Volume 69, Issue 5 pp. 866-871

Structures of Enterovirus 71 3C proteinase (strain E2004104-TW-CDC) and its complex with rupintrivir

First published: 01 May 2013
Citations: 1
Jian Li, e-mail: [email protected]; Tianwei Lin, e-mail: [email protected]

Abstract

The crystal structure of 3C proteinase (3Cpro) from Enterovirus 71 (EV71) was determined in space group C2221 to 2.2 Å resolution. The fold was similar to that of 3Cpro from other picornaviruses, but the difference in the β-ribbon reported in a previous structure was not observed. This β-ribbon was folded over the substrate-binding cleft and constituted part of the essential binding sites for interaction with the substrate. The structure of its complex with rupintrivir (AG7088), a peptidomimetic inhibitor, was also characterized in space group P212121 to 1.96 Å resolution. The inhibitor was accommodated without any spatial hindrance despite the more constricted binding site; this was confirmed by functional assays, in which the inhibitor showed comparable potency towards EV71 3Cpro and human rhinovirus 3Cpro, which is the target that rupintrivir was designed against.

The full text of this article hosted at iucr.org is unavailable due to technical difficulties.