An improved micro-method for obtaining chromosome preparations from individual haemopoietic colonies
Professor Eric G. Wright, MRC Radiation and Genome Stability Unit, Harwell, Oxon OX11 0RD. e-mail: [email protected]
Abstract
A robust method for obtaining chromosome preparations from individual haemopoietic colonies in semi-solid media is described. The accumulation of metaphases and the hypotonic treatment of cells were carried out in the culture dish and individual colonies were transferred onto poly-l-lysine-treated slides and fixed stepwise prior to staining. The technique produced high yields of well-spread metaphases facilitating clonal cytogenetic analysis.
