DNA typing of cyanoacrylate fumed latent fingerprints using GlobalFiler™ and ForenSeq™ Signature Prep kits
Sara R. Bodnar MS
Department of Forensic and Investigative Science, West Virginia University, Morgantown, West Virginia, USA
Search for more papers by this authorCoral Smith MS
Department of Forensic and Investigative Science, West Virginia University, Morgantown, West Virginia, USA
Search for more papers by this authorAlekhlas A. Alsharji BS
Department of Forensic and Investigative Science, West Virginia University, Morgantown, West Virginia, USA
Search for more papers by this authorTina Moroose MS
Department of Forensic and Investigative Science, West Virginia University, Morgantown, West Virginia, USA
Search for more papers by this authorCasper Venter PhD
Department of Forensic and Investigative Science, West Virginia University, Morgantown, West Virginia, USA
Search for more papers by this authorCorresponding Author
Arati Iyengar PhD
Department of Forensic and Investigative Science, West Virginia University, Morgantown, West Virginia, USA
Correspondence
Arati Iyengar, Department of Forensic and Investigative Science, West Virginia University, Morgantown, WV 26505, USA.
Email: [email protected]
Search for more papers by this authorSara R. Bodnar MS
Department of Forensic and Investigative Science, West Virginia University, Morgantown, West Virginia, USA
Search for more papers by this authorCoral Smith MS
Department of Forensic and Investigative Science, West Virginia University, Morgantown, West Virginia, USA
Search for more papers by this authorAlekhlas A. Alsharji BS
Department of Forensic and Investigative Science, West Virginia University, Morgantown, West Virginia, USA
Search for more papers by this authorTina Moroose MS
Department of Forensic and Investigative Science, West Virginia University, Morgantown, West Virginia, USA
Search for more papers by this authorCasper Venter PhD
Department of Forensic and Investigative Science, West Virginia University, Morgantown, West Virginia, USA
Search for more papers by this authorCorresponding Author
Arati Iyengar PhD
Department of Forensic and Investigative Science, West Virginia University, Morgantown, West Virginia, USA
Correspondence
Arati Iyengar, Department of Forensic and Investigative Science, West Virginia University, Morgantown, WV 26505, USA.
Email: [email protected]
Search for more papers by this authorPresentations: Presented in part at the 2022 International Symposium on Human Identification (ISHI), October 31–November 3, in Washington, DC, USA; and at the 34th International Symposium on Human Identification (ISHI), September 18–21, 2003, in Denver, CO, USA.
Abstract
DNA typing of latent fingerprints is highly desirable to increase chances of individualization. We recovered DNA from Cyanoacrylate (CA) fumed fingerprints and used both GlobalFiler™ and ForenSeq™ DNA Signature Prep kits for DNA typing. For GlobalFiler™, samples were processed using a protocol modified for Low Template (LT)-DNA samples (half-volume reactions, 30 cycles) while for ForenSeq™ DNA Signature Prep, samples were processed using a standard protocol and fluorometer-based library quantitation. We evaluated genotyping success and quality of profiles in terms of completeness, Peak Height Ratio/Allele Coverage Ratio, presence of PCR artifacts and drop-in alleles. With GlobalFiler™, average autosomal STR (aSTR) profile completeness was 44.4% with 2–20 pg, 54.3% with 22–60 pg, and 95% with 64–250 pg DNA input. CODIS uploadable profiles were obtained in 2/10, 3/11, and 11/12 samples in these ranges. With ForenSeq™ DNA Signature Prep, average aSTR profile completeness was 19.7% with 1–20 pg and 45.2% with 22–47 pg but increased to 78.3% with 68–122 pg and 86.7% with 618–1000 pg DNA input. Uploadable profiles were obtained in 0/12, 4/11, 4/7, and 3/3 samples for these ranges. Results show very high sensitivity using both kits. Half-volume reactions and 30 cycles had minimal negative effect on Globalfiler™ profile quality, providing support for wider use after validation experiments to routinely improve results from LT samples. A standard protocol for the ForenSeq™ DNA Signature Prep kit was also highly successful with LT DNA obtained from CA-fumed fingerprints with additional information from isometric STR alleles and other markers.
CONFLICT OF INTEREST STATEMENT
The authors have no conflits of interest to report.
