Long-lasting effects of serotonin deficiency on differentiating peptidergic neurons in the rat suprachiasmatic nucleus
V. Mirochnik
Institute of Normal Physiology, Russian Academy of Medical Sciences, 8 Baltiiskaya str., Moscow, 125315 Russia
Institut Fédératif Jean-Roche, Faculté de Médecine (secteur nord), Université de la Méditerranée, Blvd Pierre Dramard, 13916 Marseille, Cédex 20, France
Search for more papers by this authorO. Bosler
Institut Fédératif Jean-Roche, Faculté de Médecine (secteur nord), Université de la Méditerranée, Blvd Pierre Dramard, 13916 Marseille, Cédex 20, France
Search for more papers by this authorY. Tillet
Unité de Neuroendocrinologie Sexuelle, INRA Nouzilly, 37380 Monnaie, France
Search for more papers by this authorA. Calas
Neurobiologie des Signaux Intercellulaires UMR 7101, Université P. et M. Curie, 7 quai St. Bernard, F 75252 Paris, Cédex 05, France
Search for more papers by this authorCorresponding Author
M. Ugrumov
Institute of Normal Physiology, Russian Academy of Medical Sciences, 8 Baltiiskaya str., Moscow, 125315 Russia
Neurobiologie des Signaux Intercellulaires UMR 7101, Université P. et M. Curie, 7 quai St. Bernard, F 75252 Paris, Cédex 05, France
Laboratory of Hormonal Regulations, Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov str., Moscow, 119991 Russia
*Corresponding author. Tel.: +7 095 135 8842; fax: +7 095 135 8842.
E-mail address:[email protected], [email protected] (M. Ugrumov)
Search for more papers by this authorV. Mirochnik
Institute of Normal Physiology, Russian Academy of Medical Sciences, 8 Baltiiskaya str., Moscow, 125315 Russia
Institut Fédératif Jean-Roche, Faculté de Médecine (secteur nord), Université de la Méditerranée, Blvd Pierre Dramard, 13916 Marseille, Cédex 20, France
Search for more papers by this authorO. Bosler
Institut Fédératif Jean-Roche, Faculté de Médecine (secteur nord), Université de la Méditerranée, Blvd Pierre Dramard, 13916 Marseille, Cédex 20, France
Search for more papers by this authorY. Tillet
Unité de Neuroendocrinologie Sexuelle, INRA Nouzilly, 37380 Monnaie, France
Search for more papers by this authorA. Calas
Neurobiologie des Signaux Intercellulaires UMR 7101, Université P. et M. Curie, 7 quai St. Bernard, F 75252 Paris, Cédex 05, France
Search for more papers by this authorCorresponding Author
M. Ugrumov
Institute of Normal Physiology, Russian Academy of Medical Sciences, 8 Baltiiskaya str., Moscow, 125315 Russia
Neurobiologie des Signaux Intercellulaires UMR 7101, Université P. et M. Curie, 7 quai St. Bernard, F 75252 Paris, Cédex 05, France
Laboratory of Hormonal Regulations, Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov str., Moscow, 119991 Russia
*Corresponding author. Tel.: +7 095 135 8842; fax: +7 095 135 8842.
E-mail address:[email protected], [email protected] (M. Ugrumov)
Search for more papers by this authorAbstract
Serotonin (5-HT, 5-hydroxytryptamine) is known to be an inductor of the brain development [Whitaker-Azmitia, P.M., Druse, M., Walker, P., Lauder, J.M., 1996. Serotonin as a developmental signal. Behav. Brain Res. 73, 19–29; Ugrumov, M.V., 1997. Hypothalamic monoaminergic systems in ontogenesis: development and functional significance. Int. J. Dev. Biol. 41, 809–816]. This study was aimed to test whether it provides long-lasting effects on the differentiating vasoactive intestinal polypeptide (VIP) and vasopressin (VP) neurons of the suprachiasmatic nucleus (SCN) in rats. To this aim, 5-HT was depleted in fetal brain by daily injections of p-chlorophenylalanine (pCPA), an inhibitor of 5-HT synthesis, to pregnant rats from the 13th to the 21st day of gestation. Pregnant rats injected with saline served as controls. The offsprings (males) of pCPA-treated and control pregnant rats were maintained after birth for two months under normal laboratory conditions. Then, the SCN was processed for immunocytochemistry of VIP and VP and in situ hybridization of appropriate mRNAs. There were no differences in concentrations of VIP and VP mRNAs in the SCN in adult offsprings of the 5-HT-depleted pregnant rats compared to the controls. Moreover, 5-HT deficiency did not induce any change in size of VIP-immunoreactive (IR) and VP-IR neurons. Conversely, both the numbers of VIP- and VP-immunoreactive neurons and concentrations of the peptides in cell bodies increased significantly. It is concluded that 5-HT provides long-lasting effects on differentiating VIP and VP neurons in the SCN resulting in attenuated release rather than elevated synthesis of both peptides in adulthood.
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