heraklion 71100, Crete, Greece
Search for more papers by this authorM. Zioga
A. Sygros Hospital, Department of Dermatology, Medical School, University of Athens, Athens, Greece
Search for more papers by this authorD.A. Spandidos
Laboratory of Virology, Medical School, University of Crete, Heraklion 71100, Crete, Greece
Search for more papers by this authorP. Kanellou
Laboratory of Virology, Medical School, University of Crete, Heraklion 71100, Crete, Greece
Search for more papers by this authorA. Zaravinos
Laboratory of Virology, Medical School, University of Crete, Heraklion 71100, Crete, Greece
Search for more papers by this authorM. Zioga
A. Sygros Hospital, Department of Dermatology, Medical School, University of Athens, Athens, Greece
Search for more papers by this authorD.A. Spandidos
Laboratory of Virology, Medical School, University of Crete, Heraklion 71100, Crete, Greece
Search for more papers by this authorConflicts of interestNone declared.
P.K. and A.Z. contributed equally to this work and should be considered as first authors.
Summary
Background Basal cell carcinoma (BCC) is a locally aggressive slowly growing tumour that rarely metastasizes and is mostly seen in older members of the population.
Objectives To determine the involvement of the tumour suppressor genes p14ARF, p15INK4b, p16INK4a and p53 in BCC.
Methods We investigated the integrity of the CDKN2A locus in 15 BCC samples by analysing the presence of allelic imbalance/loss of heterozygosity (LOH). Moreover, we studied the mRNA expression levels of the tumour suppressor genes p14ARF, p15INK4b, p16INK4a and p53 in the BCC samples and compared them with mRNA levels in the corresponding normal tissue. The presence of mutations was examined by sequencing for exons 1a and 2 of p16INK4a.
Results We found LOH in one BCC sample for the marker D9S1748. A polymorphism (G442A) of exon 2 was detected in three cases. p14ARF, p15INK4b and p53 presented high expression levels, whereas p16INK4a exhibited low mRNA levels compared with the corresponding normal tissue. Significant correlations were detected among the genes studied.
Conclusions Our results demonstrate a different expression profile between p16INK4a and p14ARF, p15INK4b and p53 in BCC. Moreover, we found a low percentage of LOH and of a polymorphic sequence variant (Ala148Thr) for the CDKN2A locus.
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