Supporting Information
| Filename | Description |
|---|---|
| jfo15566-sup-0001-FigureS1.tiffTIFF image, 7.5 MB |
Figure S1 Average amounts of DNA recovered using 4N6 FLOQSwabsTM and cotton swabs in ng/μL. Horizontal line within box represents median. Filled circles represent means and open circles represent outliers. |
| jfo15566-sup-0002-FigureS2.tiffTIFF image, 9.9 MB |
Figure S2 Read count for each aSTR locus and amelogenin using ForenSeqTM. Horizontal line within box represents median. Filled circles represent means and open circles represent outliers. |
| jfo15566-sup-0003-FigureS3.tiffTIFF image, 9.9 MB |
Figure S3 Read count for each Y-STR locus using ForenSeqTM. Horizontal line within box represents median. Filled circles represent means and open circles represent outliers. |
| jfo15566-sup-0004-FigureS4.tiffTIFF image, 9.9 MB |
Figure S4 Read count for each X-STR locus using ForenSeqTM. Horizontal line within box represents median. Filled circles represent means and open circles represent outliers. |
| jfo15566-sup-0005-FigureS5.tiffTIFF image, 13.9 MB |
Figure S5 Read count for each iiSNP locus using ForenSeqTM. Horizontal line within box represents median. Filled circles represent means and open circles represent outliers. |
| jfo15566-sup-0006-TablesS1-S2.xlsxExcel 2007 spreadsheet , 21.1 KB |
Tables S1–S2 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
REFERENCES
- 1van Oorschot RAH, Jones MK. DNA fingerprints from fingerprints. Nature. 1997; 387: 767. https://doi.org/10.1038/42838
- 2Daly DJ, Murphy C, McDermott SD. The transfer of touch DNA from hands to glass, fabric and wood. Forensic Sci Int Genet. 2012; 6: 41–46. https://doi.org/10.1016/j.fsigen.2010.12.016
- 3Buckingham AK, Harvey ML, van Oorschot RAH. The origin of unknown source DNA from touched objects. Forensic Sci Int Genet. 2016; 25: 26–33. https://doi.org/10.1016/j.fsigen.2016.07.015
- 4Hefetz I, Einot N, Faerman M, Horowitz M, Almog J. Touch DNA: the effect of the deposition pressure on the quality of latent fingermarks and STR profiles. Forensic Sci Int Genet. 2019; 38: 105–112. https://doi.org/10.1016/j.fsigen.2018.10.016
- 5Goray M, Eken E, Mitchell RJ, van Oorschot RAH. Secondary DNA transfer of biological substances under varying test conditions. Forensic Sci Int Genet. 2010; 4: 62–67. https://doi.org/10.1016/j.fsigen.2009.05.001
- 6Fonneløp AE, Egeland T, Gill P. Secondary and subsequent DNA transfer during criminal investigation. Forensic Sci Int Genet. 2015; 17: 155–162. https://doi.org/10.1016/j.fsigen.2015.05.009
- 7Helmus J, Bajanowski T, Poetsch M. DNA transfer—a never ending story. A study on scenarios involving a second person as carrier. Int J Legal Med. 2016; 130: 121–125. https://doi.org/10.1007/s00414-015-1284-1
- 8Kaesler T, Kirkbride KP, Linacre A. Persistence of touch DNA on commonly encountered substrates in different storage conditions. Forensic Sci Int. 2023; 348:111728. https://doi.org/10.1016/j.forsciint.2023.111728
- 9Lim S, Subhani Z, Daniel B, Frascione N. Touch DNA-the prospect of DNA profiles from cables. Sci Justice. 2016; 56: 210–215. https://doi.org/10.1016/j.scijus.2016.02.002
- 10Wargacki SP, Lewis LA, Dadmun MD. Understanding the chemistry of the development of latent fingerprints by superglue fuming. J Forensic Sci. 2007; 52(5): 1057–1062. https://doi.org/10.1111/j.1556-4029.2007.00527.x
- 11Velthuis S, de Puit M. Studies toward the development of a positive control test for the cyanoacrylate fuming technique using artificial sweat. J Forensic Identif. 2011; 61: 16–29.
- 12Raymond JJ, Roux C, Pasquier E, Sutton J, Lennard C. The effect of common fingerprint detection techniques on the DNA typing of fingerprints deposited on different surfaces. J Forensic Identif. 2004; 54: 22–44.
