Crystallization and preliminary X-ray crystallographic studies on recombinant human carnitine acetyltransferase

In this paper, the purification, crystallization and preliminary X-ray crystallographic studies of human carnitine acetyltransferase are reported. Recombinant human carnitine acetyltransferase crystals were grown by the hanging-drop vapor-diffusion method and belong to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 137.65, b = 84.76, c = 57.65 Å and one molecule per asymmetric unit. The intensity data were collected from a cryocooled crystal to 1.6 Å resolution using a conventional X-ray source.