An Optimized DNA Analysis Workflow for the Sampling, Extraction, and Concentration of DNA obtained from Archived Latent Fingerprints†
April D. Solomon M.S.
Department of Forensic Science, Virginia Commonwealth University, PO Box 843079, 1015 Floyd Avenue (Room 2011), Richmond, VA, 23284
Search for more papers by this authorMadison E. Hytinen B.S.
Department of Forensic Science, Virginia Commonwealth University, PO Box 843079, 1015 Floyd Avenue (Room 2011), Richmond, VA, 23284
Search for more papers by this authorAryn M. McClain M.S.
Department of Forensic Science, Virginia Commonwealth University, PO Box 843079, 1015 Floyd Avenue (Room 2011), Richmond, VA, 23284
Search for more papers by this authorMarilyn T. Miller Ed.D.
Department of Forensic Science, Virginia Commonwealth University, PO Box 843079, 1015 Floyd Avenue (Room 2011), Richmond, VA, 23284
Search for more papers by this authorCorresponding Author
Tracey Dawson Cruz Ph.D.
Department of Forensic Science, Virginia Commonwealth University, PO Box 843079, 1015 Floyd Avenue (Room 2011), Richmond, VA, 23284
Additional information and reprint requests:
Tracey Dawson Cruz, Ph.D.
Department of Forensic Science
Virginia Commonwealth University
PO Box 843079
10 Floyd Avenue (Room 2011)
Richmond, VA 23284
E-mail: [email protected]
Search for more papers by this authorApril D. Solomon M.S.
Department of Forensic Science, Virginia Commonwealth University, PO Box 843079, 1015 Floyd Avenue (Room 2011), Richmond, VA, 23284
Search for more papers by this authorMadison E. Hytinen B.S.
Department of Forensic Science, Virginia Commonwealth University, PO Box 843079, 1015 Floyd Avenue (Room 2011), Richmond, VA, 23284
Search for more papers by this authorAryn M. McClain M.S.
Department of Forensic Science, Virginia Commonwealth University, PO Box 843079, 1015 Floyd Avenue (Room 2011), Richmond, VA, 23284
Search for more papers by this authorMarilyn T. Miller Ed.D.
Department of Forensic Science, Virginia Commonwealth University, PO Box 843079, 1015 Floyd Avenue (Room 2011), Richmond, VA, 23284
Search for more papers by this authorCorresponding Author
Tracey Dawson Cruz Ph.D.
Department of Forensic Science, Virginia Commonwealth University, PO Box 843079, 1015 Floyd Avenue (Room 2011), Richmond, VA, 23284
Additional information and reprint requests:
Tracey Dawson Cruz, Ph.D.
Department of Forensic Science
Virginia Commonwealth University
PO Box 843079
10 Floyd Avenue (Room 2011)
Richmond, VA 23284
E-mail: [email protected]
Search for more papers by this authorAbstract
DNA profiles have been obtained from fingerprints, but there is limited knowledge regarding DNA analysis from archived latent fingerprints—touch DNA “sandwiched” between adhesive and paper. Thus, this study sought to comparatively analyze a variety of collection and analytical methods in an effort to seek an optimized workflow for this specific sample type. Untreated and treated archived latent fingerprints were utilized to compare different biological sampling techniques, swab diluents, DNA extraction systems, DNA concentration practices, and post-amplification purification methods. Archived latent fingerprints disassembled and sampled via direct cutting, followed by DNA extracted using the QIAamp® DNA Investigator Kit, and concentration with Centri-Sep™ columns increased the odds of obtaining an STR profile. Using the recommended DNA workflow, 9 of the 10 samples provided STR profiles, which included 7–100% of the expected STR alleles and two full profiles. Thus, with carefully selected procedures, archived latent fingerprints can be a viable DNA source for criminal investigations including cold/postconviction cases.
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