- 13Silvia AL, Tom KR, Breslin K, Donfack J. A comparison of two methods for the recovery of fingerprint DNA on adhesive tapes. Forensic Genom. 2023; 3: 112–122. https://doi.org/10.1089/forensic.2023.0010
- 14Joy J, Cox JO, Hudson BC, Armstrong J, Miller MT, Dawson CT. Comparison of cyanoacrylate fuming techniques of bloody and latent fingerprints and the examination of subsequent DNA success. J Forensic Identif. 2020; 70: 171–185.
- 15Carlin M, Nickel R, Halstead K, Viray J, Hall A, Ehrlich A. Quantifying DNA loss in laboratory-created latent prints due to fingerprint processing. Forensic Sci Int. 2023; 344:111595. https://doi.org/10.1016/j.forsciint.2023.111595
- 16Kanokwongnuwut P, Kirkbride KP, Kobus H, Linacre A. Enhancement of fingermarks and visualizing DNA. Forensic Sci Int. 2019; 300: 99–105. https://doi.org/10.1016/j.forsciint.2019.04.035
- 17Bille TW, Cromartie C, Farr M. Effects of cyanoacrylate fuming, time after recovery, and location of biological material on the recovery and analysis of DNA from post-blast pipe bomb fragments. J Forensic Sci. 2009; 54(5): 1059–1067. https://doi.org/10.1111/j.1556-4029.2009.01128.x
- 18Khuu A, Chadwick S, Moret S, Spindler X, Gunn P, Roux C. Impact of one-step luminescent cyanoacrylate treatment on subsequent DNA analysis. Forensic Sci Int. 2018; 286: 1–7. https://doi.org/10.1016/j.forsciint.2018.02.015
- 19Solomon AD, Hytinen ME, McClain AM, Miller MT, Dawson CT. An optimized DNA analysis workflow for the sampling, extraction, and concentration of DNA obtained from archived latent fingerprints. J Forensic Sci. 2018; 63(1): 47–57. https://doi.org/10.1111/1556-4029.13504
- 20Feine I, Shpitzen M, Geller B, Salmon E, Peleg T, Roth J, et al. Acetone facilitated DNA sampling from electrical tapes improves DNA recovery and enables latent fingerprints development. Forensic Sci Int. 2017; 276: 107–110. https://doi.org/10.1016/j.forsciint.2017.04.023
- 21Thomasma SM, Foran DR. The influence of swabbing solutions on DNA recovery from touch samples. J Forensic Sci. 2013; 58(2): 465–469. https://doi.org/10.1111/1556-4029.12036
- 22Burrill J, Kombara A, Daniel B, Frascione N. Exploration of cell-free DNA (cfDNA) recovery for touch deposits. Forensic Sci Int Genet. 2021; 51:102431. https://doi.org/10.1016/j.fsigen.2020.102431
- 23Risoluti R, Filetti V, Iuliano G, Niola L, Schiavone S, Arcudi G, et al. Updating procedures in forensic chemistry: one step cyanoacrylate method to develop latent fingermarks and subsequent DNA profiling. Microchem J. 2019; 147: 478–486. https://doi.org/10.1016/j.microc.2019.03.056
- 24Ludeman MJ, Zhong C, Mulero JJ, Lagacé RE, Hennessy LK, Short ML, et al. Developmental validation of GlobalFiler™ PCR amplification kit: a 6-dye multiplex assay designed for amplification of casework samples. Int J Legal Med. 2018; 132: 1555–1573. https://doi.org/10.1007/s00414-018-1817-5
- 25Lin S-W, Li C, Ip SCY. A selection guide for the new generation 6-dye DNA profiling systems. Forensic Sci Int Genet. 2017; 30: 34–42. https://doi.org/10.1016/j.fsigen.2017.05.010
- 26Harrel M, Gangitano D, Hughes-Stamm S. The effects of extra PCR cycles when amplifying skeletal samples with the GlobalFiler® PCR amplification kit. Int J Legal Med. 2019; 133: 745–750. https://doi.org/10.1007/s00414-018-1860-2
- 27Jiang X, Guo F, Jia F, Jin P, Sun Z. Development of a 20-locus fluorescent multiplex system as a valuable tool for national DNA database. Forensic Sci Int Genet. 2013; 7: 279–289. https://doi.org/10.1016/j.fsigen.2012.11.006
- 28Liu F, Jia F, Sun F, Zhao B, Shen H. Validation of a multiplex amplification system of 19 autosomal STRs and 27 Y-STRs. Forensic Sci Res. 2020; 5: 292–299. https://doi.org/10.1080/20961790.2019.1665158
