The number of platelet glycoprotein Ia molecules is associated with the genetically linked 807 C/T and HPA-5 polymorphisms
Javier Corral PhD
Senior Scientist
Onco-Hematology Unit, Hospital General Universitario, Centro Regional de Hemodonación, Murcia, Spain
Search for more papers by this authorJosé Rivera PhD
Senior Scientist and Associate Professor
Onco-Hematology Unit, Hospital General Universitario, Centro Regional de Hemodonación, Murcia, Spain
Search for more papers by this authorRocio González-Conejero PhD
Senior Scientist
Onco-Hematology Unit, Hospital General Universitario, Centro Regional de Hemodonación, Murcia, Spain
Search for more papers by this authorCorresponding Author
Vicente Vicente MD, PhD
Director
Centro Regional de Hemodonación, Murcia, Spain.
Professor of Medicine, Onco-Hematology Unit, Hospital General Universitario, Centro Regional de Hemodonación, Ronda de Garay s/n, 300003 Murcia, Spain.Search for more papers by this authorJavier Corral PhD
Senior Scientist
Onco-Hematology Unit, Hospital General Universitario, Centro Regional de Hemodonación, Murcia, Spain
Search for more papers by this authorJosé Rivera PhD
Senior Scientist and Associate Professor
Onco-Hematology Unit, Hospital General Universitario, Centro Regional de Hemodonación, Murcia, Spain
Search for more papers by this authorRocio González-Conejero PhD
Senior Scientist
Onco-Hematology Unit, Hospital General Universitario, Centro Regional de Hemodonación, Murcia, Spain
Search for more papers by this authorCorresponding Author
Vicente Vicente MD, PhD
Director
Centro Regional de Hemodonación, Murcia, Spain.
Professor of Medicine, Onco-Hematology Unit, Hospital General Universitario, Centro Regional de Hemodonación, Ronda de Garay s/n, 300003 Murcia, Spain.Search for more papers by this authorSupported in part by Fondo de Investigaciones Sanitarias 97/1150 (RG-C) and Asociación de Investigación de Enfermedad Tromboembólica de la Región de Murcia.
Abstract
BACKGROUND: The neutral 807 C/T (Phe224) polymorphism (807 C/T polymorphism) of the glycoprotein (GP)Ia gene has been recently associated with the number of GPIa molecules on the platelet surface. The association of the number of GPIa molecules with other GPIa polymorphisms, such as HPA-5 (Glu/Lys505) (HPA-5 polymorphism), involved in alloimmune thrombocytopenias is unknown.
STUDY DESIGN AND METHODS: The association of the HPA-5 polymorphism with the number of GPIa molecules on the platelet surface in 159 white blood donors was investigated. The genetic linkage between the HPA-5 and the 807 C/T polymorphisms in 316 individuals was also determined.
RESULSTS: Both the 807 C/T and HPA-5 polymorphisms correlate with the number of GPIa molecules on the platelet surface. The 807 T and HPA-5b alleles are associated with increased numbers of GPIa molecules on the platelet surface. Moreover, the HPA-5b allele is genetically linked to 15.8 percent of the 807 C alleles. Therefore, the number of GPIa molecules on the platelet surface is dependent on both GPIa polymorphisms as follows: 807 T/T, HPA-5 a/a > 807 C/T, HPA-5 a/b > 807 C/T, HPA-5 a/a > 807 C/C, HPA-5 a/b > 807 C/C, HPA-5 a/a.
CONCLUSION: Two GPIa polymorphisms (807 C/T and HPA-5) responsible for the variability in the numbers of GPIa/lla molecules on the platelet surface in whites have been identified. Despite the genetic linkage between the two polymorphisms, their influence on the number of GPIa molecules on the platelet surface may occur through different mechanisms.
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