Psoriatic T cells reduce epidermal turnover time and affect cell proliferation contributed from differential gene expression
Junqin Li
Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China
Search for more papers by this authorXinhua Li
Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China
Search for more papers by this authorRuixia Hou
Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China
Search for more papers by this authorRuifeng Liu
Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China
Search for more papers by this authorXincheng Zhao
Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China
Search for more papers by this authorFeng Dong
Department of Dermatology, Changzhi City Second People's Hospital, Changzhi, China
Search for more papers by this authorChunfang Wang
Laboratory Animal Center, Shanxi Medical University, Taiyuan, China
Search for more papers by this authorGuohua Yin
Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China
Search for more papers by this authorCorresponding Author
Kaiming Zhang
Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China
Correspondence: Kaiming Zhang, M.D., Institute of Dermatology, Taiyuan City Center Hospital, 1 Dong San Dao Xiang, Jiefang Road, Taiyuan, Shanxi 030009, China. Email: [email protected]Search for more papers by this authorJunqin Li
Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China
Search for more papers by this authorXinhua Li
Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China
Search for more papers by this authorRuixia Hou
Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China
Search for more papers by this authorRuifeng Liu
Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China
Search for more papers by this authorXincheng Zhao
Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China
Search for more papers by this authorFeng Dong
Department of Dermatology, Changzhi City Second People's Hospital, Changzhi, China
Search for more papers by this authorChunfang Wang
Laboratory Animal Center, Shanxi Medical University, Taiyuan, China
Search for more papers by this authorGuohua Yin
Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China
Search for more papers by this authorCorresponding Author
Kaiming Zhang
Institute of Dermatology, Taiyuan City Center Hospital, Taiyuan, China
Correspondence: Kaiming Zhang, M.D., Institute of Dermatology, Taiyuan City Center Hospital, 1 Dong San Dao Xiang, Jiefang Road, Taiyuan, Shanxi 030009, China. Email: [email protected]Search for more papers by this authorAbstract
Psoriasis is mediated primarily by T cells, which reduce epidermal turnover time and affect keratinocyte proliferation. We aimed to identify differentially expressed genes (DEG) in T cells from normal, five pairs of monozygotic twins concordant or discordant for psoriasis, to determine whether these DEG may account for the influence to epidermal turnover time and keratinocyte proliferation. The impact of T cells on keratinocyte proliferation and epidermal turnover time were investigated separately by immunohistochemistry and cultured with 3H-TdR. mRNA expression patterns were investigated by RNA sequencing and verified by real-time reverse transcription polymerase chain reaction. After co-culture with psoriatic T cells, the expression of Ki-67, c-Myc and p53 increased, while expression of Bcl-2 and epidermal turnover time decreased. There were 14 DEG which were found to participate in the regulation of cell proliferation or differentiation. Psoriatic T cells exhibited the ability to decrease epidermal turnover time and affect keratinocyte proliferation because of the differential expression of PPIL1, HSPH1, SENP3, NUP54, FABP5, PLEKHG3, SLC9A9 and CHCHD4.
Supporting Information
| Filename | Description |
|---|---|
| jde12961-sup-0001-TableS1-S3.docWord document, 156.5 KB | Table S1. Specimen sources Table S2. Primers for real time reverse transcription polymerase chain reaction (RT–PCR) Table S3. Differentially expressed genes |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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