10.1080/20961790.2019.1665158 Google Scholar
- 29McNevin D, Edson J, Robertson J, Austin JJ. Reduced reaction volumes and increased Taq DNA polymerase concentration improve STR profiling outcomes from a real-world low template DNA source: Telogen hairs. Forensic Sci Med Pathol. 2015; 11: 326–338. https://doi.org/10.1007/s12024-015-9679-3
- 30Ensenberger MG, Lenz KA, Matthies LK, Hadinoto GM, Schienman JE, Przech AJ, et al. Developmental validation of the PowerPlex® fusion 6C system. Forensic Sci Int Genet. 2016; 21: 134–144. https://doi.org/10.1016/j.fsigen.2015.12.011
- 31Almohammed E, Hadi S. Internal validation of GlobalFiler™ kit using reduced reaction volume. Forensic Sci Int Genet Suppl Ser. 2019; 7: 878–883. https://doi.org/10.1016/j.fsigss.2019.11.009
- 32Iqbal MU, Butt MA, Siddique N, Ahmad QL, Ahmad S, Amjad M, et al. Internal validation of reduced polymerase chain reaction volume for the amplification of nonquantified DNA extracts from reference samples. Forensic Genom. 2021; 1: 87–90. https://doi.org/10.1089/forensic.2021.0007
- 33Brito P, Gouveia N, Bogas V, Bento AM, Balsa F, Lopes V, et al. Evaluation and comparative analysis on reduction of Globalfiler™ reaction volume in low template samples. Forensic Sci Int Genet Suppl Ser. 2017; 6: e362–e364. https://doi.org/10.1016/j.fsigss.2017.09.109
10.1016/j.fsigss.2017.09.109 Google Scholar
- 34Hollard C, Ausset L, Chantrel Y, Jullien S, Clot M, Faivre M, et al. Automation and developmental validation of the ForenSeq™ DNA signature preparation kit for high-throughput analysis in forensic laboratories. Forensic Sci Int Genet. 2019; 40: 37–45. https://doi.org/10.1016/j.fsigen.2019.01.010
- 35England R, Nancollis G, Stacey J, Sarman A, Min J, Harbison SA. Compatibility of the ForenSeq™ DNA signature prep kit with laser microdissected cells: an exploration of issues that arise with samples containing low cell numbers. Forensic Sci Int Genet. 2020; 47:102278. https://doi.org/10.1016/j.fsigen.2020.102278
- 36Guo F, Yu J, Zhang L, Li J. Massively parallel sequencing of forensic STRs and SNPs using the Illumina® ForenSeq™ DNA signature prep kit on the MiSeq FGx™ forensic genomics system. Forensic Sci Int Genet. 2017; 31: 135–148. https://doi.org/10.1016/j.fsigen.2017.09.003
- 37Fattorini P, Previderé C, Carboni I, Marrubini G, Sorçaburu-Cigliero S, Grignani P, et al. Performance of the ForenSeqTM DNA signature prep kit on highly degraded samples. Electrophoresis. 2017; 38: 1163–1174. https://doi.org/10.1002/elps.201600290
- 38Senst A, Caliebe A, Scheurer E, Schulz I. Validation and beyond: next generation sequencing of forensic casework samples including challenging tissue samples from altered human corpses using the MiSeq FGx system. J Forensic Sci. 2022; 67(4): 1382–1398. https://doi.org/10.1111/1556-4029.15028
- 39McElhoe JA, Holland MM, Makova KD, Su MS-W, Paul IM, Baker CH, et al. Development and assessment of an optimized next-generation DNA sequencing approach for the mtgenome using the Illumina MiSeq. Forensic Sci Int Genet. 2014; 13: 20. https://doi.org/10.1016/j.fsigen.2014.05.007
- 40England R, Harbison S. A review of the method and validation of the MiSeq FGx™ forensic genomics solution. WIREs Forensic Sci. 2019; 2:e1351. https://doi.org/10.1002/wfs2.1351
10.1002/wfs2.1351 Google Scholar
- 41Comte J, Baechler S, Gervaix J, Lock E, Milon M-P, Delémont O, et al. Touch DNA collection – performance of four different swabs. Forensic Sci Int Genet. 2019; 43:102113. https://doi.org/10.1016/j.fsigen.2019.06.014
