Address correspondence and reprint requests to Prof. Mario Plebani, Department of Laboratory Medicine, University-Hospital of Padova, Via Giustiniani 2, 35128 Padova, Italy (e-mail: [email protected]).Search for more papers by this author

Daniela Basso,

Daniela Basso

Departments of Laboratory Medicine, University of Padova, Padova, Italy

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Graziella Guariso,

Graziella Guariso

Departments of Pediatrics, University of Padova, Padova, Italy

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Michela Fasolo,

Michela Fasolo

Departments of Laboratory Medicine, University of Padova, Padova, Italy

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Marina Pittoni,

Marina Pittoni

Departments of Laboratory Medicine, University of Padova, Padova, Italy

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Stefania Schiavon,

Stefania Schiavon

Departments of Laboratory Medicine, University of Padova, Padova, Italy

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Paola Fogar,

Paola Fogar

Departments of Medical and Surgical Sciences, University of Padova, Padova, Italy

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Eliana Greco,

Eliana Greco

Departments of Laboratory Medicine, University of Padova, Padova, Italy

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Filippo Navaglia,

Filippo Navaglia

Departments of Laboratory Medicine, University of Padova, Padova, Italy

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Carlo-Federico Zambon,

Carlo-Federico Zambon

Departments of Medical and Surgical Sciences, University of Padova, Padova, Italy

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Mario Plebani,

Corresponding Author

Mario Plebani

[email protected]

Departments of Laboratory Medicine, University of Padova, Padova, Italy

First published: 01 November 2006

https://doi.org/10.1097/01.mpg.0000239739.09983.38

Citations: 16

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ABSTRACT

Objectives:

Anti–tissue transglutaminase antibody (anti-tTG) determination using second-generation (human antigen) enzyme-linked immunoassays (ELISAs) is a very accurate test to diagnose celiac disease (CD). In this study, we compared 2 second-generation ELISAs (Celikey tTG; Pharmacia Diagnostics GmbH & Co, Freiburg, Germany, and QuantaLite; Inova Diagnostics, San Diego, CA) and antiendomysial antibodies (EMAs) with a new indirect chemiluminescence immunoassay (LIAISON tTG; DiaSorin S.p.A., Saluggia, Italy) in diagnosing and monitoring CD in children.

Patients and Methods:

Antiendomysial antibodies, anti-tTGs and total immunoglobulin A were measured in the sera of 103 control children, 101 children with histologically proven CD and 31 CD children on gluten-free diet (GFD).

Results:

Anti–tissue transglutaminase antibody mean levels were significantly higher in CD with respect to control or GFD children. The sensitivity value of EMAs, LIAISON tTG, Celikey tTG and QuantaLite in diagnosing CD was 97.7%, 97.0%, 94.1% and 98.0%, respectively, and the corresponding specificity values were 91.1%, 98.1%, 97.1% and 96.1%, respectively. The degree of mucosal destruction (Marsh criteria) was correlated with EMA semiquantification (P < 0.01) and with the circulating levels of anti-tTGs measured using LIAISON (P < 0.05) or QuantaLite (P < 0.01). Twenty-six CD children were followed up from 5 to 25 months after GFD. The circulating levels of anti-tTGs measured with any of the 3 assays significantly dropped after GFD.

Conclusions:

Anti–tissue transglutaminase antibody determination with second-generation ELISAs is as effective as EMAs for CD diagnosis. The novel chemiluminescent method described in the present paper for the detection of anti-tTGs in the diagnosis of CD had the highest sensitivity and specificity values. The anti-tTG test correlates with the degree of mucosal destruction and is suitable for verifying patient compliance to dietary treatment.

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Volume43, Issue5

November 2006

Pages 613-618

A New Indirect Chemiluminescent Immunoassay to Measure Anti–tissue Transglutaminase Antibodies

ABSTRACT

Objectives:

Patients and Methods:

Results:

Conclusions:

REFERENCES

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References

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A New Indirect Chemiluminescent Immunoassay to Measure Anti–tissue Transglutaminase Antibodies

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REFERENCES

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