- 42Dadhania A, Nelson M, Caves G, Santiago R, Podini D. Evaluation of Copan 4N6FLOQSwabs™ used for crime scene evidence collection. Forensic Sci Int Genet Suppl Ser. 2013; 4: e336–e337. https://doi.org/10.1016/j.fsigss.2013.10.171
10.1016/j.fsigss.2013.10.171 Google Scholar
- 43Bruijns BB, Tiggelaar RM, Gardeniers H. The extraction and recovery efficiency of pure DNA for different types of swabs. J Forensic Sci. 2018; 63(5): 1492–1499. https://doi.org/10.1111/1556-4029.13837
- 44Seiberle I, Währer J, Kron S, Flury K, Girardin M, Schocker A, et al. Collaborative swab performance comparison and the impact of sampling solution volumes on DNA recovery. Forensic Sci Int Genet. 2022; 59:102716. https://doi.org/10.1016/j.fsigen.2022.102716
- 45Comment D, Gouy A, Zingg C, Zieger M. A holistic approach for the selection of forensic DNA swabs. Forensic Sci Int. 2023; 348:111737. https://doi.org/10.1016/j.forsciint.2023.111737
- 46Schulte J, Rittiner N, Seiberle I, Kron S, Schulz I. Collecting touch DNA from glass surfaces using different sampling solutions and volumes: immediate and storage effects on genetic STR analysis. J Forensic Sci. 2023; 68(4): 1133–1147. https://doi.org/10.1111/1556-4029.15305
- 47Sweet D, Lorente M, Lorente JA, Valenzuela A, Villanueva E. An improved method to recover saliva from human skin: the double swab technique. J Forensic Sci. 1997; 42(2): 320–322. https://doi.org/10.1520/JFS14120J
- 48Poetsch M, Konrad H, Helmus J, Bajanowski T, von Wurmb-Schwark N. Does zero really mean nothing? —first experiences with the new PowerQuant™ system in comparison to established real-time quantification kits. Int J Legal Med. 2016; 130: 935–940. https://doi.org/10.1007/s00414-016-1352-1
- 49Watkins DRL, Myers D, Xavier HE, Marciano MA. Revisiting single cell analysis in forensic science. Sci Rep. 2021; 11:7054. https://doi.org/10.1038/s41598-021-86271-6
- 50Peacock R, Sheeba E, Melia L, Vintiner S. Investigations into improving the standard DNA testing of trace samples at ESR. Aust J Forensic Sci. 2019; 51: S31–S34. https://doi.org/10.1080/00450618.2019.1572787
- 51Perry J, Munshi T, Haizel T, Iyavoo S. Validation of reduced volume VeriFiler™ express PCR amplification kit for buccal swab samples extracted using prep-n-go™ buffer. J Forensic Sci. 2022; 67(5): 1971–1978. https://doi.org/10.1111/1556-4029.15084
- 52Caputo M, Bobillo M, Sala A, Corach D. Optimizing direct amplification of forensic commercial kits for STR determination. J Forensic Leg Med. 2017; 47: 17–23. https://doi.org/10.1016/j.jflm.2017.01.003
- 53Alwi AR, Mahat NA, Salleh FM, Ishar SM, Kamaluddin MR, Rashid MRA. Internal validation of reduced PCR reaction volume of the Qiagen investigator® Argus X-12 QS kit from blood samples on FTA cards. J Forensic Sci. 2023; 68: 2103–2115. https://doi.org/10.1111/1556-4029.15370
- 54Prasad E, Atwood L, van Oorschot RAH, McNevin D, Barash M, Raymond J. Trace DNA recovery rates from firearms and ammunition as revealed by casework data. Aust J Forensic Sci. 2023; 55: 73–88. https://doi.org/10.1080/00450618.2021.1939783
- 55Roeder AD, Elsmore P, Greenhalgh M, McDonald A. Maximizing DNA profiling success from sub-optimal quantities of DNA: a staged approach. Forensic Sci Int Genet. 2009; 3: 128–137. https://doi.org/10.1016/j.fsigen.2008.12.004
- 56Morgan AG, Prinz M. Development of improved DNA collection and extraction methods for handled documents. Genes. 2023; 14:761. https://doi.org/10.3390/genes14030761
- 57Sessa F, Salerno M, Bertozzi G, Messina G, Ricci P, Ledda C, et al. Touch DNA: impact of handling time on touch deposit and evaluation of different recovery techniques: an experimental study. Sci Rep. 2019; 9:9542. https://doi.org/10.1038/s41598-019-46051-9
- 58Jansson L, Swensson M, Gifvars E, Hedell R, Forsberg C, Ansell R, et al. Individual shedder status and the origin of touch DNA. Forensic Sci Int Genet. 2022; 56:102626. https://doi.org/10.1016/j.fsigen.2021.102626
- 59Henry L, Zieger M. Self- and non-self-DNA on hands and sleeve cuffs. Int J Legal Med. 2023; 138: 757–766. https://doi.org/10.1007/s00414-023-03124-9
- 60Fantinato C, Gill P, Fonneløp AE. Non-self DNA on the neck: a 24 hours time-course study. Forensic Sci Int Genet. 2022; 57:102661. https://doi.org/10.1016/j.fsigen.2022.102661
- 61Hwa H-L, Wu M-Y, Chung W-C, Ko T-M, Lin C-P, Yin H-I, et al. Massively parallel sequencing analysis of nondegraded and degraded DNA mixtures using the ForenSeq™ system in combination with EuroForMix software. Int J Legal Med. 2019; 133: 25–37. https://doi.org/10.1007/s00414-018-1961-y
- 62Sharma V, Jani K, Khosla P, Butler E, Siegel D, Wurmbach E. Evaluation of ForenSeq™ signature prep kit B on predicting eye and hair coloration as well as biogeographical ancestry by using universal analysis software (UAS) and available web-tools. Electrophoresis. 2019; 40: 1353–1364. https://doi.org/10.1002/elps.201800344
- 63Sharma V, van der Plaat DA, Liu Y, Wurmbach E. Analyzing degraded DNA and challenging samples using the ForenSeq™ DNA signature prep kit. Sci Justice. 2020; 60: 243–252. https://doi.org/10.1016/j.scijus.2019.11.004
- 64Marcińska M, Wróbel M, Parys-Proszek A, Kupiec T. Evaluation of the performance of the beta version of the ForenSeq DNA signature prep kit on the MiSeq FGx forensic genomics system. Forensic Sci Int Genet Suppl Ser. 2019; 7: 585–586. https://doi.org/10.1016/j.fsigss.2019.10.099
- 65Jäger AC, Alvarez ML, Davis CP, Guzmán E, Han Y, Way L, et al. Developmental validation of the MiSeq FGx forensic genomics system for targeted next generation sequencing in forensic DNA casework and database laboratories. Forensic Sci Int Genet. 2017; 28: 52–70. https://doi.org/10.1016/j.fsigen.2017.01.011
- 66Hussing C, Huber C, Bytyci R, Mogensen HS, Morling N, Børsting C. Sequencing of 231 forensic genetic markers using the MiSeq FGx™ forensic genomics system – an evaluation of the assay and software. Forensic Sci Res. 2018; 3: 111–123. https://doi.org/10.1080/20961790.2018.1446672
- 67Churchill JD, Schmedes SE, King JL, Budowle B. Evaluation of the Illumina® Beta version ForenSeq™ DNA signature prep kit for use in genetic profiling. Forensic Sci Int Genet. 2016; 20: 20–29. https://doi.org/10.1016/j.fsigen.2015.09.009
- 68Müller P, Sell C, Hadrys T, Hedman J, Bredemeyer S, Laurent F-X, et al. Inter-laboratory study on standardized MPS libraries: evaluation of performance, concordance, and sensitivity using mixtures and degraded DNA. Int J Legal Med. 2020; 134: 185–198. https://doi.org/10.1007/s00414-019-02201-2
- 69Silvia AL, Shugarts N, Smith J. A preliminary assessment of the ForenSeq™ FGx system: next generation sequencing of an STR and SNP multiplex. Int J Legal Med. 2017; 131: 73–86. https://doi.org/10.1007/s00414-016-1457-6
- 70Sharma V, Chow HY, Siegel D, Wurmbach E. Qualitative and quantitative assessment of Illumina's forensic STR and SNP kits on MiSeq FGx™. PLoS One. 2017; 12:e0187932. https://doi.org/10.1371/journal.pone.0187932
- 71Almalki N, Chow HY, Sharma V, Hart K, Siegel D, Wurmbach E. Systematic assessment of the performance of Illumina's MiSeq FGx™ forensic genomics system. Electrophoresis. 2017; 38: 846–854. https://doi.org/10.1002/elps.201600511
- 72Churchill JD, Novroski NMM, King JL, Seah LH, Budowle B. Population and performance analyses of four major populations with Illumina's FGx forensic genomics system. Forensic Sci Int Genet. 2017; 30: 81–92. https://doi.org/10.1016/j.fsigen.2017.06.004
